Antibodies to insulin-like growth factor binding protein and uses thereof

ABSTRACT

Using the proteins of the present invention, DNAs encoding the proteins, and antibodies recognizing the proteins, detection methods for diseases relating to the novel insulin-like growth factor binding proteins of the present invention, as well as diagnostic agents, preventive agents, and therapeutic agents for diseases relating to the proteins of the present invention can be provided.

CROSS-REFERENCES TO RELATED APPLICATIONS

This application is a divisional of application Ser. No. 11/396,430,filed Mar. 30, 2006, which is a divisional of application Ser. No.10/311,129, filed Apr. 9, 2003, which is the National Stage ofInternational Application No. PCT/JP01/05112, filed Jun. 15, 2001 andclaims priority to Japanese Patent Application Number 2000-180214, filedJun. 15, 2000. Each of the aforementioned applications are herebyincorporated by reference in their entirety and for all purposes.

BACKGROUND OF THE INVENTION

1. Technical Field

The present invention relates to novel insulin-like growth factorbinding proteins, DNAs encoding the proteins, and antibodies recognizingthe proteins, as well as detection methods, diagnostic agents, andpreventive agents or therapeutic agents for diseases associated with theproteins.

2. Background Art

Insulin-like growth factor binding proteins (hereinafter, referred to as“IGFBP”) are a group of molecules that were discovered due to the factthat the insulin-like growth factor (hereinafter, referred to as “IGF”)exists in the body fluid as a macromolecular complex. 10 types of thesemolecules, IGFBP-1 to 10, have been reported to exist until now, and areknown to constitute a superfamily (Endocr. Rev., 18, 801 (1997); Prog.Growth Factor Res., 3, 243 (1991); Mol. Reprod. Dev., 35, 368 (1993);Proc. Natl. Acad. Sci. USA, 94, 12981 (1997)).

The existence of direct action by binding to integrin and the like hasbeen also suggested as the function of IGFBP. However, the main actionis predicted to be exhibited by the binding to IGF and insulin toregulate their activity, distribution, metabolism, and the like (Endocr.Rev., 18, 801 (1997); Bio Science Terminology Library Cytokines andGrowth Factors, revised edition p 14-17 (1988)). Specifically, the IGFBPis expected to exhibit its function by suppressing the transport intoblood, extravasation and degradation of IGF, by regulating receptorbinding, and the like.

6 types of molecules, IGFBP-1 to 6, among the IGFBP superfamily havestructural similarity and bind with higher affinity to IGF than toinsulin. Therefore, they are classified into a subfamily ashigh-IGF-affinity IGFBPs (Mol. Endocrinol., 2, 404 (1988); EMBO J., 8,2497 (1989); Mol. Endocrinol., 2, 1176 (1989); Mol. Endocrinol., 4, 1806(1990); Biochem. Biophys. Res. Commun., 176, 219 (1991); J. Biol. Chem.,266, 9043 (1991); J. Biol. Chem., 266, 10646 (1991)).

In humans, the amino acid sequence homology among the moleculesbelonging to the high-IGF-affinity IGFBP subfamily is 49 to 60%.Furthermore, 18 cysteine residues are conserved in five of the IGFBPs,excluding IGFBP-6, and three of these residues to the N-terminal sideform a homologous sequence represented by Gly-Cys-Gly-Cys-Cys-X-X-Cys(SEQ ID NO:26) (X represents an arbitrary amino acid; this homologoussequence is called insulin-like growth factor binding motif(hereinafter, referred to as “IGFBP motif”)) and has been revealed to beinvolved in IGF binding (Prog. Growth Factor Res., 3, 243 (1991)).

IGFBP-1 and 3 are known to both suppress and enhance the action of IGF.Whereas IGFBP-2, 4, and 6 are inhibitory IGF binding proteins andIGFBP-5 as a promoting IGF binding protein. There are two types of IGFs,namely, (1) IGF-I: produced in a growth hormone dependent manner inliver, bone tissue, and the like, and functions as a growth factor thatpromotes physical growth; and (2) IGF-II: expressed in large amounts incentral nervous system, bone tissue, and the like, and is presumed toplay an important role in growth, mainly during the embryonal period.IGFBP-1, 3, and 4 indicate a binding activity similar toward IGF-1 andIGF-II, while IGFBP-2, 5, and 6 indicate a strong binding activitymainly toward IGF-II.

The tissue distribution of high-IGF-affinity IGFBP subfamily moleculesdiffer depending on the molecules. IGFBP-1 is known to mainly exist inamniotic fluid and fetal serum; IGFBP-2 mainly in fetal liver and adultbrain; IGFBP-3 mainly in liver and serum; IGFBP-4 mainly in renalglomeruli, skin, and intestinal epithelium; IGFBP-5 mainly in intestinalepithelium and bone; and IGFBP-6 mainly in skin and heart.

The following findings have been reported regarding the relationshipbetween IGFBPs and pathology of diseases.

The fluctuations of IGF and IGFBP expressions are directly associatedwith the pathophysiology of dwarfism and acromegaly patients. On theother hand, although failure to thrive are often seen among patients ofinfantile chronic renal failure, their growth hormone and IGF expressionlevels are normal, and most of the cause is the functional disorder ofIGF due to the increase of IGFBP-2 and/or IGFBP-3 (Miner. ElectrolyteMrtab., 18, 320 (1992)). IGFBP-4 and 5 have important functions in bonemetabolism. It has been reported that the expression level of IGFBP-5decreases in osteoporosis, and the expression of IGFBP-4 elevates inwomen fracture patients of old age accompanied with the elevation ofparathyroid hormone. Furthermore, although enhancement of paracrineeffect of IGF-1 is observed in compensatory hypertrophy afternephrectomy and small intestine resection, the mRNA level of IGF-1 isinvariant, and free IGF-I increases due to diminished IGFBP-3 expression(J. Fuller, Baillieres Clin. Endocrinol. Metab., 8, 165 (1994)).Moreover, the expression of IGFBP-1 is decreased in malignant tumors ofendometrium, compared to benign tumors (Growth Regul., 3, 74, (1993)).

On the other hand, four molecules in the IGFBP superfamily, IGFBP-7 to10, are structurally similar and are considered to share a commoncharacteristic of having a low affinity to IGFs. Therefore, they areclassified into a separate subfamily as low-IGF-affinity IGFBPs (Proc.Natl. Acad. Sci. USA, 94, 12981 (1997); Cancer Res., 59, 2787 (1999)).

Regarding IGFBP-7, various physiological activities have been reported.Particularly, many reports have been made on the relationship withcancer and its pathology.

While the expression of IGFBP-7 is elevated in aged human epithelialcells (J. Clin. Endocrinol. Metab., 4, 715 (1993)), its expression isdiminished in carcinoma cell lines. Therefore, IGFBP-7 is thought tofunction as a gene involved in cancer suppression activity (Proc. Natl.Acad. Sci. USA, 92, 4472 (1995)). The gene locus of IGFBP-7 on the humanchromosome is 4q12, and its expression is known to enhance by thetreatment of human epithelial cells with retinoic acid (Proc. Natl.Acad. Sci. USA, 92, 4472 (1995)).

In breast cancer tissues, LOH (loss of heterozygosity) at chromosome4q12 to 13 was observed at a frequency of approximately 50%, and theexpression of IGFBP-7 has been confirmed to be decreased (Oncogene, 16,2459 (1998)). The IGFBP-7 expression is also decreased at the mRNA levelin prostate cancer tissues, and no expression is detected particularlyin cell lines derived from malignant prostate cancer (J. Clin.Endocrinol. Metab., 83, 4355 (1998)).

Moreover, when IGFBP-7 is forcedly expressed in prostate cancer cellline in which IGFBP-7 expression is decreased, extension of celldivision time, decrease of colony forming ability in soft agar medium,decrease of tumor forming ability in nude mouse transplantation, andelevation of apoptosis induction rate by drug treatment have beenobserved, which suggests a relationship between the IGFBP-7 expressionand degree of malignancy of prostate cancer (Cancer Res., 59, 2370(1999)).

Concentrations of IGFBP-7 and IGFBP-3 in the cerebrospinal fluid havebeen reported to rise in leukemia patients, and their relationship tothe pathology of leukemia is receiving attention (J. Clin. Endocrinol.Metab., 84, 1283 (1999)).

Due to the enhanced expression of IGFBP-7 in large intestinal cancertissues and cell lines, the relationship of IGFBP-7 with the pathologyof colon cancer is receiving attention (J. Gastroenterology, 33, 213(1998)).

IGFBP-7 expression is decreased in large uterine leiomyoma sites, andthe expression of IGFBP-7 is reported to be elevated in patients whohave received gonadotropin-releasing hormone therapy (J. Reprod.Immunol., 43, 53 (2000)).

The 5′ upstream region of IGFBP-7 gene is methylated and its expressionlevel is decreased in mouse liver cancer cells that have been inducedwith SV40T antigen. Therefore, a mechanism involving methylation of thegene has been proposed for the regulation of the IGFBP-7 expressionassociated with canceration (Biochem. Biophys. Res. Commun., 267, 109(2000)).

The relation between IGFBP-7 and diabetes has also been reported.

A factor (PGI2-stimulating factor, hereinafter, referred to as “PSF”)promoting the production of prostacyclin PGI2 by acting on vascularendothelial cells was shown to be identical to IGFBP-7 (Biochem. J.,303, 591, (1994)). The expression of this factor, which is expressed invascular endothelial cells and smooth muscle cells (Thromb Haemost., 74,1407 (1995)), is reported to be decreased in kidneys and angiopathysites of a type I diabetes model established by streptozotocinadministration (Diabetes, 45, S111 (1996); J. Diabetes & itsComplications, 12, 252 (1998)). Furthermore, decreased expression ofIGFBP-7 at the protein level has also been observed in coronary arterysmooth muscle cells of type II diabetes patient (Diabetes, 46, 1627(1997)). Moreover, the expression level of IGFBP-7 has been confirmed todecrease at the mRNA and protein levels by culturing smooth muscle cellsderived from bovine arteries in high-glucose medium (Diabetes, 46, 1627(1997); Diabetologia, 41, 134 (1998)).

The expression of IGFBP-7 in osteoblasts rises by TGF-β (transforminggrowth factor-β), parathyroid hormone (PTH), and prostaglandin E2(PGE2). Therefore, IGFBP-7 appears to have physiological effect onosteoblasts (Endocrinology, 140, 1998 (1999)). Furthermore, treatment ofosteoblasts with glucocorticoids is reported to suppress the expressionof IGF-I, while enhancing the expression of IGFBP-7 (Endocrinology, 140,228 (1999)).

Furthermore, IGFBP-7 is indicated to have an effect on thedifferentiation into skeletal muscles by suppressing thedifferentiation-promoting action of IGF (Exp. Cell Res., 237, 192(1997); Endocrinology, 141, 100 (2000)).

Since IGFBP-7 was the same molecule as the factor PSF that promotesprostacyclin PGI2 production, a part of the physiological function ofIGFBP-7 is considered to perform as the effector molecule for PGI2.

PGI2, a type of prostaglandin, has strong platelet aggregationinhibitory effect and vasorelaxing effect, and is known to functionantagonistically with TXA2 that has the opposite effect to maintain thehomeostasis within a living body (Br. J. Pharmac., 76, 3 (1982)).Imbalance in TXA2 and PGI2 production, especially decrease in PGI2production causes development of angiopathy in thrombosis andarteriosclerosis (Br. J. Pharmac., 76, 3 (1982)). Regarding thedevelopment and progression of diabetic angiopathy, in addition to theenhancement of platelet-derived TXA2 production (Thromb. Res., 19, 211(1980); J. Lab. Clin. Med., 97, 87 (1981)), decrease of bloodvessel-derived PGI2 production has been confirmed to enhance plateletaggregation in diabetes patients and in laboratory animals with diabetes(Lancet, 1, 325 (1979); Lancet, 2, 1365 (1979); N. Engl. J. Med., 300,366 (1979); Life Sci., 23, 351 (1978)).

Furthermore, PSF is a factor that exists in the blood stream and isreported to stimulate the production of PGI2 in vascular wall (Nature,271, 549 (1978)). Moreover, the level of this factor in blood isreported to be decreased in hemolytic uremic syndrome (Lancet, 2, 871(1978)), thrombotic thrombocytopenic purpa (Lancet, 2, 748 (1979)),sickle cell anemia (Br. J. Haematol., 48, 545 (1981)), acute myocardialinfarction (Coronary, 2, 49 (1985)), diabetic angiopathy (Metabolism,38, 837 (1989); Haemostasis, 16, 447 (1986); Diab. Res. Clin. Pract., 3,243 (1987)), and arteriosclerotic diseases.

More specifically, IGFBP-7 has been demonstrated to exert plateletaggregation inhibitory effect, smooth muscle relaxation effect, andgastric acid secretion inhibitory effect by elevating the PGI2concentration in blood via the promotion of PGI2 production of vascularendothelial cells.

As mentioned above, factors belonging to the IGFBP superfamily have beenrevealed to be involved in various biological phenomena includingregulation of IGF and insulin function, pregnancy, compensatory effectin exhaustive diseases, bone metabolism, differentiation of skeletalmuscle cells, promotion of PGI2 production in vascular endothelialcells, PGI2-mediated inhibition of platelet aggregation, vascular smoothmuscle relaxation, bronchial smooth muscle relaxation, and inhibition ofgastric acid secretion. Furthermore, they are shown to be associatedwith diseases, such as dwarfism, acromegaly, infantile chronic renalfailure, osteoporosis, breast cancer, prostate cancer, acute leukemia,large intestine cancer, uterine leiomyoma, liver cancer, type Idiabetes, type II diabetes, thrombosis, arteriosclerosis, hemolyticuremic syndrome, thrombotic thrombocytopenic purpa, sickle cell anemia,acute myocardial infarction, and diabetic angiopathy.

Therefore, proteins having the activity of IGFBP that belong to theIGFBP superfamily, genes encoding the proteins, antisense DNAs, andantibodies recognizing the proteins are considered to serve asmedicaments for detecting, treating, or preventing diseases accompanyingabnormal cell growth, diseases accompanying angiopathy, diseasesaccompanying abnormal bone metabolism, diseases accompanying disordersof IGF and growth hormone action, diseases accompanying abnormaldifferentiation or growth of smooth muscle cells, diseases accompanyingabnormal differentiation or growth of skeletal muscle cells, diseasesaccompanying abnormal gastric acid secretion, or inflammatory diseasesaccompanying abnormal lymphocyte invasion. Thus, great attention, asuseful targets for development of new drugs, has been paid on factorsbelonging to the IGFBP superfamily.

Furthermore, the possibility on the existence of novel factors belongingto the IGFBP superfamily was indicated. By obtaining a novel IGFBP gene,the function of this IGFBP can be estimated by comparing the amino acidsequence of the novel IGFBP with that of known IGFBPs, or by studyingthe expression distribution of transcription products of the IGFBP gene,to finally provide useful information for drug development. In addition,when a novel IGFBP gene is obtained, substances that suppress theexpression or function of the IGFBP can be screened. Compounds obtainedby this screening procedure are expected to serve as useful drugs.

BRIEF SUMMARY OF THE INVENTION Disclosure of the Invention

The present invention provides novel insulin-like growth factor bindingproteins, DNAs encoding the proteins, and antibodies recognizing theproteins, as well as detection methods, diagnostic agents, preventiveagents, and therapeutic agents for diseases associated with theproteins.

Upon extensive analysis to solve the above-mentioned objective, thepresent inventors succeeded in obtaining novel insulin-like growthfactor binding proteins belonging to the IGFBP family and DNAs encodingthe proteins, and accomplished the present invention.

More specifically, the present invention provides the following (1) to(60).

(1) An insulin-like growth factor binding protein, which comprises theamino acid sequence represented by SEQ ID NO: 1.

(2) An insulin-like growth factor binding protein, which comprises theamino acid sequence represented by SEQ ID NO: 2.

(3) A protein selected from the group of:

-   -   (A) a protein comprising an amino acid sequence, wherein one or        more amino acids have been deleted, substituted, or added in the        amino acid sequence represented by SEQ ID NO: 1, which protein        substantially has an identical activity to the protein according        to (1); and    -   (B) a protein comprising an amino acid sequence, wherein one or        more amino acids have been deleted, substituted, or added in the        amino acid sequence represented by SEQ ID NO: 2, which protein        substantially has an identical activity to the protein according        to (2).

(4) A protein selected from the group of:

-   -   (A) a protein comprising an amino acid sequence with 60% or        higher identity with the amino acid sequence represented by SEQ        ID NO: 1, wherein the protein substantially has an identical        activity to the protein according to (1); and    -   (B) a protein comprising an amino acid sequence with 60% or        higher identity with the amino acid sequence represented by SEQ        ID NO: 2, wherein the protein substantially has an identical        activity to the protein according to (2).

(5) A protein selected from the group of:

-   -   (A) a protein comprising an amino acid sequence that includes        the 75th to the 82nd amino acid residues of the amino acid        sequence represented by SEQ ID NO: 1, wherein the protein        substantially has an identical activity to the protein of (1);        and    -   (B) a protein comprising an amino acid sequence that includes        the 75th to the 82nd amino acid residues of the amino acid        sequence represented by SEQ ID NO: 2, wherein the protein        substantially has an identical activity to the protein of (2).

(6) A protein selected from the group of:

-   -   (A) a protein comprising an amino acid sequence wherein one or        more amino acids are deleted, substituted, or added in the amino        acid sequence according to (A) of (5), which protein        substantially has an identical activity to the protein according        to (1); and    -   (B) a protein comprising an amino acid sequence wherein one or        more amino acids are deleted, substituted, or added in the amino        acid sequence according to (B) of (5), which protein        substantially has an identical activity to the protein according        to (2).

(7) A protein selected from the group of:

-   -   (A) a protein comprising an amino acid sequence with 60% or        higher identity with the amino acid sequence according to (A) of        (5), which protein substantially has an identical activity to        the protein of (1); and    -   (B) a protein comprising an amino acid sequence with 60% or        higher identity with the amino acid sequence according to (B) of        (5), which protein substantially has an identical activity to        the protein of (2).

(8) A protein selected from the group of:

-   -   (A) the protein according to (A) of (5) comprising a partial        amino acid sequence that includes the 171st to the 304th amino        acid residues in the amino acid sequence represented by SEQ ID        NO: 1, which protein substantially has an identical activity to        the protein according to (1); and    -   (B) the protein according to (B) of (5) comprising a partial        amino acid sequence that includes the 171st to the 197th amino        acid residues in the amino acid sequence represented by SEQ ID        NO: 2, which protein substantially has an identical activity to        the protein according to (2).

(9) A protein selected from the group of:

-   -   (A) a protein comprising an amino acid sequence wherein one or        more amino acids are deleted, substituted, or added in the amino        acid sequence according to (A) of (8), which protein        substantially has an identical activity to the protein according        to (1); and    -   (B) a protein comprising an amino acid sequence wherein one or        more amino acids are deleted, substituted, or added in the amino        acid sequence according to (B) of (8), which protein        substantially has an identical activity to the protein according        to (2).

(10) A protein selected from the group consisting of:

-   -   (A) a protein comprising an amino acid sequence with 60% or        higher identity with the amino acid sequence according to (A) of        (8), which protein substantially has an identical activity to        the protein according to (1); and    -   (B) a protein comprising an amino acid sequence with 60% or        higher identity with the amino acid sequence according to (B) of        (8), which protein substantially has an identical activity to        the protein according to (2).

(11) A polypeptide selected from the group of:

-   -   (A) a polypeptide comprising an amino acid sequence that        includes at least 5 or more continuous amino acids in the amino        acid sequence represented by SEQ ID NO: 1; and    -   (B) a polypeptide comprising an amino acid sequence that        includes at least 5 or more continuous amino acids in the amino        acid sequence represented by SEQ ID NO: 2.

(12) A polypeptide selected from the group of:

-   -   (A) a polypeptide comprising an amino acid sequence that        includes at least 5 or more continuous amino acids of the 171st        to the 304th amino acid residues in the amino acid sequence        represented by SEQ ID NO: 1; and    -   (B) a polypeptide comprising an amino acid sequence that        includes at least 5 or more continuous amino acids of the 171st        to the 197th amino acid residues in the amino acid sequence        represented by SEQ ID NO: 2.

(13) A DNA encoding the protein or the polypeptide according to any oneof (1) to (12).

(14) A DNA containing the coding region of the nucleotide sequencerepresented by SEQ ID NO: 3.

(15) A DNA containing the coding region of the nucleotide sequencerepresented by SEQ ID NO: 4.

(16) A DNA selected from the group of:

-   -   (A) a DNA that hybridizes under stringent conditions to the DNA        encoding the protein according to (1), the DNA encoding the        protein or the polypeptide of (A) according to any one of (3) to        (12), or the DNA comprising the coding region of the nucleotide        sequence represented by SEQ ID NO: 3, wherein the DNA encodes a        protein that substantially has an identical activity to the        protein according to (1); and    -   (B) a DNA that hybridizes under stringent conditions to the DNA        encoding the protein according to (2), the DNA encoding the        protein or the polypeptide of (B) according to any one of (3) to        (12), or the DNA comprising the coding region of the nucleotide        sequence represented by SEQ ID NO: 4, wherein the DNA encodes a        protein that substantially has an identical activity to the        protein according to (2).

(17) A recombinant DNA obtained by ligating the DNA according to any oneof (13) to (16) with a vector.

(18) A transformant obtained by introducing the recombinant DNAaccording to (13) into a host cell.

(19) The transformant according to (18), wherein the host cell is a cellselected from the group consisting of bacteria, yeast, insect cell,plant cell, and animal cell.

(20) A transformant FERM BP-7181 containing the DNA according to (14).

(21) A transformant FERM BP-7180 containing the DNA according to (15).

(22) A process for producing the protein or the polypeptide according toany one of (1) to (12), which comprises the steps of: culturing atransformant according to any one of (18) to (21) in a media so as toproduce and accumulate the protein or the polypeptide according to anyone of (1) to (12) in a culture; and recovering the protein or thepolypeptide from the culture.

(23) An antibody recognizing the protein according to any one of (1) to(10).

(24) An antibody recognizing the protein according to any one of (1) or(A) of (3) to (10), but which does not recognize the protein accordingto any one of (2) or (B) of (3) to (10).

(25) An antibody recognizing the protein according to any one of (2) or(B) of (3) to (10), but which does not recognize the protein accordingto any one of (1) or (A) of (3) to (10).

(26) A method for producing an antibody recognizing the proteinaccording to any one of (1) or (A) of (3) to (10), wherein thepolypeptide according to (A) of (11) or (A) of (12) is used as theantigen.

(27) A method for producing an antibody recognizing the proteinaccording to any one of (2) or (B) of (3) to (10), wherein thepolypeptide according to (B) of (11) or (B) of (12) is used as theantigen.

(28) An antibody obtained by the method according to (26), wherein theantibody specifically recognizes the protein according to any one of (1)or (A) of (3) to (10).

(29) An antibody obtained by the method according to (27), wherein theantibody specifically recognizes the protein according to any one of (2)or (B) of (3) to (10).

(30) A monoclonal antibody produced by a hybridoma with the accessionnumber FERM BP-7603.

(31) A monoclonal antibody produced by a hybridoma with the accessionnumber FERM BP-7604.

(32) A method for immunologically detecting or quantifying the proteinaccording to any one of (1) to (10), wherein the antibody according toany one of (23) to (25) or (28) to (31) is used.

(33) An oligonucleotide having a sequence comprising 5 to 60 continuousnucleotides of the nucleotide sequence of the DNA according to any oneof (13) to (16), an oligonucleotide having a sequence complementary tosaid oligonucleotide, or derivatives thereof.

(34) A method for detecting or quantifying the expression of a geneencoding the protein according to any one of (1) to (10), whereinhybridization is conducted using the DNA according to any one of (13) to(16), or the oligonucleotide or the oligonucleotide derivativesaccording to (33) as a probe.

(35) A method for detecting or quantifying the expression of a geneencoding the protein according to any one of (1) to (10), whereinpolymerase chain reaction is conducted using the oligonucleotide or theoligonucleotide derivative according to (33) as a primer.

(36) A method for detecting the mutation of a gene encoding the proteinaccording to any one of (1) to (10), wherein the hybridization isconducted using the DNA according to any one of (13) to (16), or theoligonucleotide or the oligonucleotide derivative according to (33).

(37) A method for detecting the mutation of a gene encoding the proteinaccording to any one of (1) to (10), wherein polymerase chain reactionis conducted using the oligonucleotide or the oligonucleotide derivativeaccording to (33).

(38) A method for detecting a disease selected from the group of:

-   -   (A) a method for detecting a disease, wherein the mutation is        detected or the expression level is measured for the DNA        encoding the protein according to any one of (1) to (10), and        then compared with that of a healthy individual; and    -   (B) a method for detecting a disease, wherein the mutation is        detected or the expression level is measured for the protein        according to any one of (1) to (10), and then compared with that        of a healthy individual.

(39) A method for detecting a disease selected from the group of:

-   -   (A) a method for detecting a disease, wherein the mutation or        expression level of the DNA encoding the protein according to        any one of (1) or (A) of (3) to (10) is compared with that of        the DNA encoding the protein according to any one of (2) or (B)        of (3) to (10); and    -   (B) a method for detecting a disease, wherein the mutation or        expression level of the protein according to any one of (1)        or (A) of (3) to (10) is compared with that of the protein        according to any one of (2) or (B) of (3) to (10).

(40) The detection method according to (38) or (39), wherein the diseaseis selected from the group consisting of: diseases accompanying abnormalcell growth, diseases accompanying angiopathy, diseases accompanyingabnormal bone metabolism, diseases accompanying disorders ofinsulin-like growth factors or growth hormone action, diseasesaccompanying abnormal differentiation or growth of smooth muscle cells,diseases accompanying abnormal differentiation or growth of skeletalmuscle cells, diseases accompanying abnormal gastric acid secretion, andinflammatory diseases accompanying abnormal lymphocyte invasion.

(41) The detection method according to (40), wherein the diseaseaccompanying abnormal cell growth is selected from the group consistingof: acute myelogenous leukemia, breast cancer, prostate cancer, coloncancer, hepatic cancer, myeloma, uterine leiomyoma, malignant tumor, andsolid tumor; the disease accompanying angiopathy is selected from thegroup consisting of: myocardial infarction, cerebral infarction,peripheral vascular atresia, angina pectoris, hypertension,hyperlipidemia, diabetes, diabetic retinopathy, glomerulonephritis,arteriosclerosis, thrombosis, hemolytic uremic syndrome, thromboticthrombocytopenic purpa, ischemic heart disease, ischemic encephalopathy,heart failure, hemostasis, and choroid circulatory dysfunction; thedisease accompanying abnormal bone metabolism is osteoporosis; thedisease accompanying disorders of insulin-like growth factors or growthhormone action is selected from the group consisting of dwarfism,acromegaly, and infantile chronic renal failure; the diseaseaccompanying abnormal differentiation or growth of smooth muscle cellsis selected from the group consisting of: arteriosclerosis, bronchialdisease, and restenosis; the disease accompanying abnormaldifferentiation or growth of skeletal muscle cells is myasthenia gravis;the disease accompanying abnormal gastric acid secretion is gastriculcer; and the inflammatory disease accompanying abnormal lymphocyteinvasion is selected from the group consisting of: microbial infection,chronic hepatitis B, chronic rheumatoid arthritis, sepsis, graft versushost disease, insulin dependent diabetes mellitus, nephritis, traumaticbrain damage, inflammatory enteropathy, allergy, atopy, asthma,pollinosis, airway hypersensitivity, and autoimmune disease.

(42) The detection method according to any one of (38) to (41), whereinthe detection method is conducted by the method selected from the groupof:

-   -   (A) the detection method of (36) or (37); and    -   (B) the detection or quantification method of (32), (34), or        (35).

(43) A medicament containing the protein or the polypeptide according toany one of (1) to (12).

(44) A medicament, which contains the DNA according to any one of (13)to (16), or the oligonucleotide or the oligonucleotide derivativeaccording to (33).

(45) The medicament according to (44), wherein the medicament is avector for genetic prevention or a vector for gene therapy.

(46) A medicament containing the antibody according to any one of (23)to (25) and (28) to (31).

(47) The medicament according to any one of (43) to (46), wherein themedicament is a diagnostic agent, a preventive agent, or a therapeuticagents for diseases accompanying abnormal cell growth, diseasesaccompanying angiopathy, diseases accompanying abnormal bone metabolism,diseases accompanying disorders of insulin-like growth factors or growthhormone action, diseases accompanying abnormal differentiation or growthof smooth muscle cells, diseases accompanying abnormal differentiationor growth of skeletal muscle cells, diseases accompanying abnormalgastric acid secretion, or inflammatory diseases accompanying abnormallymphocyte invasion.

(48) The medicament according to (47), wherein the disease accompanyingabnormal cell growth is acute myelogenous leukemia, breast cancer,prostate cancer, colon cancer, hepatic cancer, myeloma, uterineleiomyoma, malignant tumor, or solid tumor; the disease accompanyingangiopathy is myocardial infarction, cerebral infarction, peripheralvascular atresia, angina pectoris, hypertension, hyperlipidemia,diabetes, diabetic retinopathy, glomerulonephritis, arteriosclerosis,thrombosis, hemolytic uremic syndrome, thrombotic thrombocytopenicpurpa, ischemic heart disease, ischemic encephalopathy, heart failure,hemostasis, or choroid circulatory dysfunction; the disease accompanyingabnormal bone metabolism is osteoporosis; the disease accompanyingdisorders of insulin-like growth factors or growth hormone action isdwarfism, acromegaly, or infantile chronic renal failure; the diseaseaccompanying abnormal differentiation or growth of smooth muscle cellsis arteriosclerosis, bronchial disease, or restenosis; the diseaseaccompanying abnormal differentiation or growth of skeletal muscle cellsis myasthenia gravis; the disease accompanying abnormal gastric acidsecretion is gastric ulcer; and the inflammatory disease accompanyingabnormal lymphocyte invasion is microbial infection, chronic hepatitisB, chronic rheumatoid arthritis, sepsis, graft versus host disease,insulin dependent diabetes mellitus, nephritis, traumatic brain damage,inflammatory enteropathy, allergy, atopy, asthma, pollinosis, airwayhypersensitivity, or autoimmune disease.

(49) A method of screening for a compound that regulates the expressionlevel of the protein according to any one of (1) to (10), whichcomprises the steps of: (a) comparing (i) the expression level of theprotein according to any one of (1) to (10) in a cell expressing theprotein, and (ii) the expression level of the protein in the cellcontacted with a test sample; and (b) selecting the compound thatregulates the expression level of the protein according to any one of(1) to (10) from the test samples.

(50) A method of screening for a compound that regulates the function ofthe protein according to any one of (1) to (10), which comprises thesteps of: (a) comparing the function of (i) a cell expressing theprotein according to any one of (1) to (10) and the function of (ii) thecell contacted to a test sample; and (b) selecting the compound thatregulates the function of the protein according to any one of (1) to(10) from the test sample.

(51) A method of screening for a compound that regulates the expressionof a gene encoding the protein according to any one of (1) to (10),which comprises the steps of: (a) contacting a transformant transformedwith a plasmid containing a DNA wherein a reporter gene is connecteddownstream of a region regulating the transcription of a gene encodingthe protein according to any one of (1) to (10) with a test sample; and(b) selecting the compound that regulates the expression of the geneencoding the protein according to any one of (1) to (10) from the testsample.

(52) A method of screening for a compound that regulates the splicing ofa gene encoding the protein according to any one of (1) to (10) from atest sample using the cell expressing the protein according to any oneof (1) to (10), which comprises the steps of: (a) contacting the cellexpressing said protein with the test sample; and (b) comparing (i) theexpression level of the protein according to any one of (1) or (A) of(3) to (10) in said cell, and (ii) the expression level of the proteinaccording to any one of (2) or (B) of (3) to (10) in said cell.

(53) A compound or pharmacologically acceptable salt thereof, which isobtainable by the screening method according to any one of (49) to (52).

(54) A medicament containing the compound or pharmacologicallyacceptable salt thereof of (53).

(55) The medicament according to (54), wherein the medicament is apreventive agent or a therapeutic agents for diseases accompanyingabnormal cell growth, diseases accompanying angiopathy, diseasesaccompanying abnormal bone metabolism, diseases accompanying disordersof insulin-like growth factors or growth hormone action, diseasesaccompanying abnormal differentiation or growth of smooth muscle cells,diseases accompanying abnormal differentiation or growth of skeletalmuscle cells, diseases accompanying abnormal gastric acid secretion, orinflammatory diseases accompanying abnormal lymphocyte invasion.

(56) The medicament according to (55), wherein the disease accompanyingabnormal cell growth is acute myelogenous leukemia, breast cancer,prostate cancer, colon cancer, hepatic cancer, myeloma, uterineleiomyoma, malignant tumor, or solid tumor; the disease accompanyingangiopathy is myocardial infarction, cerebral infarction, peripheralvascular atresia, angina pectoris, hypertension, hyperlipidemia,diabetes, diabetic retinopathy, glomerulonephritis, arteriosclerosis,thrombosis, hemolytic uremic syndrome, thrombotic thrombocytopenicpurpa, ischemic heart disease, ischemic encephalopathy, heart failure,hemostasis, or choroid circulatory dysfunction; the disease accompanyingabnormal bone metabolism is osteoporosis; the disease accompanyingdisorders of insulin-like growth factors or growth hormone action isdwarfism, acromegaly, or infantile chronic renal failure; the diseaseaccompanying abnormal differentiation or growth of smooth muscle cellsis arteriosclerosis, bronchial disease, or restenosis; the diseaseaccompanying abnormal differentiation or growth of skeletal muscle cellsis myasthenia gravis; the disease accompanying abnormal gastric acidsecretion is gastric ulcer; and the inflammatory disease accompanyingabnormal lymphocyte invasion is microbial infection, chronic hepatitisB, chronic rheumatoid arthritis, sepsis, graft versus host disease,insulin dependent diabetes mellitus, nephritis, traumatic brain damage,inflammatory enteropathy, allergy, atopy, asthma, pollinosis, airwayhypersensitivity, or autoimmune disease.

(57) A method of screening for a substance that specifically binds tothe protein or polypeptide according to any one of (1) to (12), whichcomprises the steps of: (a) contacting the protein or polypeptideaccording to any one of (1) to (12) with a test sample; and (b)selecting the substance that specifically binds to the protein orpolypeptide from the test sample.

(58) A substance that specifically binds to the protein or polypeptideaccording to any one of (1) to (12), which is obtained by the screeningmethod of (57).

(59) A non-human knockout animal, wherein the expression of a geneencoding the protein according to any one of (1) to (10) is decreased orcompletely inhibited.

(60) A non-human knockout animal, wherein the function of the proteinaccording to any one of (1) to (10) is decreased or completelyinhibited.

Proteins and polypeptides of the present invention comprise thefollowing:

-   -   (a) an insulin-like growth factor binding protein that comprises        the amino acid sequence shown in SEQ ID NO: 1;    -   (b) an insulin-like growth factor binding protein that comprises        the amino acid sequence shown in SEQ ID NO: 2;    -   (c) a protein comprising an amino acid sequence, wherein one or        more amino acids have been deleted, substituted, or added in the        amino acid sequence shown in SEQ ID NO: 1, and which        substantially has an identical activity to the protein of (a);    -   (d) a protein comprising an amino acid sequence, wherein one or        more amino acids have been deleted, substituted, or added in the        amino acid sequence shown in SEQ ID NO: 2, and which        substantially has an identical activity to the protein of (b);    -   (e) a protein comprising an amino acid sequence having 60% or        higher identity with the amino acid sequence shown in SEQ ID NO:        1, wherein the protein substantially has an identical activity        to the protein of (a);    -   (f) a protein comprising an amino acid sequence having 60% or        higher identity with the amino acid sequence shown in SEQ ID NO:        2, wherein the protein substantially has an identical activity        to the protein of (b);    -   (g) a protein comprising an amino acid sequence that includes        the 75th to the 82nd amino acid residues of the amino acid        sequence shown in SEQ ID NO: 1, wherein the protein        substantially has an identical activity to the protein of (a);    -   (h) a protein comprising an amino acid sequence that includes        the 75th to the 82nd amino acid residues of the amino acid        sequence shown in SEQ ID NO: 2, wherein the protein        substantially has an identical activity to the protein of (b);    -   (i) a protein comprising an amino acid sequence, wherein one or        more amino acids are deleted, substituted, or added in the amino        acid sequence of (g), which substantially has an identical        activity as the protein of (a);    -   (j) a protein comprising an amino acid sequence, wherein one or        more amino acids are deleted, substituted, or added in the amino        acid sequence of (h), which substantially has an identical        activity to the protein of (b);    -   (k) a protein comprising an amino acid sequence having 60% or        higher identity with the amino acid sequence of (g), wherein the        protein substantially has an identical activity to the protein        of (a);    -   (l) a protein comprising an amino acid sequence having 60% or        higher identity with the amino acid sequence of (h), wherein the        protein substantially has an identical activity to the protein        of (b);    -   (m) the protein comprising a partial amino acid sequence that        includes the 171st to the 304th amino acid residues of the amino        acid sequence shown in SEQ ID NO: 1, wherein the protein        substantially has an identical activity to the protein of (a);    -   (n) the protein comprising a partial amino acid sequence that        includes the 171st to the 197th amino acid residues of the amino        acid sequence shown in SEQ ID NO: 2, wherein the protein        substantially has an identical activity to the protein of (b);    -   (o) a protein comprising an amino acid sequence, wherein one or        more amino acids are deleted, substituted, or added in the amino        acid sequence of (m), which substantially has an identical        activity to the protein of (a);    -   (p) a protein comprising an amino acid sequence, wherein one or        more amino acids are deleted, substituted, or added in the amino        acid sequence of (n), which substantially has an identical        activity to the protein of (b);    -   (q) a protein comprising an amino acid sequence having 60% or        higher identity with the amino acid sequence of (m), wherein the        protein substantially has an identical activity to the protein        of (a);    -   (r) a protein comprising an amino acid sequence having 60% or        higher identity with the amino acid sequence of (n), wherein the        protein substantially has an identical activity to the protein        of (b);    -   (s) a polypeptide comprising an amino acid sequence that        includes at least 5 or more continuous amino acids in the amino        acid sequence shown in SEQ ID NO: 1;    -   (t) a polypeptide comprising an amino acid sequence that        includes at least 5 or more continuous amino acids in the amino        acid sequence shown in SEQ ID NO: 2;    -   (u) a polypeptide comprising an amino acid sequence that        includes at least 5 or more continuous amino acids of the 171st        to the 304th amino acid residues in the amino acid sequence        shown in SEQ ID NO: 1; and    -   (v) a polypeptide comprising an amino acid sequence that        includes at least 5 or more continuous amino acids of the 171st        to the 197th amino acid residues in the amino acid sequence        shown in SEQ ID NO: 2.

A factor belonging to the insulin-like growth factor binding proteinsuperfamily has the affinity to IGF or insulin and an insulin-likegrowth factor binding motif (IGFBP motif). The motif is conserved in theN-terminal region of proteins belonging to the insulin-like growthfactor binding protein superfamily, and refers to a region of theprotein consisting of the amino acid sequence represented byGly-Cys-Gly-Cys-Cys-X-X-Cys (SEQ ID NO:26) (X refers to an arbitraryamino acid).

At least 10 cysteine residues are conserved, mainly as the IGFBP motif,in proteins belonging to the insulin-like growth factor binding protein,and these proteins show an affinity to IGF or insulin via the IGFBPmotif region. The amino acid sequence represented by the 75th to the82nd amino acid in the amino acid sequence shown in SEQ ID NO: 1, andthe 75th to the 82nd amino acid residues in the amino acid sequenceshown in SEQ ID NO: 2 are the IGFBP motifs.

Examples of the above-mentioned proteins having substantially identicalactivity to the protein having the amino acid sequence shown in SEQ IDNO: 1 or SEQ ID NO: 2 include those which bind to the same protein, suchas integrin, IGF, insulin, or receptors, as the protein having the aminoacid sequence shown in SEQ ID NO: 1 or SEQ ID NO: 2. The term“substantially identical” indicates that the activity is qualitativelyidentical. Therefore, quantitative factors, such as the degree ofbinding activity and molecular weight of the protein, may be different.

A protein which comprises an amino acid sequence wherein one or moreamino acids are deleted, substituted, or added in the amino acidsequence shown in SEQ ID NO: 1 coding for the protein of the presentinvention, and which substantially has an identical activity to theprotein of SEQ ID NO: 1, or a protein which comprises an amino acidsequence wherein one or more amino acids are deleted, substituted, oradded in the amino acid sequence shown in SEQ ID NO: 2 coding for theprotein of the present invention, and which substantially has anidentical activity to the protein of SEQ ID NO: 2 can be obtained bysite-directed mutagenesis of a DNA coding for a protein having an aminoacid sequence shown in, for example, SEQ ID NO: 1 or 2, using the methodfor site-directed mutagenesis described, for example, in MolecularCloning, A Laboratory Manual, Second Edition, Cold Spring HarborLaboratory Press (1989) (hereinafter, abbreviated as Molecular CloningSecond Edition); Current Protocols in Molecular Biology, John Wiley &Sons (1987-1997) (hereinafter, abbreviated as Current Protocols inMolecular Biology); Nucleic Acids Research, 10, 6487 (1982); Proc. Natl.Acad. Sci., USA, 79, 6409 (1982); Gene, 34, 315 (1985); Nucleic AcidsResearch, 13, 4431 (1985); and Proc. Natl. Acad. Sci., USA, 82, 488(1985). The numbers of amino acid(s) which is/are deleted, substituted,or added is more than 1 and there is no particular limitation on thenumbers so long as they can be deleted, substituted or added byconventional methods, such as site-directed mutagenesis described above,for example, one to several tens amino acid(s), preferably one to twentyamino acid(s), more preferably one to ten amino acid(s), still morepreferably one to five amino acid(s). Furthermore, the proteins of thepresent invention include not only such artificially prepared variantsbut also naturally-occurring variants.

So that a protein substantially has an identical activity to the proteinconsisting of the amino acid sequence shown in SEQ ID NO: 1 or 2, it ispreferred that the protein consists of an amino acid sequence having atleast 60% or more, preferably 70% or more, more preferably 80% or more,still more preferably 90% or more, particularly more preferably 95% ormore, and most preferably 97% or more identity to the amino acidsequence shown in SEQ ID NO: 1 or 2.

The identity of amino acid sequences or nucleotide sequences can bedetermined with the BLAST algorithms of Karlin and Altschul (Proc. Natl.Acad. Sci. USA, 90, 5873-5877 (1993)) and FASTA (Methods Enzymol., 183,63 (1990)). Based on the BLAST algorithm, the programs, BLASTN andBLASTX, have been developed (J. Mol. Biol., 215, 403 (1990)). Whennucleotide sequences are analyzed by BLASTN based on BLAST, theparameters are set, for example, as follows: score=100; andwordlength=12. Alternatively, when amino acid sequences are analyzed byBLASTX based on BLAST, the parameters are set, for example, as follows:score=50; and wordlength=3. When BLAST and the Gapped BLAST program areused for the analysis, the default parameters are used in each program.The specific techniques used in these analysis methods are already known(www.ncbi.nlm.nih.gov.).

The proteins of the present invention are proteins that have a partialamino acid sequence of the above-mentioned protein of the presentinvention, and includes proteins that substantially have identicalactivity to the protein having the amino acid sequence shown in SEQ IDNO: 1 or 2.

So that a protein substantially has an identical activity to the proteinhaving the amino acid sequence shown in SEQ ID NO: 1 or 2, it ispreferred that the protein comprises a partial amino acid sequencecontaining the 75th to the 82nd amino acid residues of the amino acidsequence shown in SEQ ID NO: 1, or the 75th to the 82nd amino acidresidues in the amino acid sequence shown in SEQ ID NO: 2.

A protein having an amino acid sequence wherein one or more amino acidsin the amino acid of such protein are deleted, substituted, or added, isalso included in the protein of the present invention. An example of theprotein includes the above-mentioned protein wherein a number of aminoacids that can be deleted, substituted, or added by well-known sitedirected mutagenesis method are modified and which substantially has anidentical activity to the protein having the amino acid sequence shownin SEQ ID NO: 1 or 2.

An example of a protein of the present invention comprising an aminoacid sequence wherein one or more amino acids are deleted in the aminoacid sequence shown in SEQ ID NO: 1 or 2 which substantially has anidentical activity to a protein consisting of the amino acid sequenceshown in SEQ ID NO: 1 or 2, includes a protein wherein the signalpeptide has been removed.

Furthermore, proteins having 60% or higher identity with theabove-mentioned protein of (g), (h), (m), or (n), which substantiallyhave an identical activity to the protein having the amino acid sequenceshown in SEQ ID NO: 1 or 2, are also included in the proteins of thepresent invention. So that a protein substantially has an identicalactivity to a protein having the amino acid sequence shown in SEQ ID NO:1 or 2, its identity with the amino acid sequence of the protein of (g),(h), (m), or (n) is at least 60% or higher, preferably 70% or higher,more preferably 80% or higher, even more preferably 90% or higher,especially preferably 95% or higher, and most preferably 97% or higher,when the identity is calculated by, for example, analysis software, suchas BLAST described above.

There are no limitations on the polypeptides of the present invention solong as the polypeptides can be used as antigenic polypeptides forproducing antibodies that recognize the above-mentioned proteins of thepresent invention. However, specific examples include polypeptidescomprising 5 or more continuous amino acids of the amino acid sequenceshown in SEQ ID NO: 1 or 2, preferably 10 or more, and more preferably15 or more. For producing antibodies that can distinguish proteinsconsisting of the amino acid sequence shown in SEQ ID NO: 1 and SEQ IDNO: 2, particularly useful polypeptides include those consisting of 5 ormore, preferably 10 or more, and more preferably 15 or more continuousamino acids of the 171st to the 304th amino acid residues in the aminoacid sequence shown in SEQ ID NO: 1 or the 171st to the 197th amino acidresidues in the amino acid sequence shown in SEQ ID NO: 2.

The DNA of the present invention includes the following:

-   -   (1) a DNA having the nucleotide sequence shown in SEQ ID NO: 3;    -   (2) a DNA having the nucleotide sequence shown in SEQ ID NO: 4;    -   (3) a DNA encoding the protein of the present invention        according to (a), (c), (e), (g), (i), (k), (m), (o), or (q)        defined above;    -   (4) a DNA encoding the protein of the present invention        according to (b), (d), (f), (h), (o), (l), (n), (p), or (r)        defined above;    -   (5) a DNA that hybridizes under stringent conditions to the DNA        of (3) or a DNA comprising the nucleotide sequence shown in SEQ        ID NO: 3 from the 177th to the 1088th bases, which encodes a        protein that substantially has an identical activity to the        protein having the amino acid sequence shown in SEQ ID NO: 1;    -   (6) a DNA that hybridizes under stringent conditions to the DNA        of (4) or a DNA comprising a nucleotide sequence shown in SEQ ID        NO: 4 from the 926th to the 1516th bases, which encodes a        protein that substantially has an identical activity to the        protein having the amino acid sequence shown in SEQ ID NO: 2;    -   (7) a DNA encoding the polypeptide of (s) or (u) defined above;        and    -   (8) a DNA encoding the polypeptide of (t) or (v) defined above.

The “DNA that hybridizes under stringent conditions” refers to a DNAthat can be obtained by colony hybridization, plaque hybridization,Southern blot hybridization, or the like, using the DNA of the presentinvention, such as those having the nucleotide sequence shown in SEQ IDNO: 3 or 4, or fragments thereof as a probe. Specifically, such DNAincludes a DNA that can be identified by carrying out hybridization at65° C. in the presence of 0.7 to 1.0 mol/L NaCl using a filter, on whicha DNA derived from a colony or plaque is immobilized; and then washingthe filter under a condition of 65° C. with 0.1× to 2×SSC solution(1×SSC solution consists of 150 mmol/L NaCl, 15 mmol/L sodium citrate).The hybridization can be performed according to the method described in,for example, “Molecular Cloning, Second Edition”; “Current Protocols inMolecular Biology”; and “DNA Cloning 1: Core Techniques, A PracticalApproach, Second Edition”, Oxford University Press (1995). Specificexamples of hybridizing DNAs include a DNA exhibiting at least 60% orhigher, preferably 70% or higher, more preferably 80% or higher, stillmore preferably 90% or higher, particularly preferably 95% or higher,most preferably 98% or higher identity with the nucleotide sequenceshown in SEQ ID NO: 3 or 4, when the identity is computed by, forexample, analysis software such as BLAST described above.

The above-mentioned DNAs of (7) and (8) are DNAs encoding thepolypeptides of the present invention, and thus can be used forproducing the polypeptides of the present invention, and for detectingthe expression or measuring the expression level of DNAs containing thecoding region of the nucleotide sequence shown in SEQ ID NO: 3 or 4. Inparticular, DNAs encoding the polypeptides of the above-mentioned (u)and (v) are useful for diagnosis of a disease characterized by anabnormal expression ratio of the protein of SEQ ID NO: 1 to the proteinof SEQ ID NO: 2, wherein the expression levels of a DNA containing thecoding region of the nucleotide sequence shown in SEQ ID NO: 3 and a DNAcontaining the coding region of the nucleotide sequence shown in SEQ IDNO: 4 are compared.

DETAILED DESCRIPTION OF THE INVENTION

The present invention is described in detail below.

1. Preparation of DNAs of the Present Invention

A DNA of the present invention can be prepared by isolating humantissue-derived mRNAs, for example, human small intestine-derived mRNA;preparing their cDNA library; and then screening the cDNA library toobtain a clone of interest.

Commercially available human small intestine mRNAs (for example, thosefrom Clontech) may be used, or human small intestine mRNAs may beprepared from human small intestine tissues as described below. In thelatter case, first, total RNA is prepared from small intestine tissue,and then mRNA can be isolated from the total RNA.

Examples of methods for preparing total RNA from small intestine tissueinclude the guanidine thiocyanate-cesium trifluoroacetate method(Methods in Enzymology, 154, 3 (1987)), and the acid guanidinethiocyanate-phenol-chloroform (AGPC) method (Analytical Biochemistry,162, 156 (1987); Experimental Medicine, 9, 1937 (1991)). Examples ofmethods for preparing mRNA as poly(A)⁺ RNA from total RNA include theoligo(dT) immobilized cellulose column method (Molecular Cloning, 2ndedition), and the like. Alternatively, mRNA can be prepared using a kitsuch as Fast Track mRNA Isolation Kit (Invitrogen) and Quick Prep mRNAPurification Kit (Pharmacia).

A cDNA library is constructed from the prepared human small intestinetissue mRNA. Examples of methods for constructing the cDNA libraryinclude methods described in Molecular Cloning, second edition, CurrentProtocols in Molecular Biology, and the like, or methods usingcommercially available kits, for example, SuperScript Plasmid System forcDNA Synthesis and Plasmid Cloning (Life Technologies), and ZAP-cDNASynthesis Kit (STRATAGENE).

Phage vectors, plasmid vectors, and such can be used as the cloningvector for constructing the cDNA library so long as they canself-replicate in Escherichia coli K12 cell line. Specific examplesinclude: ZAP Express (STRATAGENE; Strategies, 5, 58 (1992)); pBluescriptII SK(+) (Nucleic Acids Research, 17, 9494 (1989)); Lambda ZAP II(STRATAGENE); λgt10; λgt11 (DNA cloning, A Practical Approach, 1, 49(1985)); λTriplEx (Clontech); λExCell (Pharmacia); pT7T318U (Pharmacia);pcD2 (Mol. Cell. Biol., 3, 280 (1993)); pUC18 (Gene, 33, 103 (1985));and the like.

Any host microorganism can be used, as long as it belongs to the genusEscherichia, in particular, to Escherichia coli. Specifically,Escherichia coli XL1-Blue MRF′ (STRATAGENE, Strategies, 5, 81 (1992)),Escherichia coli C600 (Genetics, 39, 440 (1954)), Escherichia coli Y1088(Science, 222, 778 (1983)), Escherichia coli Y1090 (Science, 222, 778(1983)), Escherichia coli NM522 (J. Mol. Biol., 166, 1 (1983)),Escherichia coli K802 (J. Mol. Biol., 16, 118 (1966)), Escherichia coliJM105 (Gene, 38, 275 (1985)), and the like are used.

The cDNA library may be used directly for the following analyses, or acDNA library prepared according to the oligo-capping method developed byKanno et al. (Gene, 138, 171 (1994); Gene, 200, 149 (1997); Protein,Nucleic Acid, and Enzyme, 41, 603 (1996); Experimental Medicine, 11,2491 (1993); “cDNA Cloning”, Yodosha (1996); “Idenshi Raiburari noSakuseiho (Method for Producing Gene Libraries)”, Yodosha (1994)) toefficiently obtain full-length cDNA while lowering the proportion ofcDNAs with incomplete-length may be used.

The nucleotide sequence of a DNA is determined by isolating each clonefrom the prepared cDNA library; and analyzing the nucleotide sequence ofthe cDNA for each clone from the end by ordinarily used nucleotidesequence analysis methods, for example, the dideoxy method of Sanger etal. (Proc. Natl. Acad. Sci. USA, 74, 5463 (1977)), or using nucleotidesequence analyzer, such as ABIPRISM377 DNA sequencer (PE Biosystems).

Whether respective cDNA nucleotide sequences have a novel sequence ornot can be determined by confirming the absence of a nucleotide sequencewith such an obvious homology that indicates a completely matchingnucleotide sequence to a gene existing in the database by searchingnucleotide sequence database, such as GenBank, EMBL, and DDBJ, using ahomology search program, such as BLAST.

An example of nucleotide sequences of cDNAs containing a novel sequenceobtainable by the above-mentioned method includes the nucleotidesequence shown in SEQ ID NOS: 3 and 4.

The protein (the protein consisting of the amino acid sequence shown inSEQ ID NO: 1) that is obtained by translating the DNA consisting of thenucleotide sequence shown in SEQ ID NO: 3 shows 38% homology to humanIGFBP-7, a protein belonging to the IGFBP superfamily, according to thehomology analysis using BLAST2 (Nuc. Acid. Res., 25, 3389 (1997)).

The protein (the protein consisting of the amino acid sequence shown inSEQ ID NO: 2) that is obtained by translating the DNA consisting of thenucleotide sequence shown in SEQ ID NO: 4 shows 35% homology to humanIGFBP-7, a protein belonging to the IGFBP superfamily, according to thehomology analysis using BLAST2.

Positions of at least 10 cysteine residues, mainly in the insulin-likegrowth factor binding motif region and the IGFBP motif that areimportant for binding with IGF or insulin, are known to be conserved inthe IGFBP superfamily. Similarly, in the proteins consisting of theamino acid sequences shown in SEQ ID NO: 1 and 2, an amino acid sequenceconsisting of Ala-Gly-Gly-Cys-Cys-Trp-Glu-Cys (SEQ ID NO:27) that isextremely similar to the amino acid sequence ofGly-Cys-Gly-Cys-Cys-X-X-Cys (SEQ ID NO:26) (X indicates an arbitraryamino acid), a representative insulin-like growth factor binding motif,exists at the region corresponding to the insulin-like growth factorbinding motif; and 10 cysteine residues, mainly in this motif region,exist at positions that are conserved among factors belonging to theIGFBP superfamily. Therefore, it is clear that proteins consisting ofthe amino acid sequences shown in SEQ ID NO: 1 and SEQ ID NO: 2 have theactivity of a protein belonging to the IGFBP superfamily.

Proteins belonging to the IGFBP superfamily are reported to beassociated with diseases including diseases accompanying abnormal cellgrowth, such as acute myelogenous leukemia, breast cancer, prostatecancer, colon cancer, hepatic cancer, myeloma, uterine leiomyoma,malignant tumor, and solid tumor; diseases accompanying angiopathy, suchas myocardial infarction, cerebral infarction, peripheral vascularatresia, angina pectoris, hypertension, hyperlipidemia, diabetes,diabetic retinopathy, glomerulonephritis, arteriosclerosis, thrombosis,hemolytic uremic syndrome, thrombotic thrombocytopenic purpa, ischemicheart disease, ischemic encephalopathy, heart failure, hemostasis, andchoroid circulatory dysfunction; diseases accompanying abnormal bonemetabolism, such as osteoporosis; diseases accompanying disorders ofinsulin-like growth factors or growth hormone action, such as dwarfism,acromegaly, and infantile chronic renal failure; diseases accompanyingabnormal differentiation or growth of smooth muscle cells, such asarteriosclerosis, bronchial disease, and restenosis; diseasesaccompanying abnormal differentiation or growth of skeletal musclecells, such as myasthenia gravis; diseases accompanying abnormal gastricacid secretion, such as gastric ulcer; and inflammatory diseasesaccompanying abnormal lymphocyte invasion, such as microbial infection,chronic hepatitis B, chronic rheumatoid arthritis, sepsis, graft versushost disease, insulin dependent diabetes mellitus, nephritis, traumaticbrain damage, inflammatory enteropathy, allergy, atopy, asthma,pollinosis, airway hypersensitivity, and autoimmune disease. Therefore,the proteins of the present invention are also predicted to beassociated with the above-mentioned diseases.

Due to a comparison of the nucleotide sequence shown in SEQ ID NO: 3with that shown in SEQ ID NO: 4, it can be seen that they share a commonsequence. In such a case, to confirm that these molecules were notartificially formed during the cDNA library production, the human genomelibrary is screened using sequences specific to the nucleotide sequenceshown in SEQ ID NO: 3 or 4, and the nucleotide sequence of the obtainedgenomic clones are determined.

Commercially available products (for example, BAC library from ResearchGenetics) can be used as the human genome library of the presentinvention; or it may be prepared from human cells or tissues using knownmethods (methods described in Genomics, 29, 413 (1995); Genomics, 24,527 (1994); etc.).

Examples of methods for screening human genome library using a sequencespecific to the nucleotide sequence shown in SEQ ID NO: 3 or 4 include:PCR methods using primers specific to the nucleotide sequence shown inSEQ ID NO: 3 or 4 (PCR Protocols, Academic Press (1990)); colonyhybridization or plaque hybridization methods (Molecular Cloning 2ndedition) using oligonucleotides specific to the nucleotide sequenceshown in SEQ ID NO: 3 or 4; and the like.

According to the above-mentioned method, a genomic DNA clone (forexample, human genome BAC clone) containing both of the nucleotidesequences shown in SEQ ID NO: 3 and SEQ ID NO: 4 can be obtained.Determination of the nucleotide sequence of this genomic DNA followed bythe comparison with the nucleotide sequences shown in SEQ ID NO: 3 andSEQ ID NO: 4 reveal that the nucleotide sequence shown in SEQ ID NO: 3and the nucleotide sequence shown in SEQ ID NO: 4 exist over a span ofapproximately 7 kb on the same human genome separated into 5 and 7regions, respectively. That is, the protein having the amino acidsequence shown in SEQ ID NO: 1 is composed of 5 exons and the proteinhaving the amino acid sequence shown in SEQ ID NO: 2 is composed of 7exons; and the second and fourth exons of the genes are completelyconcordant to each other. However, the beginning of the third exon andthe end of the fifth exon are different from each other, which result inthe difference observed by comparing the amino acid sequences shown inSEQ ID NOS: 1 and 2. Therefore, the protein having the amino acidsequence shown in SEQ ID NO: 1 and the protein having the amino acidsequence shown in SEQ ID NO: 2 are shown to be discrete products ofalternately spliced forms derived from the same gene, which show anunique characteristic of having different amino acid sequences from eachother.

Once the DNA consisting of the nucleotide sequence shown in SEQ ID NO: 3or 4 is obtained and the nucleotide sequence thereof is determined, aDNA of the present invention can be obtained by preparing primers basedon the 5′ end and 3′ end nucleotide sequences of such nucleotidesequence, and amplifying DNA by PCR method (PCR Protocols, AcademicPress (1990)) using cDNAs or a cDNA library synthesized from mRNAscontained in tissues or cells, such as small intestine of human ornon-human animal as a template.

Alternatively, a DNA of the present invention can be obtained using afull length DNA shown in SEQ ID NO: 3 or 4, or parts thereof as a probeto conduct colony hybridization or plaque hybridization (MolecularCloning, 2nd edition) against cDNAs or cDNA libraries synthesized frommRNAs contained in tissues or cells, such as small intestine of human ornon-human animal.

Furthermore, based on the determined nucleotide sequence of the DNA, aDNA of the present invention can be obtained by chemical synthesis usingDNA synthesizer, such as DNA synthesizer model 392 from Perkin Elmer,utilizing the phosphoramidite method.

The obtained DNA can be confirmed as a DNA encoding a protein having theactivity as the IGFBP superfamily by expressing the protein using atransformant that can be obtained by transforming a recombinant vectorcontaining the DNA into a host cell, or by comparing the identity andthe cysteine residue positions between the amino acid sequence encodedby such DNA and the amino acid sequence of human IGFBP-1, human IGFBP-2,human IGFBP-3, human IGFBP-4, human IGFBP-5, human IGFBP-6, humanIGFBP-7, human IGFBP-8, human IGFBP-9, or human IGFBP-10.

Based on the information relating to the nucleotide sequences shown inSEQ ID NOS: 3 and 4 or the nucleotide sequence of a fragment thereof,oligonucleotides that have the nucleotide sequence of the present DNA,for example, one corresponding to 5 to 60, preferably 10 to 40continuous bases of the nucleotide sequence shown in SEQ ID NO: 3 or 4,and oligonucleotides corresponding to sequences that are complementaryto the above oligonucleotides (hereinafter, referred to as antisenseoligonucleotide) can be prepared using ordinary methods or DNAsynthesizer.

Examples of oligonucleotides of the present invention includeoligonucleotides, such as oligo-DNA and oligo-RNA, derivatives of sucholigonucleotides (hereinafter, referred to as oligonucleotidederivatives), and so on.

Such oligonucleotides or antisense oligonucleotides are exemplified bysense primers corresponding to the 5′ end nucleotide sequence of a partof the nucleotide sequence of a mRNA to be detected, antisense primerscorresponding to the 3′ end nucleotide sequence of the mRNA, and so on.However, the base corresponding to uracil of the mRNA is thymidine inthe oligonucleotide primer.

Examples of the sense primers and antisense primers includeoligonucleotides, whose melting temperature (Tm) and number of bases donot change excessively, and the number of bases is 5 to 60, preferably10 to 50.

The oligonucleotide derivatives are exemplified by oligonucleotidederivatives wherein the phosphodiester bond of the oligonucleotide hasbeen converted to a phosphorothioate bond; oligonucleotide derivativeswherein phosphodiester bond in the oligonucleotide has been converted toa N3′-P5′ phosphoramidate bond; oligonucleotide derivatives wherein thephosphodiester bond between ribose and phosphate in the oligonucleotidehas been converted to a peptide-nucleic acid bond; oligonucleotidederivatives wherein uracil of the oligonucleotide has been substitutedwith C-5 propynyluracil; oligonucleotide derivatives wherein uracil ofthe oligonucleotide has been substituted with C-5 thiazoleuracil;oligonucleotide derivatives wherein cytosine of the oligonucleotide hasbeen substituted with C-5 propynylcytosine; oligonucleotide derivativeswherein cytosine of the oligonucleotide has been substituted withphenoxazine-modified cytosine; oligonucleotide derivatives whereinribose of the oligonucleotide has been substituted with2′-O-propylribose; oligonucleotide derivatives wherein ribose of theoligonucleotide has been substituted with 2′-methoxyethoxyribose, andthe like (Cell Technology, 16, 1463 (1997)).

2. Production of protein or polypeptide of the present invention

A protein or polypeptide of the present invention may be produced byexpression of a DNA of the present invention in host cells using methodsdescribed in Molecular Cloning Second Edition, Current Protocols inMolecular Biology, and the like, for example, by the following manner.

Based on a full-length cDNA, if necessary, a DNA fragment of anappropriate length containing a region coding for the protein isprepared.

The DNA fragment or the full-length cDNA is inserted downstream of apromoter of an appropriate expression vector to prepare a recombinantvector.

By introducing the recombinant vector into host cells suitable for theexpression vector, a transformant which produces the protein of thepresent invention can be obtained.

With regard to a host cell, any cell may be used as long as it iscapable of expressing the desired gene. Such a host cell includesbacteria, yeast, animal cells, insect cells, plant cells, etc.

An expression vector that can be used is autonomously replicable in theabove host cell or is able to be integrated into chromosomes andcontains a promoter at the position where the DNA coding for the proteinof the present invention is transcribed.

When a prokaryote such as bacteria is used as a host cell, it ispreferred that the recombinant vector containing the DNA coding for theprotein of the present invention is autonomously replicable inprokaryote and that it is a vector containing a promoter, aribosome-binding sequence, the gene coding for the protein of thepresent invention, and a transcription termination sequence. The vectormay also contain a gene that regulates the promoter.

Examples of the expression vector are pBTrp2, pBTac1, and pBTac2 (allsold by Boehringer-Mannheim); pKK233-2 (Pharmacia); pSE280 (Invitrogen);pGEMEX-1 (Promega); pQE-8 (Qiagen); pKYP10 (Japanese PublishedUnexamined Patent Application No. 110600/83); pKYP200 [Agric. Biol.Chem., 48, 669 (1984)]; pLSA1 [Agric. Biol. Chem., 53, 277 (1989)];pGEL1 [Proc. Natl. Acad. Sci. USA, 82, 4306 (1985)]; pBluescript IISK(−)(Stratagene); pTrs30 [prepared from Escherichia coli JM109/pTrS30(FERM BP-5407)]; pTrs32 [prepared from Escherichia coli JM109/pTrS32(FERM BP-5408)]; pGHA2 [prepared from Escherichia coli IGHA2 (FERMB-400), Japanese Published Unexamined Patent Application No. 221091/85];pGKA2 [prepared from Escherichia coli IGKA2 (FERM BP-6798), JapanesePublished Unexamined Patent Application No. 221091/85]; pTerm2 (U.S.Pat. Nos. 4,686,191, 4,939,094, and 5,160,735); pSupex, pUB 110, pTP5,pC194, and pEG400 [J. Bacteriol., 172, 2392 (1990)]; pGEX (Pharmacia);pET system (Novagen); and pSupex.

With regard to a promoter, any promoter may be used as long as it isable to function in host cells, and its examples are promoters derivedfrom Escherichia coli, phage, and the like, including trp promoter(P_(trp)), lac promoter, P_(L) promoter, P_(R) promoter, and T7promoter. It is also possible to use artificially designed and modifiedpromoters, such as a promoter where two P_(trp) are connected in tandem(P_(trp)×2), tac promoter, lacT7 promoter, and letI promoter.

It is preferred to use a plasmid where the distance between aninitiation codon and Shine-Dalgarno sequence, which is aribosome-binding sequence, is adjusted appropriately (for example, 6 to18 nucleotides).

It is possible to improve the productivity of the desired protein bysubstituting nucleotide(s) in the nucleotide sequence in the regioncoding for the protein so as to give a codon which is optimum for theexpression of the protein in the host.

In the recombinant vector of the present invention, although atranscription termination sequence is not always necessary for theexpression of a DNA of the present invention, it is preferred to place atranscription termination sequence immediately downstream of thestructural gene.

Examples of the host cell are microorganisms belonging to the genusEscherichia, the genus Serratia, the genus Bacillus, the genusBrevibacterium, the genus Corynebacterium, the genus Microbacterium, andthe genus Pseudomonas, such as Escherichia coli XL1-Blue, Escherichiacoli XL2-Blue, Escherichia coli DH1, Escherichia coli MC1000,Escherichia coli KY3276, Escherichia coli W1485, Escherichia coli JM109,Escherichia coli HB101, Escherichia coli No. 49, Escherichia coli W3110,Escherichia coli NY49, Serratia ficaria, Serratia fonticola, Serratialiquefaciens, Serratia marcescens, Bacillus subtilis, Bacillusamyloliquefaciens, Brevibacterium immariophilum ATCC 14068,Brevibacterium saccharolyticum ATCC 14066, Brevibacterium flavum ATCC14067, Brevibacterium ammoniagenes, Brevibacterium lactofermentum ATCC13869, Corynebacterium glutamicum ATCC 13032, Corynebacteriumacetoacidophilum ATCC 13870, Microbacterium ammoniaphilum ATCC 15354,Pseudomonas sp. D-0110, and the like.

A recombinant vector can be introduced into the above-mentioned hostcells by any of methods for the introduction of DNA, for example, themethod using calcium ion [Proc. Natl. Acad. Sci. USA, 69, 2110 (1972)],protoplast method (Japanese Published Unexamined Patent Application No.248394/88), and the methods described in Gene, 17, 107 (1982) andMolecular & General Genetics, 168, 111 (1979).

When yeast is used as a host cell, examples for expression vector areYEP13 (ATCC 37115), YEp24 (ATCC 37051), and YCp50 (ATCC 37419).

With regard to a promoter, any promoter may be used as long as it isable to function in yeast cell lines, and its examples are promoters ofgenes of a glycolytic pathway such as hexokinase, PHO5 promoter, PGKpromoter, GAP promoter, ADH promoter, gal 1 promoter, gal 10 promoter,heat shock protein promoter, MFα1 promoter, and CUP 1 promoter.

Examples of the host cell are microorganisms belonging to the genusSaccharomyces, the genus Kluyveromyces, the genus Trichosporon, and thegenus Schwanniomyces, such as Saccharomyces cerevisiae,Schizosaccharomyces pombe, Kluyveromyces lactis, Trichosporon pullulans,and Schwanniomyces alluvius.

A recombinant vector can be introduced into yeast by any method as longas it enables introduction of DNA into yeast. Such a method includes theelectroporation method [Methods. Enzymol., 194, 182 (1990)], thespheroplast method [Proc. Natl. Acad. Sci. USA, 84, 1929 (1978)], thelithium acetate method [J. Bacteriology, 153, 163 (1983)], and themethod described in Proc. Natl. Acad. Sci. USA, 75, 1929 (1978).

When an animal cell is used as a host, examples of expression vectorsinclude pcDNAI, pcDM8 (Funakoshi), pAGE107 [Japanese PublishedUnexamined Patent Application No. 22979/91; Cytotechnology, 3, 133(1990)], pAS3-3 (Japanese Published Unexamined Patent Application No.227075/90), pCDM8 [Nature, 329, 840 (1987)], pcDNAI/Amp (Invitrogen),pREP4 (Invitrogen), pAGE103 [J. Biochemistry, 101, 1307 (1987)], andpAGE210.

With regard to a promoter, any promoter may be used as long as it isable to function in animal cells, such as the promoter of immediateearly (IE) gene of cytomegalovirus (CMV), early promoter of SV40,promoter of retrovirus, metallothionein promoter, heat shock promoter,and SRα promoter. It is also possible to use an enhancer for IE gene ofhuman CMV together with a promoter.

Examples of the host cell are Namalwa cell, which is a human cell; COScell, which is a simian cell; CHO cell, which is a cell of Chinesehamster; and HBT5637 (Japanese Published Unexamined Patent ApplicationNo. 299/88).

A recombinant vector can be introduced into animal cells by any methodas long as the method enables introduction of DNA into animal cells, andits examples are the electroporation method [Cytotechnology, 3, 133(1990)], the calcium phosphate method (Japanese Published UnexaminedPatent Application No. 227075/90), and the lipofection method [Proc.Natl. Acad. Sci. USA, 84, 7413 (1987)].

When an insect cell is used as a host, it is possible to express aprotein by the method described in, for example, “Current Protocols inMolecular Biology”; “Baculovirus Expression Vectors, A LaboratoryManual”, W.H. Freeman and Company, New York (1992); and Bio/Technology,6, 47 (1988).

Thus, a recombinant gene-introducing vector and a baculovirus areco-transfected into an insect cell to give a recombinant virus in asupernatant of insect cell culture, and then a recombinant virus isfurther infected to another insect cell to express a protein.

Examples of the gene-introducing vector used in such a method are pVL1392, pVL 1393, and pBlueBacIII (all from Invitrogen).

Baculoviruses that can be used include, for example, Autographacalifornica nuclear polyhedrosis virus, which is a virus infecting toinsects belonging to the subfamily Hadeninae.

Examples of an insect cell include Sf9 and Sf21, which are ovary cellsof Spodoptera frugiperda [“Baculovirus Expression Vectors, A LaboratoryManual”, W.H. Freeman and Company, New York (1992)]; and High 5(Invitrogen), which is an ovary cell of Trichoplusia ni.

The above-mentioned recombinant gene-introducing vector and theabove-mentioned baculovirus can be co-transfected into an insect cellfor the preparation of a recombinant virus by, for example, the calciumphosphate method (Japanese Published Unexamined Patent Application No.227075/90), the lipofection method [Proc. Natl. Acad. Sci. USA, 84, 7413(1987)], and the like.

When a plant cell is used as a host cell, examples of an expressionvector are Ti plasmid and tobacco mosaic virus vector.

Any promoter may be used as long as it is able to function in a plantcell, and its examples are 35S promoter of cauliflower mosaic virus(CaMV) and rice actin 1 promoter.

Examples of the host cell are cells of plants such as tobacco, potato,tomato, carrot, soybean, rape, alfalfa, rice, wheat, and barley.

A recombinant vector can be introduced by any method as long as themethod enables introduction of DNA into a plant cell, and its examplesare methods using Agrobacterium (Japanese Published Unexamined PatentApplication No. 140885/84 and 70080/85 and WO94/00977), theelectroporation method (Japanese Published Unexamined Patent ApplicationNo. 251887/85), and methods using particle gun (Japanese Patent NOS.2606856 and 2517813).

When yeast, animal cells, insect cells, or plant cells are used as hostcells, it is possible to obtain the protein or the polypeptide to whichsugar or sugar chain is added.

The transformant prepared as above is cultured in a medium, and theprotein or the polypeptide of the present invention is produced andaccumulated therein and then recovered from the culture, to produce theprotein or the polypeptide of the present invention. The culturing thetransformant of the present invention in a medium can be carried outaccording to common methods used for culturing the host.

A medium for culturing a transformant obtained using prokaryotes, suchas Escherichia coli, or eukaryotes, such as yeast, as a host, may be anyof natural and synthetic media as long as the medium contains carbonsource, nitrogen source, inorganic salts, and the like, which can beassimilated by the organism and enables culturing the transformantefficiently.

Any source may be used as a carbon source as long as the organism isable to assimilate it, and its examples are carbohydrates, such asglucose, fructose, sucrose, molasses containing them, starch andhydrolyzed starch; organic acids, such as acetic acid and propionicacid; and alcohols, such as ethanol and propanol.

Examples of a nitrogen source include ammonia; ammonium salts ofinorganic or organic acid, such as ammonium chloride, ammonium sulfate,ammonium acetate, and ammonium phosphate; other nitrogen-containingcompounds; peptone; meat extract; yeast extract; corn steep liquor;hydrolyzed casein; soybean cake; hydrolyzed soybean cake; variousfermented cells and digested products thereof; and the like.

Examples of an inorganic substance include potassium dihydrogenphosphate, dipotassium hydrogen phosphate, magnesium phosphate,magnesium sulfate, sodium chloride, ferrous sulfate, manganese sulfate,copper sulfate, calcium carbonate, and the like.

Usually, the transformant is cultured under an aerobic condition, forexample, by shaking culture or submerged-aerated spinner culture.Temperature for the culturing is preferably 15 to 40° C., and time forthe culturing is usually from 16 hours to seven days. The pH during theculturing is kept at pH 3.0 to pH 9.0. The pH is adjusted with inorganicor organic acid, an alkaline solution, urea, calcium carbonate, ammonia,etc.

During the culturing, antibiotic, such as ampicillin or tetracycline,may be added to the medium if necessary.

In the case of culturing a microorganism transformed with a recombinantvector in which an inducible promoter is used as a promoter, an inducermay be added to the medium if necessary. For example, when amicroorganism transformed with a recombinant vector containing lacpromoter is cultured, isopropyl-β-D-thiogalactopyranoside and the likemay be added to the medium. When a microorganism transformed with arecombinant vector containing trp promoter is cultured, indole acrylicacid and the like may be added to the medium.

As the medium for a transformant obtained using animal cell as a host,the commonly used RPMI1640 medium [The Journal of the American MedicalAssociation, 199, 519 (1967)], Eagle's MEM medium [Science, 122, 501(1952)], modified Dulbecco's MEM medium [Virology, 8, 396 (1959)], 199medium [Proceeding of the Society for the Biological Medicine, 73, 1(1950)], a medium prepared by adding fetal calf serum, etc. to any ofthese media, and the like can be used.

The culturing is usually carried out for 1 to 7 days under theconditions, for example, of pH 6 to 8 at 30 to 40° C. in the presence of5% CO₂.

During the culturing, antibiotics, such as kanamycin or penicillin, maybe added to the medium if necessary.

As a medium for a transformant obtained using insect cell as a host, thecommonly used TNM-FH medium (Pharmingen); Sf-900 II SFM medium (LifeTechnologies); ExCell400 and ExCell405 (both JRH Biosciences); Grace'sInsect Medium (Grace, T. C. C., Nature, 195, 788 (1962)); and the likecan be used.

The culturing is usually carried out for 1 to 5 days under theconditions, for example, of pH 6 to 7 at 25 to 30° C.

During the culturing, antibiotics, such as gentamycin, may be added tothe medium if necessary.

A transformant obtained using plant cell as a host can be cultured inthe form of cell or differentiated cell or organ of the plant. As themedium for the transformant, the commonly used Murashige and Skoog (MS)medium, White medium, or a medium prepared by adding phytohormone, suchas auxin or cytokinin, to any of these medium, and the like can be used.

The culturing is usually carried out for 3 to 60 days under theconditions of pH 5 to 9 at 20 to 40° C.

During the culturing, antibiotics, such as kanamycin or hygromycin, maybe added to a medium if necessary.

As mentioned above, a transformant derived from a microorganism, animalcell, or plant cell having a recombinant vector into which the DNAcoding for the protein of the present invention has been incorporated iscultured by a conventional culturing method, and the protein or thepolypeptide is produced and accumulated therein and is recoveredtherefrom to prepare the protein or the polypeptide.

The method for producing the protein or the polypeptide of the presentinvention includes intracellular production by host cells, extracellularsecretion by host cells, production as a fusion protein, or productionon outer membranes by host cells, and the method can be selecteddepending on the host cells used or on alteration of the structure ofthe protein to be produced.

When the protein of the present invention is produced inside of the hostcell or on an outer membrane of the host cell, it is possible to secretethe protein extracellular portion of the host cell according to themethod of Paulson et al. [J. Biol. Chem., 264, 17619 (1989)]; the methodof Row et al. [Proc. Natl. Acad. Sci., USA, 86, 8227 (1989); GenesDevelop., 4, 1288 (1990)], or the methods described in JapanesePublished Unexamined Patent Application No. 336963/2003 and WO94/23021,etc.

Specifically, it is possible to secrete the protein of the presentinvention extracellular portion of the host cell vigorously byexpressing the protein to which a signal peptide has been added to theupstream of the protein containing the active site of the protein bymeans of genetic engineering technique.

It is also possible to increase the production amount of the proteinutilizing a gene-amplification system using a dihydrofolate reductasegene or such according to the method described in Japanese PublishedUnexamined Patent Application No. 227075/91.

Furthermore, by redifferentiating gene-transferred cells of animals orplants, individual gene-transferred animals (transgenic non-humananimals) or plants (transgenic plants) are prepared, and using theseindividuals, a protein of the present invention can be produced.

When the transformant is an individual animal or plant, the protein canbe produced by raising or cultivating the individual, generating andaccumulating the protein, and then recovering the protein from theindividual animal or plant according to conventional methods.

For example, a method for producing a protein of the present inventionusing an individual animal includes a method for producing the proteinof the present invention in an animal produced by transferring a genefollowing known procedures (American Journal of Clinical Nutrition, 63,639S (1996); American Journal of Clinical Nutrition, 63, 627S (1996);Bio/Technology, 9, 830 (1991)).

In the case of individual animals, the protein can be produced, forexample, by raising a transgenic non-human animal transfected with DNAencoding the protein of the present invention; producing andaccumulating the protein in the animal; and recovering the protein fromthe animal. For example, the place of production and accumulation in theanimal may be milk (Japanese Published Unexamined Patent Application No.309192/88), egg, and the like of the animal. Any promoter can be used solong as it can be expressed in the animal, and preferably includes, forexample, mammary cell specific promoters such as α-casein promoter,β-casein promoter, β-lactoglobulin promoter, and whey acidic proteinpromoter.

Methods for producing a protein of the present invention usingindividual plants include, for example, a method wherein the protein isproduced by cultivating a transgenic plant transfected with a DNAencoding the protein of the present invention according to knownprocedures (Tissue Culture, 20 (1994); Tissue Culture 21 (1995); Trendsin Biotechnology, 15, 45 (1997)), producing and accumulating the proteinwithin the plant, and then recovering the protein from the plant.

For example, when a protein of the present invention is expressed in asolubilized form within a cell, after cultivation, the cells arerecovered by centrifugation; suspended into an aqueous buffer;homogenized by an ultrasonic homogenizer, French press, Manton Gaulinhomogenizer, Dynomill, and the like; and the protein produced by thetransformant of the present invention is obtained as a cell free extractsolution. A purified preparation can be obtained from the supernatantobtained by centrifugation of the cell-free extract solution usingeither any one of conventional enzyme isolation and purification methodsor in combination, such as the solvent extraction methods; the altprecipitation methods by ammonium sulfate and the like; desaltingmethods; precipitation by organic solvents; anion exchangechromatography methods using resins, such as diethylaminoethyl(DEAE)-sepharose, and DIAION HPA-75 (Mitsubishi Chemicals); cationexchange chromatography methods using resins, such as S-Sepharose FF(Pharmacia); hydrophobic chromatography methods using resins, such asbutyl sepharose and phenyl sepharose; gel filtration methods usingmolecular sieves; affinity chromatography methods; chromatofocusingmethods; and electrophoretic methods, such as isoelectricelectrophoresis.

Furthermore, when a protein is expressed as insoluble bodies within acell, similarly, the cells are recovered, homogenized, and thencentrifuged to obtain the insoluble bodies of the protein as aprecipitated fraction. The recovered insoluble bodies of the protein aresolubilized with a protein denaturant. After restoring the normalthree-dimensional structure of the protein by diluting or dialyzing thesolubilized solution, a purified preparation of the protein can beobtained by isolation and purification methods similar to thosementioned above.

When a protein of the present invention or the derivative thereof, suchas glycosylation products, is secreted outside of the cell, the proteinor the derivative thereof, such as sugar chain adducts, can be recoveredas the culture supernatant. Specifically, a soluble fraction is obtainedby treating the culture with techniques, such as centrifugation, similarto those mentioned above, and then the purified preparation can beobtained from the soluble fraction using isolation and purificationmethods similar to those mentioned above.

An example of a protein obtained in this manner is, for example, aprotein having the amino acid sequence shown in SEQ ID NO: 1 or SEQ IDNO: 2.

Alternatively, a protein of the present invention can be produced bychemical synthesis methods, such as Fmoc method(fluorenylmethoxycarbonyl method) and tBoc method (t-butoxycarbonylmethod). Furthermore, chemical synthesis can be performed utilizingpeptide synthesizers from Advanced ChemTech, Perkin Elmer, Pharmacia,Protein Technology Instrument, Synthecell-Vega, PerSeptive, Shimadzu,and the like.

3. Preparation of Antibodies Recognizing the Protein of the PresentInvention

Using the proteins of the present invention, purified preparations ofpartial fragment polypeptides of these proteins, or peptides containinga partial amino acid sequence of the protein of the present invention asantigens, antibodies, such as polyclonal antibodies and monoclonalantibodies, recognizing the proteins of the present invention can beproduced.

(1) Preparation of Polyclonal Antibody

The proteins of the present invention, purified samples of a partialpolypeptide fragment of the proteins, and peptides comprising a partialamino acid sequences of the proteins of the present invention can beused as an antigen and administered into an animal to prepare apolyclonal antibody.

Such animals that can be utilized for the administration include rabbit,goat, rat, mouse, hamster, etc.

It is preferred to use the antigen in an administration dose of 50 μg to100 μg per animal.

When a peptide is used for this purpose, it is preferred to use thepeptide as an antigen after covalently linked to a carrier protein, suchas keyhole limpet haemocyanin (KLH) or bovine thyroglobulin. The peptideto be used as the antigen can be synthesized by a peptide synthesizer.

After the first administration, the antigen is given 3 to 10 times at 1to 2-week intervals. 3 to 7 days after each time of administration,blood is sampled from the venous plexus of eyegrounds. Then the serum istested for the reactivity to the antigen used for the immunization by amethod of enzyme immuno-assay (“Methods of Enzyme Immuno-Assay (ELISA):Igakushoin, 1976; “Antibodies: A Laboratory Manual”, Cold Spring HarborLaboratory Press (1988)), etc.

It is possible to obtain the polyclonal antibody by collecting the serafrom non-human mammals that have exhibited sufficiently high antibodytiters in their sera against the antigen used for the immunization, andseparating and purifying the sera.

Such methods for the separation and purification include centrifugalseparation, salting out with 40% to 50% saturated ammonium sulfate,precipitation by caprylic acid (“Antibodies: A Laboratory Manual”, ColdSpring Harbor Laboratory (1988)), and a procedure using chromatographicmethods, e.g., using DEAE-Sepharose column, anion exchange column,protein-A or -G column, gel filtration column, etc. singly or incombination.

(2) Preparation of Monoclonal Antibody

(a) Preparation of Antibody-Producing Cells

Rats, whose sera have exhibited sufficiently high titers of antibodyagainst a partial polypeptide fragment of the present invention used forthe immunization, are provided as the source of antibody-producingcells.

3 to 7 days after the final administration of the antigen substance tothe rats that have exhibited such light titers of antibody, theirspleens are removed from them.

The spleens are sectioned into small pieces in MEM medium (NissuiPharmaceutical Co., Ltd.) and loosened by forceps. After centrifugationat 1,200 rpm for 5 minutes, the supernatant is discarded.

The resulting precipitated fraction of spleen cells is treated withTris-ammonium chloride buffer (pH 7.65) for 1 to 2 minutes to remove redblood cells, and then the spleen cells are washed 3 times with MEM. Thespleen cells prepared are used as antibody-producing cells.

(b) Preparation of Myeloma Cells

Myeloma cell to be used is a cell line established from mouse or rat.For example, 8-azaguanine resistant mouse (BALB/c-derived) myeloma celllines that are usable include P3-X63Ag8-U1 (hereinafter, abbreviated as“P3-U1”) (Curr. Topics Microbiol. Immunol., 81, 1 (1978); Eur. J.Immunol., 6, 511 (1976)), SP2/0-Ag14(SP-2) (Nature, 276, 269 (1978)),P3-X63-8653(653) (J. Immunol., 123, 1548 (1979)), P3-X63-Ag(×63)(Nature, 256, 495 (1975)), and the like. Cells of these lines arepassaged in 8-azaguanine medium [RPMI1640 medium containing glutamine(1.5 mmol/L), 2-mercaptomethanol (5×10−5 mol/L), gentamicin (10 μg/ml),and fetal calf serum (FCS) (CSL, 10%) (hereinafter referred to as normalmedium) further containing 8-azaguanine (15 μg/ml)], but 3 to 4 daysbefore the cell fusion the cells are cultured in the normal medium.2×10⁷ or more cells are used for the fusion.

(c) Preparation of Hybridoma

The antibody-producing cells prepared as described in (a) and myelomacells in (b) are washed well with MEM or PBS (1.83 g of disodiumphosphate, 0.21 g of potassium dihydrogenphosphate, 7.65 g of sodiumchloride, 1 L of distilled water; pH 7.2); mixed with each other at aratio of the numbers of antibody-producing cells: myeloma cells=5 to10:1. After the mixture was subjected to centrifugation at 1,200 rpm for5 minutes, the supernatant is discarded.

The mixed cells prepared from the precipitated fraction are welldispersed. While the cells are being stirred at 37° C., 0.2 to 1 ml (per108 antibody-producing cells) of a solution of 2 gPolyethyleneglycol-1000 (PEG-1000), 2 ml MEM, and 0.7 mldimethylsulfoxide (DMSO) is added to the cell mixture; then 1 to 2 ml ofMEM is added thereto several times at 1 to 2-minute intervals. After theaddition, the cells are so prepared by further adding MEM medium thatthe total volume becomes 50 ml. The prepared mixture is subjected tocentrifugation at 900 rpm for 5 minutes, and then the supernatant isdiscarded. The cells from the resulting precipitated fraction are gentlydispersed, suspended by gentle pipetting with a measuring pipette in 100ml of HAT medium (a medium for which hypoxanthine (10⁻⁴ mol/L),thymidine (1.5×10⁻⁵ mol/L), and aminopterin (4×10⁻⁷ mol/L) have beenadded to the normal medium).

A 100-μl aliquot of the suspension was dispensed into each well of a96-well culture plate. Then the cells are cultured in an incubator with5% CO₂ at 37° C. for 7 to 14 days.

After the culture is completed, an aliquot of the culture supernatant isutilized for the selection of hybridomas specifically reacting with apartial polypeptide fragment of the protein of the present inventionaccording to enzyme immuno-assay methods described in “Antibodies, ALaboratory Manual” (Cold Spring Harbor Laboratory, Chapter 14 (1988)),and the like.

A specific example of the enzyme immuno-assay method is as follows.

An appropriate plate is coated with a partial peptide fragment of aprotein of the present invention used as an antigen for theimmunization. The hybridoma culture supernatant or purified antibodyobtained in (d) as described below is reacted as a primary antibody, andan anti-rat immunoglobulin or an anti-mouse immunoglobulin antibodylabeled with biotin, enzyme, chemically-luminescent substance,radioisotope, or the like is further reacted as a secondary antibody inthe plate. Subsequently a reaction is carried out according to the labelsubstance and cells exhibiting the specific reactivity to the protein ofthe present invention are selected as hybridomas producing monoclonalantibody against the protein of the present invention.

The hybridomas are cloned twice by the limiting dilution method [with HTmedium (HAT medium without aminopterin) in the first cloning, and withthe normal medium in the second]. Cells that stably exhibit highantibody titers are selected as hybridoma lines producing monoclonalantibody of the present invention.

(d) Preparation of Monoclonal Antibody

The hybridoma cells obtained in (c) producing the protein monoclonalantibody of the present invention are intraperitoneally injected (5 to20×10⁶ cells per mouse) to 8 to 10-week mice or nude mice treated withpristane (which have been subjected to intraperitoneal administration of0.5 ml of 2,6,10,14-tetramethylpentadecane (pristane) and have been bredfor 2 weeks). The hybridomas form ascites carcinoma in 10 to 21 days.

The ascites is collected from each mouse having ascites tumor, and thenis subjected to centrifugation at 3000 rpm for 5 minutes to remove thesolid material.

The monoclonal antibodies can be purified and prepared from theresulting supernatant by the same method as used for the preparation ofpolyclonal antibody.

Subtyping of antibody can be performed using a typing kit for mouse orrat monoclonal antibody. The quantity of protein can be calculatedaccording to Lowry method or by using absorbance at 280 nm.

4. Use of DNAs, Proteins, or Antibodies of the Present Invention

(1) Method for Detecting Diseases by Detecting and Quantifying theExpression of DNA Encoding a Protein of the Present Invention

Diseases, which are listed below, that are reported to be related toproteins belonging to the IGFBP superfamily can be detected byperforming Northern hybridization method (Molecular Cloning 2ndedition); PCR method, and RT (reverse-transcribed)-PCR method (both inPCR Protocols, Academic Press (1990)) (all together referred to as PCRmethod); and the like using the DNAs or oligonucleotides of the presentinvention to detect the expression of DNAs encoding a protein of thepresent invention: diseases accompanying abnormal cell growth, such asacute myelogenous leukemia, breast cancer, prostate cancer, coloncancer, hepatic cancer, myeloma, uterine leiomyoma, malignant tumor, andsolid tumor; diseases accompanying angiopathy, such as myocardialinfarction, cerebral infarction, peripheral vascular atresia, anginapectoris, hypertension, hyperlipidemia, diabetes, diabetic retinopathy,glomerulonephritis, arteriosclerosis, thrombosis, hemolytic uremicsyndrome, thrombotic thrombocytopenic purpa, ischemic heart disease,ischemic encephalopathy, heart failure, hemostasis, and choroidcirculatory dysfunction; diseases accompanying abnormal bone metabolismsuch as osteoporosis; diseases accompanying disorders of insulin-likegrowth factors or growth hormone action, such as dwarfism, acromegaly,and infantile chronic renal failure; diseases accompanying abnormaldifferentiation or growth of smooth muscle cells, such asarteriosclerosis, bronchial disease, and restenosis; diseasesaccompanying abnormal differentiation or growth of skeletal musclecells, such as myasthenia gravis; diseases accompanying abnormal gastricacid secretion such as gastric ulcer; and inflammatory diseasesaccompanying abnormal lymphocyte invasion, such as microbial infection,chronic hepatitis B, chronic rheumatoid arthritis, sepsis, graft versushost disease, insulin dependent diabetes mellitus, nephritis, traumaticbrain damage, inflammatory enteropathy, allergy, atopy, asthma,pollinosis, airway hypersensitivity; etc.

Since RT-PCR method is a simple and convenient method, it is especiallyuseful as a method for detecting the expression of DNAs.

For example, the above-mentioned diseases can be detected by performingNorthern hybridization using a DNA having the same sequence as 100 to1772 continuous bases in the nucleotide sequence shown in SEQ ID NO: 3,a DNA having a complementary sequence to such DNA, a DNA having the samesequence as 100 to 2698 continuous bases in the nucleotide sequenceshown in SEQ ID NO: 4, or a DNA having a sequence complementary to suchDNA as probes; quantifying the expression level of the DNA of SEQ ID NO:3 or 4; and comparing the level to that of a healthy subject.

The following methods can be exemplified as specific methods for thedetection.

Total RNAs (10 to 20 μg) or mRNAs thereof (1 to 5 μg) derived fromleukocytes or tissues of a test subject and a healthy subject aredenatured in denaturing solution (50% (v/v) formamide, 2.2 mol/Lformaldehyde, 20 mmol/L MOPS (3-(N-morpholino)propanesulfonic acid) (pH7.0), 5 mmol/L sodium acetate, 1 mmol/L EDTA) by heating at 65° C. for 5minutes; and electrophoresis is performed using 1% agarose gelcontaining 2.2 mol/L formaldehyde.

After the electrophoresis, the RNAs in the gel are blotted onto anitrocellulose filter (Optimal BA-S85; Schleicher & Schuell) and isimmobilized by heating at 80° C. for 1 hour under reduced pressure.

Pre-hybridization is performed by soaking this filter in hybridizationsolution (5×SSPE (750 mmol/L NaCl, 50 mmol/L NaH₂PO₄, 5 mmol/L EDTA; pH7.4), 5×Denhardt's solution (0.1% ficoll, 0.1% polyvinyl pyrrolidone,0.1% bovine serum albumin), 1% SDS (sodium dodecyl sulfate), 0.2 mg/mlSalmon Sperm DNA (Pharmacia Biotech).

After the prehybridization, probes are added to the solution, andhybridization is performed at 65° C.

As a probe, for example, the DNA fragment of SEQ ID NO: 3 or the DNAfragment of SEQ ID NO: 4 that are labeled with ³²P using Multiprime DNALabeling System (Amersham) can be used.

After hybridization, the filter is washed in the following order:

-   -   (a) washing in 2×SSC solution (300 mmol/L NaCl, 30 mmol/L sodium        citrate) containing 0.1% SDS at room temperature for 15 minutes,        which is repeated several times;    -   (b) washing in 1×SSC solution (150 mmol/L NaCl, 15 mmol/L sodium        citrate) containing 0.1% SDS at 50 to 70° C. for 15 minutes,        which is repeated several times; and    -   (c) washing in 0.1×SSC solution (15 mmol/L NaCl, 1.5 mmol/L        sodium citrate) containing 0.1% SDS at 50 to 70° C. for 15        minutes, which is repeated several times.

After washing the filter, expression of the DNA of SEQ ID NO: 3 or 4 canbe detected and quantified with Bioimaging Analyzer BAS2000 (Fuji PhotoFilm) by performing autoradiography using an imaging plate.

Furthermore, the above-mentioned diseases can be detected, for exampleby performing PCR using a pair of oligonucleotides specific to a DNAencoding a protein of the present invention as a primer and cDNAsprepared from total RNAs derived from leukocytes or tissues of a testsubject and a healthy subject, mRNAs thereof, or cDNAs prepared fromthese RNAs as templates; detecting and quantifying the amplifiedfragments; and comparing the expression levels of the DNAs of the testsubject and the healthy subject.

The oligonucleotides of the present invention can be used as sucholigonucleotides.

Total RNA or mRNA, which serves as a template for PCR, can be extractedfrom various leukocytes separated and obtained from blood or fromtissues, such as tissues suspected of a disease.

Examples of leukocytes include polymorphonuclear leukocytes, monocytes,lymphocytes, T cells, B cells, and so on.

Polymorphonuclear leukocytes and mononuclear leukocytes can be separatedand obtained from peripheral blood of a subject using Polymorphprep™, akit manufactured by Nycomed Pharma.

From the obtained mononuclear leukocytes, monocytes and lymphocytes canbe separated and obtained by methods according to J. Immunol., 130, 706(1983), and the like. T cells and B cells can be separated and obtainedby methods according to Tissue Antigen, 9, 153 (1977); J. Immunol., 11,273 (1976); the instructions by Nycomed relating to isolation methods ofblood cells, and the like.

T cells can be obtained using the nylon wool method (Eur. J. Immunol.,3, 645 (1973)). Alternatively, each of the cells can be separated andobtained using magnetic beads (for example Dynabeads from Dynal) boundto antibodies specific to T cells, B cells, and monocytes/macrophage,respectively.

Examples of methods for preparing total RNA from leukocytes or tissuesinclude the guanidine thiocyanate-cesium trifluoroacetate method(Methods in Enzymol., 154, 3 (1987)), and the like.

Examples of methods for preparing poly(A)+ RNA from total RNA includethe oligo(dT)-immobilized cellulose column method (Molecular Cloning,2nd edition), and the like.

Furthermore, mRNA can be prepared using kits, such as Fast Track mRNAIsolation Kit (Invitrogen), and Quick Prep mRNA Purification Kit(Pharmacia).

Single-stranded cDNA can be synthesized from total RNA or mRNA usingsingle-stranded cDNA synthesis kit, Superscript preamplification system(BRL). Synthesis can be performed according to the instructions attachedto the kit.

The expression level of a gene encoding the protein of the presentinvention can be quantified by RT-PCR method (PCR Protocols, AcademicPress (1990)) using total RNA, mRNA, or cDNA that can be prepared asmentioned above.

(2) Method for Detecting Diseases by Detecting Mutations of a GeneEncoding a Protein of the Present Invention

Using the oligonucleotides of the present invention as a probe,mutations of a gene encoding a protein of the present invention can bedetected by performing Southern hybridization method (Molecular Cloning,2nd edition), PCR method, and the like on genomic DNA. The detectionmethod can be used for detecting diseases that have been reported to beassociated with a protein belonging to the IGFBP family including, forexample, diseases accompanying abnormal cell growth, such as acutemyelogenous leukemia, breast cancer, prostate cancer, colon cancer,hepatic cancer, myeloma, uterine leiomyoma, malignant tumor, and solidtumor; diseases accompanying angiopathy, such as myocardial infarction,cerebral infarction, peripheral vascular atresia, angina pectoris,hypertension, hyperlipidemia, diabetes, diabetic retinopathy,glomerulonephritis, arteriosclerosis, thrombosis, hemolytic uremicsyndrome, thrombotic thrombocytopenic purpa, ischemic heart disease,ischemic encephalopathy, heart failure, hemostasis, and choroidcirculatory dysfunction; diseases accompanying abnormal bone metabolism,such as osteoporosis; diseases accompanying disorders of insulin-likegrowth factors or growth hormone action, such as dwarfism, acromegaly,and infantile chronic renal failure; diseases accompanying abnormaldifferentiation or growth of smooth muscle cells, such asarteriosclerosis, bronchial disease, and restenosis; diseasesaccompanying abnormal differentiation or growth of skeletal musclecells, such as myasthenia gravis; diseases accompanying abnormal gastricacid secretion, such as gastric ulcer; and inflammatory diseasesaccompanying abnormal lymphocyte invasion, such as microbial infection,chronic hepatitis B, chronic rheumatoid arthritis, sepsis, graft versushost disease, insulin dependent diabetes mellitus, nephritis, traumaticbrain damage, inflammatory enteropathy, allergy, atopy, asthma,pollinosis, airway hypersensitivity, and autoimmune disease.

Specifically, the presence or absence of mutations in the coding regioncan be determined, for example, by amplifying the coding region of a DNAencoding a protein of the present invention carried by a patient by PCRmethod, determining the nucleotide sequence, and comparing thenucleotide sequence to the DNA carried by a healthy subject.

The cDNA that serves as the template for the PCR method can be obtainedby the method of (1). The nucleotide sequence of the obtained cDNA canbe determined using DNA Sequencer 377 from Perkin Elmer and reaction kit(ABI Prism™ BigDye™ Terminator Cycle Sequencing Ready Reaction kit:Applied Biosystems).

(3) Method for Detecting Diseases by Immunological Detection Method andQuantitative Determination Method

Using an antibody of the present invention, diseases mentioned below canbe detected by immunologically detecting or quantitatively determiningthe expression of a protein of the present invention in blood or tissuesof a test subject and a healthy subject, and then comparing the resultsbetween the test subject and the healthy subject.

Examples of methods for immunological detection are ELISA method usingmicrotiter plates, fluorescent antibody method, Western Blotting method,immunohistological staining method, and the like.

Examples of methods for immunological quantitative determination are thesandwich ELISA method using two types of monoclonal antibodies havingdifferent epitopes from among antibodies reacting with a protein of thepresent invention in a liquid phase; the radioimmunoassay method using aprotein of the present invention labeled with a radioisotope, such as¹²⁵I, and antibodies recognizing this protein; and the like.

The immunological methods can be used for detecting diseases reported tobe associated with a protein belonging to the IGFBP family including,for example, diseases accompanying abnormal cell growth, such as acutemyelogenous leukemia, breast cancer, prostate cancer, colon cancer,hepatic cancer, myeloma, uterine leiomyoma, malignant tumor, and solidtumor; diseases accompanying angiopathy, such as myocardial infarction,cerebral infarction, peripheral vascular atresia, angina pectoris,hypertension, hyperlipidemia, diabetes, diabetic retinopathy,glomerulonephritis, arteriosclerosis, thrombosis, hemolytic uremicsyndrome, thrombotic thrombocytopenic purpa, ischemic heart disease,ischemic encephalopathy, heart failure, hemostasis, and choroidcirculatory dysfunction; diseases accompanying abnormal bone metabolism,such as osteoporosis; diseases accompanying disorders of insulin-likegrowth factors or growth hormone action, such as dwarfism, acromegaly,and infantile chronic renal failure; diseases accompanying abnormaldifferentiation or growth of smooth muscle cells, such asarteriosclerosis, bronchial disease, and restenosis; diseasesaccompanying abnormal differentiation or growth of skeletal musclecells, such as myasthenia gravis; diseases accompanying abnormal gastricacid secretion, such as gastric ulcer; and inflammatory diseasesaccompanying abnormal lymphocyte invasion, such as microbial infection,chronic hepatitis B, chronic rheumatoid arthritis, sepsis, graft versushost disease, insulin dependent diabetes mellitus, nephritis, traumaticbrain damage, inflammatory enteropathy, allergy, atopy, asthma,pollinosis, airway hypersensitivity, and autoimmune disease.

Furthermore, using antibodies that specifically bind to the proteinconsisting of the amino acid sequence shown in SEQ ID NO: 1 andantibodies that specifically bind to the protein consisting of the aminoacid sequence shown in SEQ ID NO: 2, diseases characterized bydifferences in the expression ratios of these proteins can be determinedfrom the above-mentioned diseases by the above-mentioned immunologicalmethods.

(4) Method for Detecting Diseases by Analyzing the Expression Pattern ofa DNA or a Protein of the Present Invention

By quantitatively determining and comparing the expression levels of aDNA encoding a protein comprising the amino acid sequence shown in SEQID NO: 1 and a DNA encoding a protein comprising the amino acid sequenceshown in SEQ ID NO: 2 in blood or tissue of a test subject and a healthysubject using any one of the method of the above-mentioned (1) to (3)for quantitatively measuring the expression levels of the DNA or theprotein of the present invention, diseases characterized by a differentexpression level ratio of the DNAs or the proteins between the testsubject and the healthy subject can be determined.

The detection methods as described above can be used for detectingdiseases reported to be associated with proteins belonging to the IGFBPfamily including, for example, diseases accompanying abnormal cellgrowth, such as acute myelogenous leukemia, breast cancer, prostatecancer, colon cancer, hepatic cancer, myeloma, uterine leiomyoma,malignant tumor, and solid tumor; diseases accompanying angiopathy, suchas myocardial infarction, cerebral infarction, peripheral vascularatresia, angina pectoris, hypertension, hyperlipidemia, diabetes,diabetic retinopathy, glomerulonephritis, arteriosclerosis, thrombosis,hemolytic uremic syndrome, thrombotic thrombocytopenic purpa, ischemicheart disease, ischemic encephalopathy, heart failure, hemostasis, andchoroid circulatory dysfunction; diseases accompanying abnormal bonemetabolism, such as osteoporosis; diseases accompanying disorders ofinsulin-like growth factors or growth hormone action, such as dwarfism,acromegaly, and infantile chronic renal failure; diseases accompanyingabnormal differentiation or growth of smooth muscle cells, such asarteriosclerosis, bronchial disease, and restenosis; diseasesaccompanying abnormal differentiation or growth of skeletal musclecells, such as myasthenia gravis; diseases accompanying abnormal gastricacid secretion, such as gastric ulcer; and inflammatory diseasesaccompanying abnormal lymphocyte invasion, such as microbial infection,chronic hepatitis B, chronic rheumatoid arthritis, sepsis, graft versushost disease, insulin dependent diabetes mellitus, nephritis, traumaticbrain damage, inflammatory enteropathy, allergy, atopy, asthma,pollinosis, airway hypersensitivity, and autoimmune disease.

(5) Prevention and Treatment of Diseases by Suppressing Transcription orTranslation of DNAs Encoding a Protein of the Present Invention

Regarding the relationship between the rise in the expression level ofIGFBP and diseases, reports have been made that report the increases inIGFBP-2 and 3 as the cause of infantile chronic renal failure(Electroiyte Mrtab., 18, 320 (1992)); the rise in IGFBP-4 expression ofwomen fracture patients of old age accompanying rise in parathyroidhormone; the rise in IGFBP-7 and IGFBP-3 concentrations in thecerebrospinal fluid of leukemia patients (J. Clin. Endocrinol. Metab.,84, 1361 (1996)); in colon cancer, the rise in IGFBP-7 expression incolon cancer tissues and in colon cancer cell lines (J.Gastroenterology, 33, 213 (1998)); and the like.

Furthermore, IGFBP-7 is also thought to be involved in diseases relatingto bone metabolism and skeletal muscle differentiation due to the factsthat IGFBP-7 expression rises in relation to TGF-β and PTHPGE2(Endocrinology, 140, 1998 (1999)); that the expression of IGF-I issuppressed while IGFBP-7 expression is raised upon treatment ofosteoblasts with glucocorticoids (Endocrinology, 140, 228 (1999)); andthat IGFBP-7 has an effect on differentiation into skeletal muscles bysuppressing the differentiation promoting action of IGF (Exp. Cell Res.,237, 192 (1997), Endocrinology, 141, 100 (2000)).

Therefore, lowering the level of transcription or translation of DNAsencoding a protein of the present invention is useful for prevention andtreatment of diseases wherein the increase in the expression level of anIGFBP gene, or enhancement of IGFBP function is one of the causes.Furthermore, even in diseases that are not directly caused by IGFBP, theabove-mentioned diseases can be prevented or treated by symptomatictreatment by lowering the level of transcription or translation of genesencoding a protein of the present invention, or by suppressing thefunction of the protein of the present invention.

Furthermore, a patient whose physiological action via receptors areenhanced due to the increase of IGFBP expression, the physiologicalaction can be suppressed by administering a DNA, oligonucleotide, orderivative thereof of the present invention to the patient. Moreover,even if the increase in IGFBP expression is not the direct cause of thedisease, the DNAs, oligonucleotides, or derivatives thereof of thepresent invention can function as effective preventive agents andtherapeutic agents against diseases that are treatable by symptomatictreatment wherein the DNAs, oligonucleotides, or derivatives thereof ofthe present invention are administered.

Methods for using the preventive agents or therapeutic agents include,for example, antisense RNA/DNA techniques (Bioscience and Industry, 50,322 (1992); Kagaku, 46, 681 (1991); Biotechnology, 9, 358 (1992); Trendsin Biotechnology, 10, 87 (1992); Trends in Biotechnology 10, 152 (1992);Saibo Kogaku 16, 1463 (1997)); triple helix techniques (Trends inBiotechnology, 10, 132 (1992)); ribozyme techniques (Current Opinion inChemical Biology, 3, 274 (1999); FEMS Microbiology Reviews, 23, 257(1999); Frontiers in Bioscience, 4, D497 (1999); Chemistry & Biology, 6,R33 (1999); Nucleic Acids Research, 26, 5237 (1998); Trends InBiotechnology, 16, 438 (1998)); and decoy DNA methods (NipponRinsho—Japanese Journal of Clinical Medicine, 56, 563 (1998);Circulation Research, 82, 1023 (1998); Experimental Nephrology, 5, 429(1997); Nippon Rinsho—Japanese Journal of Clinical Medicine, 54, 2583(1996)). Using these methods, the expression of an arbitrary gene can besuppressed.

When using the DNAs, oligonucleotides, or derivatives thereof of thepresent invention as the above-mentioned preventive agents andtherapeutic agents, the DNAs, oligonucleotides, or derivatives thereofof the present invention themselves or those inserted into appropriatevectors, such as retrovirus vector, adenovirus vector, andadenovirus-associated virus vector, can be made into formulations;prescribed; and administered according to conventional methods mentionedbelow.

A vector for gene therapy inserted into virus vectors, such asretroviruses and adenoviruses, or other vectors for gene therapy, whichis used as an agent or preventive agent for gene therapy, can beproduced by formulating the vector for gene therapy and a base agent forgene therapy (Nature Genet., 8, 42 (1994)).

Any base agent can be used for gene therapy so long as it can be usedfor conventional injections, and includes, for example, distilled water;salt solutions, such as sodium chloride and a mixture of sodium chlorideand inorganic salts; sugar solutions of mannitol, lactose, dextran,glucose, etc.; amino acid solutions of glycine, arginine, etc.; mixedsolutions comprising organic acid solution or salt solution, and glucosesolution. In addition, according to conventional methods, the injectioncan be prepared as solutions, suspensions, or dispersion solutionsusing, in addition to the base, auxiliary agents, such as agents forosmoregulation; agents for pH adjustment; vegetable oils, such as sesameoil and soybean oil; or surfactants, such as lecithin or non-ionicsurfactants. By operations such as powderization and freeze-drying,these injections can be prepared as formulations to be dissolved justbefore use. An agent for gene therapy of the present invention in theform of liquid can be directly used for treatment, and those in a solidform by dissolving just before use for gene therapy into theabove-mentioned base that is sterilized as necessary. Methods foradministration of an agent for gene therapy of the present inventioninclude a method for local administration so that the agent is absorbedinto the site to be treated within the patient.

Alternatively, a DNA can be transported to the desired site of treatmentby non-viral gene transfection methods.

Examples of non-viral gene transfection methods well known in the artare the calcium phosphate precipitation method (Virology, 52, 456-467(1973); Science, 209, 1414-1422 (1980)), the microinjection method(Proc. Natl. Acad. Sci. USA, 77, 5399-5403 (1980); Proc. Natl. Acad.Sci. USA, 77, 7380-7384 (1980); Cell, 27, 223-231 (1981); Nature, 294,92-94 (1981)), the liposome mediated membrane fusion-mediatedtransfection method (Proc. Natl. Acad. Sci. USA, 84, 7413-7417 (1987);Biochemistry, 28, 9508-9514 (1989); J. Biol. Chem., 264, 12126-12129(1989); Hum. Gene Ther., 3, 267-275, (1992); Science, 249, 1285-1288(1990); Circulation, 83, 2007-2011 (1992)), and the direct DNA importand receptor-mediated DNA transfection method (Science, 247, 1465-1468(1990); J. Biol. Chem., 266, 14338-14342 (1991); Proc. Natl. Acad. Sci.USA, 87, 3655-3659 (1991); J. Biol. Chem., 264, 16985-16987 (1989);BioTechniques, 11, 474-485 (1991); Proc. Natl. Acad. Sci. USA, 87,3410-3414 (1990); Proc. Natl. Acad. Sci. USA, 88, 4255-4259 (1991);Proc. Natl. Acad. Sci. USA, 87, 4033-4037 (1990); Proc. Natl. Acad. Sci.USA, 88, 8850-8854 (1991); Hum. Gene Ther., 3, 147-154 (1991)).

It has been reported in studies regarding tumors, that the liposomemediated membrane fusion-mediated transfection method enables localizedimport and expression of genes in the target tissues by directadministration of liposome preparations to the target tissue (Hum. GeneTher., 3, 399 (1992)).

(6) Prevention and Treatment of Diseases with Decreased Expression Levelor Function of a Protein of the Present Invention

Regarding the relationship between diseases and decreased expression ofIGFBP, it has been reported that IGFBP-5 expression is decreased inosteoporosis; increase of free IGF-I accompanying lowered IGFBP-3expression is observed in compensatory hypertrophy after nephrectomy andsmall intestine resection (Baillieres Clin. Endocrinol. Metab., 8, 165(1994)); IGFBP-1 expression is decreased in malignant tumors of theendometrium (Growth Regul., 3, 74 (1993)); LOH is observed at afrequency of 50% or more at the gene loci of IGFBP-7 on the humanchromosome and IGFBP-7 expression is found to be decreased in breastcancer tissues (Oncogene, 16, 2459 (1996)); IGFBP-7 expression isdecreased at the mRNA level in prostate cancer tissues, and IGFBP-7expression is not detected in malignant prostate cancer-derived celllines (J. Clin. Endocrinol. Metab., 83, 4355 (1998)); expression of mRNAof IGFBP-7 is lowered in large uterine leiomyoma sites compared toneighboring uterine smooth muscle cells and small uterine smooth muscleswherein the tumor volume is 120 cm³ or less (Am. J. Reprod. Immunol.,43, 53 (2000)); the 5′ upstream region of the IGFBP-7 gene ismethylated, and its expression level is decreased in mouse hepaticcancer cells induced by SV40T antigen (Biochem. Biophys. Res. Commun.,267, 109 (2000)); IGFBP-7 expression is decreased in kidney and at sitesof angiopathy in type I diabetes model due to the administration ofstreptozotocin (Diabetes, 45, S111 (1996); J. Diabetes & itsComplications, 12, 252 (1998)); lowered IGFBP-7 expression is observedat the protein level in coronary artery smooth muscle cells of type IIdiabetes patients (Diabetes, 46, 1627 (1997)); the expression level ofIGFBP-7 is decreased at the mRNA and protein levels when bovineartery-derived smooth muscle cells are cultivated in a glucose-richmedium (Diabetes, 46, 1627 (1997), Diabetologia, 41, 134 (1998));IGFBP-7 stimulates PGI2 production in vascular wall (Nature, 271, 549(1978)); and that the level of IGFBP in blood is decreased in hemolyticuremic syndrome (Lancet, 2, 871 (1978)), thrombotic thrombocytopenicpurpa (Lancet, 2, 748 (1979)), sickle cell anemia (Br. J. Haematol., 48,545 (1981)), acute myocardial infarction (Coronary, 2, 49 (1985)),diabetic angiopathy (Metabolism, 38, 837 (1989); Haemostasis, 16, 447(1986); Diab. Res. Clin. Pract., 3, 243 (1987)), and arteriosclerosis.

Furthermore, IGFBP-7 is also considered to be involved with diseasesrelating to bone metabolism and skeletal muscle differentiation, due tothe facts that IGFBP-7 expression rises in accordance to TGF-β andPTHPGE2 (Endocrinology, 140, 1998 (1999)), that IGF-I expression issuppressed while IGFBP-7 expression is raised upon treatment ofosteoblasts with glucocorticoids (Endocrinology, 140, 228 (1999)), andthat IGFBP-7 affects differentiation into skeletal muscles bysuppressing the differentiation-promoting action of IGF (Exp. Cell Res.,237, 192 (1997); Endocrinology, 141, 100 (2000)).

Due to the elongation of cell division time, decrease in colony formingability in soft agar medium, decrease of the tumor forming ability innude mouse transplantation, and elevation of apoptosis induction rate bydrug treatment occurring by the forced expression of IGFBP-7 in prostatecancer cell lines with decreased IGFBP-7 expression (Cancer Res., 59,2370 (1999)); and due to the fact that the forced expression of IGFBP-7in osteosarcoma cell lines, whose p53 function is lost, leads to thesuppression of proliferation of the cell lines similarly to thetransfection of the p53 gene (Oncogene, 12, 1361 (1996)), theabove-mentioned diseases associated with decreased IGFBP expression canbe prevented or treated by increasing the expression of a DNA andprotein of the present invention. Furthermore, even for diseases thatare not directly caused by IGFBP, the above-mentioned diseases for whichsymptomatic treatment is possible can be prevented or treated byincreasing the level of transcription or translation of a gene encodinga protein of the present invention, or by increasing the amount of aprotein of the present invention.

Therefore, the physiological action of a patient with decreasedphysiological action of a protein of the present invention due to thedecreased expression or function of IGFBP can be promoted byadministering a DNA, oligonucleotide or its derivative of the presentinvention or a protein of the present invention to the patient.Furthermore, the DNAs, oligonucleotides, or derivatives thereof of thepresent invention, or proteins of the present invention are useful aspreventive agents and therapeutic agents against the above-mentioneddiseases for which symptomatic treatment is possible, even if thedecreased expression or function of IGFBP is not the direct cause of thedisease.

As methods of administration of preventive agents and therapeuticagents, for example, a patient with decreased expression or mutation ofa protein of the present invention for whom normal physiological actionof the protein cannot be expected can be treated by (i) administering aDNA encoding the protein of the present invention to the patient andexpressing the DNA; (ii) inserting a DNA encoding the protein of thepresent invention into target cells, expressing the DNA, and thentransplanting the cells to the patient; or (iii) administering theprotein of the present invention to the patient, to increase the amountof the protein of the present invention in the patient and letsufficiently exert the physiological function of the protein. Therefore,the DNAs encoding the proteins of the present invention or the proteinsof the present invention are useful as safe preventive and therapeuticagents with low toxicity against diseases associated with decreasedexpression level or function of a protein of the present invention,diseases that result from functional abnormality due to mutations ofthis protein, or diseases that are not directly caused by the protein ofthe present invention but can be treated by symptomatic treatment by theadministration of the protein or a DNA encoding the protein of thepresent invention.

To use a DNA encoding a protein of the present invention as theabove-mentioned preventive agents and therapeutic agents, the DNA of thepresent invention alone or the DNA inserted into appropriate vectors,such as retrovirus vector, adenovirus vector, and adenovirus associatedvirus vector, can be formulated, prescribed, and administered followingthe conventional methods of (5) mentioned above.

In addition, medicaments containing a protein of the present inventionas their active ingredient can be administered as the active ingredientalone, but ordinarily, they are mixed with one or more pharmacologicallyacceptable carriers, and are preferably provided as a medicalpreparation produced by arbitrary methods well known in the art ofpharmaceutical technology. Preferably, water, or sterilized solutionsdissolved in water-based carriers, such as aqueous solutions of sodiumchloride, glycine, glucose, and human albumin, are used.Pharmacologically acceptable additives, such as buffer agents andisotonic agents may be added to provide a physiological condition forthe pharmaceutical solution, including sodium acetate, sodium chloride,sodium lactate, potassium chloride, sodium citrate, and so on.Furthermore, the medicament can be stored after freeze-drying and can beused upon dissolving in an appropriate solvent at the time of use.

The most effective administration route is preferably adopted fortreatment, and includes oral administration or parenternaladministration, such as oral, tracheobronchial, endorectal,subcutaneous, intramuscular, or intravenous administration. Examples ofadministration forms are propellants, encapsulated formulations,tablets, granules, syrups, emulsions, suppositories, injections,ointments, tapes, and the like.

Pharmaceutical preparations suitable for oral administration areemulsions, syrups, encapsulated formulations, tablets, powders,granules, and the like. For example, liquid preparations such asemulsions and syrups can be produced using water; sugars, such assucrose, sorbitol, and fructose; glycols, such as polyethylene glycoland propylene glycol; oils, such as sesame oil, olive oil, and soybeanoil; preservatives, such as p-hydroxybenzoic acid esters; flavors, suchas strawberry flavor and peppermint; and the like, as additives.Encapsulated formulations, tablets, powders, granules, and the like canbe produced using additives including excipients, such as lactose,glucose, sucrose, and mannitol; disintegrators, such as starch andsodium alginate; lubricants, such as magnesium stearate and talc;binders, such as polyvinyl alcohol, hydroxypropyl cellulose, andgelatin; surfactants, such as fatty acid esters; plasticizers, such asglycerin.

Examples of pharmaceutical preparations suitable for parenternaladministration are injections, suppositories, propellant, and the like.For example, injections are prepared using carriers and the likecomprising salt solutions, glucose solutions, or mixtures of both.Suppositories are prepared using carriers such as cacao butter,hydrogenated fat, or carboxylic acids. Furthermore, propellants areprepared using a protein of the present invention itself, or withcarriers that do not stimulate the oral and respiratory tract mucosa ofthe recipient and which facilitate the absorbance by dispersing theprotein as fine particles. More specifically, examples of carriers arelactose, glycerin, and the like. Depending on the characteristic of theused protein and the carriers, pharmaceutical preparations such asaerosols and dry powders are possible. In addition, components cited asexamples of additives for oral agents may be added to these parenternalagents.

Although the dose or frequency of administration varies depending on thedesired therapeutic effect, administration method, therapeutic duration,age, weight, and the like, it is usually 10 μg/kg to 8 mg/kg per day foran adult.

(7) Methods for Obtaining the Promoter Regions of the Genes Encoding theProteins of the Present Invention

Using the DNAs or oligonucleotides of the present invention as a probe,the promoter regions of the genes can be obtained using known methods(New Cell Engineering Experiment Protocols, University of TokyoInstitute of Medical Science Division of Anti-Cancer Research edition,Shujun-sha (1993)).

The promoter regions include all promoter regions involved in thetranscription of the genes encoding the proteins of the presentinvention in mammalian cells. Such examples are the promoter regionsinvolved in the transcription of a gene encoding a protein of thepresent invention in the small intestine of human. The promoters can beused for methods of screening for substances that regulate thetranscription or translation of the DNAs encoding the proteins of thepresent invention, which will be mentioned below in (8).

(8) Methods of Screening for Substances that Regulate the Transcriptionor Translation of the Genes Encoding the Proteins of the PresentInvention

Proteins belonging to the IGFBP superfamily have been reported to beassociated with diseases, for example, diseases accompanying abnormalcell growth, such as acute myelogenous leukemia, breast cancer, prostatecancer, colon cancer, hepatic cancer, myeloma, uterine leiomyoma,malignant tumor, and solid tumor; diseases accompanying angiopathy, suchas myocardial infarction, cerebral infarction, peripheral vascularatresia, angina pectoris, hypertension, hyperlipidemia, diabetes,diabetic retinopathy, glomerulonephritis, arteriosclerosis, thrombosis,hemolytic uremic syndrome, thrombotic thrombocytopenic purpa, ischemicheart disease, ischemic encephalopathy, heart failure, hemostasis, andchoroid circulatory dysfunction; diseases accompanying abnormal bonemetabolism, such as osteoporosis; diseases accompanying disorders ofinsulin-like growth factors or growth hormone action, such as dwarfism,acromegaly, and infantile chronic renal failure; diseases accompanyingabnormal differentiation or growth of smooth muscle cells, such asarteriosclerosis, bronchial disease, and restenosis; diseasesaccompanying abnormal differentiation or growth of skeletal musclecells, such as myasthenia gravis; diseases accompanying abnormal gastricacid secretion such as gastric ulcer; and inflammatory diseasesaccompanying abnormal lymphocyte invasion, such as microbial infection,chronic hepatitis B, chronic rheumatoid arthritis, sepsis, graft versushost disease, insulin dependent diabetes mellitus, nephritis, traumaticbrain damage, inflammatory enteropathy, allergy, atopy, asthma,pollinosis, airway hypersensitivity, and autoimmune disease.

Changes in the transcription level of DNAs encoding IGFBPs or functionalchanges of the proteins of the present invention can be considered as afactor for the above-mentioned diseases. In this case, regulation of thelevel of transcription or translation of the DNAs encoding the proteinsof the present invention is effective for the prevention and treatmentof such diseases. Diseases that are not directly caused by IGFBP can bealso prevented or treated by symptomatic treatment of theabove-mentioned diseases by regulating the level of transcription ortranslation of the genes encoding the proteins of the present invention.

Therefore, compounds that promote or suppress the process oftranscription of the genes encoding the proteins of the presentinvention, or the process of translation from the transcription productsto the proteins can regulate the cell functions exerted through theproteins by regulating the expression of the proteins, and is useful assafe pharmaceutical compositions with low toxicity.

The compounds can be obtained by the following methods of (a) to (c):

-   -   (a) A method for selecting and obtaining compounds that have the        activity to increase or decrease the amount of a protein of the        present invention by [i] culturing cells expressing the protein,        or [ii] culturing cells expressing the protein in the presence        of a test substance for 2 hours to 1 week by the culturing        method of the above-mentioned 2, and then measuring and        comparing the amount of the protein in the cells using the        antibodies of the present invention according to (3) mentioned        above.

Examples of the methods for measurement using the antibodies of thepresent invention include ELISA method using microtiter plates,fluorescent antibody method, Western Blotting method, detection methodsutilizing immunohistological staining method, and the like.

-   -   (b) A method for selecting and obtaining compounds that have the        activity to increase or decrease the amount of a transcription        product of DNA encoding a protein of the present invention by        [i] culturing cells expressing the protein or [ii] culturing        cells expressing the protein in the presence of a test substance        for 2 hours to 1 week by the above-mentioned cultivation method        of 2, and then measuring and comparing the amount of        transcription product of the DNA encoding the protein in the        cell using methods such as the Northern hybridization method or        PCR method mentioned in above (1).    -   (c) First, a plasmid containing DNA wherein a reporter gene is        connected downstream of a promoter obtained in the        above-mentioned (7) by conventional methods, and then the        plasmid is inserted into animal cells according to the        above-mentioned method of 2 to obtain a transformant. A method        for selecting and obtaining compounds that have the activity to        increase or decrease the expression level of the reporter gene        by [i] culturing the transformant or [ii] culturing the        transformant in the presence of a test substance for 2 hours to        1 week by the above-mentioned culturing method of 2, and then        measuring and comparing the expression level of the reporter        gene in the cell by conventional methods (“Shin Saibokogaku        Jikken Purotokoru (New Cell Engineering Experiment Protocols)”,        University of Tokyo Institute of Medical Science Division of        Anti-Cancer Research edition, Shujun-sha (1993); Biotechniques,        20, 914 (1996); J. Antibiotics, 49, 453 (1996); Trends in        Biochemical Sciences, 20, 448 (1995); Saibo Kogaku (Cell        Engineering), 16, 581 (1997)).

Examples of reporter genes are, chloramphenicol acetyltransferase gene,β-galactosidase gene, β-lactamase gene, luciferase gene, greenfluorescent protein (GFP) gene, and the like.

(9) Methods of Screening for Substances that Regulate the Function ofthe Proteins of the Present Invention

Proteins belonging to the IGFBP superfamily have been reported to beassociated with diseases, for example, diseases accompanying abnormalcell growth, such as acute myelogenous leukemia, breast cancer, prostatecancer, colon cancer, hepatic cancer, myeloma, uterine leiomyoma,malignant tumor, and solid tumor; diseases accompanying angiopathy, suchas myocardial infarction, cerebral infarction, peripheral vascularatresia, angina pectoris, hypertension, hyperlipidemia, diabetes,diabetic retinopathy, glomerulonephritis, arteriosclerosis, thrombosis,hemolytic uremic syndrome, thrombotic thrombocytopenic purpa, ischemicheart disease, ischemic encephalopathy, heart failure, hemostasis, andchoroid circulatory dysfunction; diseases accompanying abnormal bonemetabolism, such as osteoporosis; diseases accompanying disorders ofinsulin-like growth factors or growth hormone action, such as dwarfism,acromegaly, and infantile chronic renal failure; diseases accompanyingabnormal differentiation or growth of smooth muscle cells, such asarteriosclerosis, bronchial disease, and restenosis; diseasesaccompanying abnormal differentiation or growth of skeletal musclecells, such as myasthenia gravis; diseases accompanying abnormal gastricacid secretion, such as gastric ulcer; and inflammatory diseasesaccompanying abnormal lymphocyte invasion, such as microbial infection,chronic hepatitis B, chronic rheumatoid arthritis, sepsis, graft versushost disease, insulin dependent diabetes mellitus, nephritis, traumaticbrain damage, inflammatory enteropathy, allergy, atopy, asthma,pollinosis, airway hypersensitivity, and autoimmune disease.

Change in the function of IGFBP is thought to be a cause of theabove-mentioned diseases, and in such a case, inhibition or enhancementof the function of a protein of the present invention is effective forpreventing and treating the diseases. Furthermore, even for diseasesthat are not directly caused by IGFBPs, the above-mentioned diseases canbe prevented or treated by symptomatic treatment by inhibiting orenhancing the function of a protein of the present invention.

Therefore, a patient whose physiological action of cells is decreased orenhanced due to the change in the function of a protein of the presentinvention, the physiological action can be regulated by administering acompound of the present invention that regulate the function.Furthermore, even if the change in the function of a protein of thepresent invention is not the direct cause of a disease, diseases forwhich symptomatic treatment is possible by regulating the function of aprotein of the present invention can also be prevented or treated byadministering the compound.

Such compounds can be obtained, for example, by the following methods:

-   -   a method for selecting and obtaining compounds that have the        activity to inhibit or enhance the function of a protein of the        present invention by culturing [i] cells that express the        protein and [ii] in the presence of a test substance for 2 hours        to 1 week by the above-mentioned culturing method of 2, and then        detecting and comparing cellular responses caused by the fact        that the protein has functioned.

Methods for detecting cellular responses that occur due to the functionof a protein of the present invention are, for example, conventionalmethods for detecting changes of intracellular information transfer,transcription of genes, uptake of sugars, or growth that is dependent oninsulin or insulin-like growth factors contained in a culture medium.Further examples are conventional methods for measuring changes inprostaglandin production (Nature, 263, 663, (1976); Rinsho Kagaku(Clinical Science), 17, 958, (1981)).

(10) Methods of Screening for Substances that Specifically Bind to aProtein of the Present Invention

Substances that specifically bind to a protein of the present inventioncan be used to develop preventive agents or therapeutic agents fordiseases caused by the protein of the present invention. A receptor of aprotein of the present invention binds to the protein of the presentinvention, and has the function to transmit information into cells andexerts physiological action. Therefore, similarly to substancesregulating the proteins of the present invention, or transcription ortranslation of genes encoding the proteins of the present invention, forexample, substances regulating the activity of the receptor, or thetranscription or translation of genes encoding the receptor are usefulas preventive agents and therapeutic agents for diseases caused byfunctional abnormality of the protein of the present invention. Examplesof these substances are low-molecular-weight compounds, proteins, andthe like.

Methods for screening proteins that specifically bind to a protein ofthe present invention are, for example, methods for expression cloningaccording to Molecular Cloning, 2nd edition; Current Protocols inMolecular Biology; Science, 255, 989 (1992); and the like. A proteinspecifically binding to the protein of the present invention can beobtained by selecting, from test samples, proteins that bind to theprotein of the present invention when the protein and the test sampleare brought in contact.

Specifically, the following methods can be mentioned.

cDNAs are prepared from tissues. Recombinant vectors are constructed byinserting each of the cDNAs downstream of a promoter in an appropriateexpression vector to prepare a cDNA library. Transformants expressingone of the genes expressed in the tissues are obtained by transfectingthe recombinant vectors into host cells suitable for the expressionvector. Transformants that produce proteins that specifically bind to alabeled protein of the present invention are selected.

Proteins that specifically bind to the protein of the present inventioncan be obtained by determining the genetic sequence encoded by the cDNAintroduced into the selected transformant.

Examples of methods for preparing a cDNA library include theabove-mentioned methods of 1.

Methods for introducing recombinant vectors, methods for obtainingtransformants, and methods for culturing the obtained transformants in amedium are exemplified by the above-mentioned methods of 2.

By co-culturing the transformant and a protein of the present inventionthat is labeled by a conventional method for labeling proteins, such asradioactive isotope labeling and biotinylation, transformants producinggene products that specifically bind to the labeled protein of thepresent invention can be selected.

Examples of methods for isolating cDNAs introduced to the selectedtransformants include the Hirt method or methods for collecting phagevectors and plasmid vectors according to Molecular Cloning, 2nd edition;Current Protocols in Molecular Biology; Mol. Cell. Biol., 8, 2837(1988); and the like.

Examples of methods for determining the genetic sequences of theisolated cDNAs include the above-mentioned methods of 1.

(11) Medicaments Containing Antibodies of the Present Invention

The antibodies of the present invention are useful as preventive agentsand therapeutic agents of diseases relating to a protein of the presentinvention.

Regarding the relationship between the rise in the expression level ofIGFBPs and diseases, it has been reported that increase in IGFBP-2 and 3is the cause of infantile chronic renal failure (Electroiyte Mrtab., 18,320 (1992)); IGFBP-4 expression rises in women fracture patients of oldage accompanying rise in parathyroid hormone; IGFBP-7 and IGFBP-3concentrations in cerebrospinal fluid rise in leukemia patients (J.Clin. Endocrinol. Metab., 84, 1361 (1996)); in colon cancer, IGFBP-7expression rises in colon cancer tissues and in colon cancer cell lines(J. Gastroenterology, 33, 213 (1998)); and the like.

Furthermore, IGFBP-7 is thought to be also involved in diseases relatingto bone metabolism and skeletal muscle differentiation due to the factsthat IGFBP-7 expression rises in response to TGF-β, PTH, and PGE2(Endocrinology, 140, 1998 (1999)); that IGF-I expression is suppressedwhile IGFBP-7 expression is raised upon treatment of osteoblasts withglucocorticoids (Endocrinology, 140, 228 (1999)); and that IGFBP-7 hasan effect on differentiation into skeletal muscles by suppressing thedifferentiation promoting action of IGF (Exp. Cell Res., 237, 192(1997), Endocrinology, 141, 100 (2000)).

Therefore, regarding diseases wherein the increase in the expressionlevel or enhancement of the function of IGFBP is one of the causes,suppressing the expression levels or inhibiting the function of aprotein of the present invention is effective for prevention andtreatment of the diseases. Even in diseases that are not directly causedby IGFBPs, the above-mentioned diseases for which symptomatic treatmentsare possible can be prevented or treated by suppressing the expressionlevel or by inhibiting the function of the protein of the presentinvention.

Accordingly, a patient with enhanced physiological action of cells dueto the increase in the expression level or functional enhancement ofIGFBPs, the physiological action can be suppressed by administering anantibody that binds to a protein of the present invention. Furthermore,even when the proteins of the present invention are not the direct causeof the diseases, by suppressing the function of the proteins of thepresent invention, diseases for which symptomatic treatments arepossible can be prevented and treated by administering an antibody ofthe present invention.

Medicaments containing an antibody as an active ingredient can beprovided as pharmaceutical preparations produced by arbitrary methodswell known in the art of pharmacological technology, following theabove-mentioned methods for producing medicaments containing a proteinof the present invention according to (6).

(12) Medicaments Containing a Compound Obtained by the Screening Methodof (8)

Compounds that promote or suppress the process of transcription of agene encoding a protein of the present invention, or the process oftranslation from a transcription product to the protein are useful assafe pharmaceutical compositions having low toxicity.

Compounds obtained in (8) can be provided as pharmaceutical preparationsproduced by arbitrary methods well known in the art of pharmacologicaltechnology, following the methods for producing medicaments containing aprotein of the present invention according to (6).

(13) Production of Non-Human Knockout Animals Using a DNA of the PresentInvention

Using recombinant vectors comprising a DNA of the present invention,mutant clones, wherein a gene on chromosome encoding a protein of thepresent invention is inactivated or substituted with an arbitrarysequence by known methods for homologous recombination (for example,Nature 326, 6110, 295 (1987); Cell, 51, 3, 503 (1987)), are produced inembryonic stem cells of desired non-human animals, such as cattle,sheep, goat, pig, horse, mouse, and chicken (for example, Nature 350,6315, 243 (1991)). Using a mutant clone of embryonic stem cell, achimeric individual consisting of the embryonic stem cell clone andnormal cell can be prepared by methods, such as the aggregation chimeramethod or the infusion chimera method against blastocysts of fertilizedeggs of animals. By crossing the chimeric individual and a normalindividual, individuals having an arbitrary mutation on the geneencoding the protein of the present invention located on chromosomes ofall cells of the entire body can be obtained. Furthermore, a non-humanknockout animal wherein the expression of the gene encoding the proteinof the present invention is partially or completely suppressed can beobtained from homomeric individuals that have mutations on both of thehomologous chromosomes by crossing such individuals.

Alternatively, non-human knockout animals can be produced by introducingmutations to arbitrary positions of a gene on chromosome encoding aprotein of the present invention. For example, the activity of a geneproduct can be altered by introducing mutations by substitutions,deletions, insertions, and the like of bases in the coding region of thegene on chromosome encoding a protein of the present invention.Furthermore, the degree, timing, tissue specificity and the like, of theexpression of the gene can be altered by introducing similar mutationsto its expression regulatory region. Furthermore, combination with aCre-loxP system enables a more active regulation of the timing ofexpression, expression site, expression level, and the like. An example,using a promoter expressed in a specific region of the brain, wherein adesired gene was deleted only in that region (Cell, 87, 7, 1317 (1996)),and an example wherein a desired gene was deleted organ-specifically atthe desired timing using adenoviruses expressing Cre (Science, 278,5335, (1997)) are known as such examples.

Therefore, for a gene on chromosome encoding a protein of the presentinvention, a non-human knockout animal wherein the expression isregulated at arbitrary timing and in arbitrary tissues in this manner,or have arbitrary insertions, deletions, and substitutions in theircoding regions or expression regulatory regions can be produced.

The non-human knockout animals can induce symptoms of various diseasescaused by the protein of the present invention at arbitrary timing, toarbitrary degrees, or at arbitrary sites.

Thus, the non-human knockout animals of the present invention serve asextremely useful animal models for the treatment and prevention ofvarious diseases caused by the protein of the present invention. Inparticular, they are very useful as models for the evaluation oftherapeutic agents and preventive agents against such diseases, as wellas functional foods, health foods and the like.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the comparison of the amino acid sequences of C-hsi13412and C-kaia397 (SEQ ID NO:35), and IGFBP family factors (SEQ IDNOS:28-34).

FIG. 2 shows the hydrophobicity plot of the protein encoded byC-hsi13412.

FIG. 3 shows the hydrophobicity plot of the protein encoded byC-kaia397.

FIG. 4 shows the comparison of C-hsi13412 and C-kaia397, and the humangenome sequence.

FIG. 5 shows the expression of C-hsi13412 and C-kaia397 by RT-PCR. Thefirst panel shows the expression in each tissue, and the second panelshows the result of analyzing the expression in each cell line.

FIG. 6 shows the expression of C-hsi13412 and C-kaia397 by RT-PCR.

FIG. 7 shows the expression of the proteins encoded by C-hsi13412 andC-kaia397 using COS-1 cells as hosts. Mock represents the control.

FIG. 8 shows the expression and glycosylation of the proteins encoded byC-hsi13412 and C-kaia397 using CHO cells as hosts. In the figure, Vindicates CHO/mock cell line, C-kaia397 indicates CHO/C-kaia397 cellline, C-kaia397h indicates CHO/C-kaia397h cell line, C-hsi13412indicates CHO/C-hsi13412 cell line, and C-hsi13412h indicatesCHO/C-hsi13412h cell line. In addition, + indicates the addition ofglycosidase, − indicates the addition of 100 mmol/l of Tris-HCl buffer(pH 7.5) instead of glycosidase, and n indicates cases without enzymereaction.

FIG. 9 shows the expression of proteins encoded by C-hsi13412 andC-kaia397 using insect cells as hosts. Mock represents the control.

FIG. 10 shows the analysis by Western Blotting using polyclonalantibodies that recognize the proteins encoded by C-hsi13412 andC-kaia397. In the figure, the blotting on the right shows the resultswith antibodies that were obtained using compound 2 as its immunogen,and the blotting figure on the left shows the result with antibodiesthat were obtained using compound 3 as its immunogen.

FIG. 11 shows the detection by Western Blotting of proteins encoded byC-hsi13412 or C-kaia397 using KM2961 and KM2962. Mock represents thecontrol.

FIG. 12 shows the binding affinity of IGF to the proteins encoded byC-hsi13412 or C-kaia397 by far-Western. Mock represents the control.

FIG. 13 shows the effect of the protein encoded by C-hsi13412 orC-kaia397 on IGF-dependent growth of HT-29 cells. Mock represents thecontrol.

FIG. 14 shows the binding activity against the antigenic peptides ofKM2961 and KM2962. In the figure, KM2961 represents the culturesupernatant obtained by cultivating, for 3 days, hybridomas that producethe monoclonal antibody KM2961; and KM2961 represents the culturesupernatant obtained by cultivating, for 3 days, hybridomas that producethe monoclonal antibody KM2961. The white columns show the reactionsbetween these culture supernatants and plates that are not coated withthe antigenic peptides, and the black and shaded columns show thereactions of these culture supernatants with plates coated with theantigenic peptides.

BEST MODE FOR CARRYING OUT THE INVENTION

The present invention is illustrated in detail below with reference toExamples. However, these Examples are only provided for illustrations ofthe present invention, and the present invention should not to beconstrued as being limited thereto.

Example 1 Preparation of Human Small Intestinal Mucosal Tissue-DerivedcDNA Library

According to the method described in Molecular Cloning, 2nd edition,mRNAs were extracted from human small intestinal mucosal tissues. Then,polyA(+)RNAs were purified using oligo dT cellulose. cDNA library wasprepared by the oligo-capping method (Gene, 138, 171 (1994)) from thepolyA(+)RNAs. Using oligo-cap linker (SEQ ID NO: 5) and oligo dT primer(SEQ ID NO: 6), BAP (Bacterial Alkaline Phosphatase) treatment, TAP(Tobacco Acid Phosphatase) treatment, RNA ligation, synthesis of primarystrand cDNAs, and removal of RNAs were carried out according to themethod described in the literatures (Protein, Nucleic Acid, and Enzyme,41, 197 (1996); Gene, 200, 149 (1997)). Next, conversion todouble-stranded cDNAs was carried out by PCR (polymerase chain reaction)using 5′ end sense primer (SEQ ID NO: 7) and 3′ end antisense primer(SEQ ID NO: 8) as PCR primers, and then the double stranded cDNAs weredigested with SfiI. The PCR was carried out using a commerciallyavailable kit, Geneamp XL PCR kit (Perkin Elmer), by heating at 95° C.for 5 minutes, then repeating 12 reaction cycles of 95° C. for 1 minute,58° C. for 1 minute, and 72° C. for 10 minutes, and then maintaining thetemperature at 4° C. Then, recombinant DNAs were produced by linking thecDNAs in a fixed direction to pME18SFL3 vectors (GeneBank AB009864,Expression vector, 3392 bp) digested with DraIII, to prepare a cDNAlibrary by transforming Escherichia coli DH5α with the recombinant DNAs.A sequencing reaction of the nucleotide sequences of the 5′ end and 3′end of cDNAs of each plasmid DNA harbored by the obtained transformantswas performed using DNA sequencing reagent (Dye Terminator CycleSequencing FS Ready Reaction Kit, dRhodamine Terminator Cycle SequencingFS Ready Reaction Kit or BigDye Terminator Cycle Sequencing FS ReadyReaction Kit, PE Biosystems) according to the instructions, to determineits sequence with DNA sequencer (ABI PRISM 377, PE Biosystems).

Example 2 Identification of Novel Proteins Belonging to the IGFBPSuperfamily

Using 10 molecules, human IGFBP-1, human IGFBP-2, human IGFBP-3, humanIGFBP-4, human IGFBP-5, human IGFBP-6, human IGFBP-7, human IGFBP-8,human IGFBP-9, and human IGFBP-10 as known proteins that belong to theIGFBP superfamily, registered in protein amino acid database SWISSPROTor nucleotide sequence database GenBank, 2 types of clones, C-hsi13412and C-kaia397, having amino acid sequence homology to these moleculeswere selected from the nucleotide sequences of each clone of theproduced cDNA library. The amino acid sequence of C-hsi13412 is shown inSEQ ID NO: 1, and its nucleotide sequence is shown in SEQ ID NO: 3. Theamino acid sequence of C-kaia397 is shown in SEQ ID NO: 2, and itsnucleotide sequence is shown in SEQ ID NO: 4.

In homology analysis using BLAST2, the amino acid sequence of C-hsi13412showed a significant homology of 38% with a P-value of 4.0×10⁻⁴¹ tohuman IGFBP-7 (accession number: I52825), which is a protein belongingto the insulin-like growth factor binding protein family. Similarly, inthe homology analysis using BLAST2, the amino acid sequence of C-kaia397showed a significant homology of 35% with a P-value of 2×10⁻¹⁶ to humanIGFBP-7, which is a protein belonging to the insulin-like growth factorbinding protein family. In the insulin-like growth factor bindingprotein superfamily, there are 10 cysteine residues that are importantfor the binding to IGF-1, IGF-2, and insulin; and mainly theinsulin-like growth factor binding motif (GCGCCXXC (SEQ ID NO:26) (G:glycine residue, C: cysteine residue, X: arbitrary amino acid residue))containing 4 of these cysteine residues are highly conserved amongfactors belonging to this family. According to a comparison of the aminoacid sequences of C-hsi13412 and C-kaia397 with each factor of theinsulin-like growth factor binding protein superfamily, it was revealedthat the position and number of cysteine residues are also conserved andthe insulin-like growth factor binding motif region was also found to behighly conserved in the amino acid sequences shown in SEQ ID NOS: 1 and2 (FIG. 1). Furthermore, the signal sequence at the N-terminus was alsohighly conserved in C-hsi13412, C-kaia397, and among factors belongingto the insulin-like growth factor binding protein superfamily (FIG. 1).

According to the above-mentioned results, C-hsi13412 and C-kaia397 wererevealed to be novel proteins belonging to the insulin-like growthfactor binding protein superfamily that have the activity of theinsulin-like growth factor binding protein superfamily. IGFBP-1,IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, and IGFBP-7 in FIG. 1represent human IGFBP-1, human IGFBP-2, human IGFBP-3, human IGFBP-4,human IGFBP-5, human IGFBP-6, and human IGFBP-7, respectively.

Furthermore, the amino acid sequence conserved among C-hsi13412,C-kaia397, and the insulin-like growth factor binding proteinsuperfamily are shown in white on a dark background, and the cysteineresidues that are highly conserved within the family are shown with anasterisk.

By searching nucleotide sequence databases, GenBank/EMBL/DDBJ, for thenucleotide sequence shown in SEQ ID NO: 3 using BLAST2, 2 nucleotidesequences that seemed to be ESTs derived from the same gene as thenucleotide sequence encoding the amino acid of C-hsi13412 was found tomatch to the sequence. The GenBank accession numbers of these ESTs areA1667734 and R30743. Similarly, by searching nucleotide sequencedatabases, GenBank/EMBL/DDBJ, for the nucleotide sequence shown in SEQID NO: 4 using BLAST2, 1 nucleotide sequence that seemed to be an ESTderived from the same gene as the nucleotide sequence encoding the aminoacid of C-kaia397 was found to match to the sequence. The GenBankaccession number of this EST is A1667734. These EST nucleotide sequencesdo not cover the entire length of C-hsi13412 and C-kaia397. Furthermore,although genes showing homology to the nucleotide sequences of each ESTwere searched in each of the databases of GenBank, EMBL, and DDBJ usingBLAST2, the ESTs did not show a significant homology to the insulin-likegrowth factor binding protein superfamily. Therefore, C-hsi13412 andC-kaia397 were revealed to be novel genes obtained for the first time bythe present invention.

Escherichia coli harboring the plasmid C-hsi13412 that contains a cDNA(entire nucleotide sequence shown in SEQ ID NO: 3) encoding the proteinhaving the amino acid sequence shown in SEQ ID NO: 1: Escherichia coliDH5α/pME18SFL3-C-hsi13412, and Escherichia coli harboring the plasmidC-kaia397 that contains a cDNA (entire nucleotide sequence shown in SEQID NO: 4) encoding the protein having the amino acid sequence shown inSEQ ID NO: 2: Escherichia coli DH5α/pME18SFL3-C-kaia397 have beendeposited under the accession numbers, FERM BP-7181 and FERM BP-7180,respectively, in the National Institute of Advanced Industrial Scienceand Technology, Patent Microorganism Depository, AIST Tsukuba Central 6,1-1-1 Higashi, Tsukuba, Ibaraki, Japan (postal code 305-8366) (old name:National Institute of Bioscience and Human-Technology, NationalInstitute of Advanced Industrial Science and Technology; old address:1-1-3 Higashi, Tsukuba, Ibaraki, Japan 305-8566) on Jun. 2, 2000.

Example 3 Analysis of the Nucleotide Sequences of C-hsi13412 andC-kaia397

Based on the nucleotide sequences of C-hsi13412 set forth in SEQ ID NO:3 and C-kaia397 set forth in SEQ ID NO: 4, sequences in the vicinity ofthe initiation codon were analyzed using an initiation codon predictionprogram ATGPr for proteins (Bioinformatics, 14, 384 (1998)). ATG atpositions 177 to 179 was specified as the initiation codon, and TAG atpositions 1089 to 1091 was specified as the stop codon, and the proteinencoded in the ORF was estimated to be composed of 304 amino acids forC-hsi13412. ATG at positions 926 to 928 was specified as the initiationcodon, and TAG at positions 1517 to 1519 was specified as the stopcodon, and the protein encoded in the ORF was estimated to be composedof 197 amino acids for C-kaia397. By mapping hydrophobicity plots usingGENETYX-MAC 7.3 (SOFTWARE DEVELOPMENT CO., LTD) based on the amino acidsequences encoded by C-hsi13412 set forth in SEQ ID NO: 1 and C-kaia397set forth in SEQ ID NO: 2, a highly hydrophobic region characteristic ofsecretory proteins existed in the N-terminal regions of both genes (FIG.2, FIG. 3).

Example 4 Analysis of Genomic Genes of C-hsi13412 and C-kaia397

Based on the nucleotide sequences of C-hsi13412 set forth in SEQ ID NO:3 and C-kaia397 set forth in SEQ ID NO: 4, nucleotide sequenceinformation of the human genome DNA region containing both nucleotidesequences shown in SEQ ID NO: 3 and SEQ ID NO: 4 was obtained usingGenomeWalker™ kits (Clontech) according to the attached instructions.Furthermore, searching the nucleotide sequence databasesGenBank/EMBL/DDBJ using BLAST2, nucleotide sequence information ofgenomic clone AL133215 containing both of the nucleotide sequences shownin SEQ ID NO: 3 and SEQ ID NO: 4 was obtained.

According to a comparison of the obtained human genomic sequence withthe nucleotide sequence shown in SEQ ID NO: 3 and the nucleotidesequence shown in SEQ ID NO: 4, the nucleotide sequence shown in SEQ IDNO: 3 was found to exist separated in 5 parts, and the nucleotidesequence shown in SEQ ID NO: 4 in 7 parts over a span of approximately 7kb on same human genome, chromosome 10 (FIG. 4). The protein having theamino acid sequence shown in SEQ ID NO: 1 is composed of 5 exons, andthe protein having the amino acid sequence shown in SEQ ID NO: 2 iscomposed of 7 exons, and the 2nd and 4th exons completely coincided.Furthermore, differences observed in the comparison of amino acidsequences shown in SEQ ID NOS: 1 and 2 were found to arise due to thedifference in the beginning of the 3rd exon and the end of the 5th exonof C-hsi13412 and C-kaia397. Therefore, the protein having the aminoacid sequence shown in SEQ ID NO: 1 and the protein having the aminoacid sequence shown in SEQ ID NO: 2 were found to be products ofalternately spliced forms derived from the same gene, respectively, withthe unique characteristic having different amino acid sequences fromeach other.

Example 5 Organs Expressing C-hsi13412 and C-kaia397

R30743, which is an EST identical to a part of the nucleotide sequenceregion encoding the amino acids of C-hsi13412, was isolated from fetalheart. This EST was found to be identical with 11 nucleotide sequencesthat seemed to be ESTs derived from the same gene by searching thenucleotide sequence of the 3′ end noncoding region of a cDNA cloneencoding C-hsi13412 in the nucleotide sequence databases GenBank, EMBL,and DDBJ using BLAST2. The GenBank accession numbers of these ESTs areAI658885, W28828, AI377545, AA896981, AA688321, AW370932, AA126609,AQ431640, AQ742986, AI971557, and AL030921. Among them, AI658885,W28828, AI377545, AA896981, AA688321, and AW370932 are registered asUniGene Hs.155234. AI658885 and AA688321 were isolated from prostategland, and W28828, AA896981, AW370932, and AA126609 were isolated fromeyes, spleen, mammary glands, and uterus of a pregnant woman,respectively.

Therefore, C-hsi13412 was presumed to be expressed in fetal heart,prostate gland, eye, spleen, mammary gland, and uterus of pregnantwomen. Similarly, the nucleotide sequence databases GenBank, EMBL, andDDBJ were searched for the nucleotide sequence of the 3′ end noncodingregion of a cDNA clone encoding C-kaia397 using BLAST2, and the sequencewas revealed to be identical to 7 nucleotide sequences that weresuggested to be ESTs derived from the same gene. The GenBank accessionnumbers of these ESTs are AA937577, AW080122, AA534966, AW374463,AQ394346, F23541, and F23538. AW080122, AA534966, and F23538 wereisolated from esophagus, large intestine, and muscles, respectively.Therefore, C-kaia397 was presumed to be expressed in esophagus, largeintestine, and muscles.

Example 6 Expression Analysis of C-hsi13412 and C-kaia397 Using RT-PCR

cDNA was synthesized using the commercially available SUPER SCRIPTPreamplification System for first strand cDNA Synthesis (GIBCO BRL)according to the attached instructions, and, as a template, 4 μg oftotal RNA that was prepared by the AGPC method (Analytical Biochemistry,162, 156 (1987); Experimental Medicine 9, 1937 (1991)) from 4 μg ofhuman organ polyA⁺ RNA purchased from Clontech and cancer cell line.

PolyA⁺ RNA derived from brain, kidney, pancreas, pituitary gland, smallintestine, bone marrow, heart, liver, lung, lymph node, mammary gland,placenta, prostate gland, skeletal muscle, spleen, stomach, testis,thymus, thyroid gland, and uterus were used as the human organ polyA⁺RNA.

T cell lines (Jurkat (Riken Cell Bank; RCB 086); Molt-3 (ATCCCRL-1552)), B cell lines (Namalwa KJM-1 (J. Biol. Chem., 268, 22782,1993); Daudi (ATCC CCL-213)), monocytic cell line (HL-60 (ATCCCCL-240)), vascular endothelial cell lines (HUVEC (Kurabo); IVEC (J.Cell. Physiol., 157, 41, 1993; N. T. L. FRANCE)), melanoma cell lines(WM266-4 (ATCC CRL-1676); WM115 (ATCC CRL-1675)), neuroblastoma celllines (SK− N-MC (ATCC HTB-10); SK-N-SH (ATCC HTB-11)), lung cancer celllines (PC-9 (Immuno-Biological Laboratories); HLC-1 (Osaka UniversityCancer Research Institute); QG90 (Aichi Cancer Center)), gastric cancercell line (KATO-III (Immuno-Biological Laboratories)), pancreatic cancercell lines (Capan-1 (ATCC HTB-79); Capan-2 (ATCC HTB-80)), colon cancercell lines (Colo 205 (ATCC CCL-222); SW1116 (Aichi Cancer Center); andLS180 (ATCC CCL-187)) were used as cancer cell lines.

Next, PCR was performed using the synthesized cDNA as a template.Specifically, using primers containing sequences specific to C-hsi13412and C-kaia397, and to human β-actin mentioned below, a reaction solutionwas prepared by a conventional method using a solution of thesynthesized cDNA that was diluted 50 times using sterilized water andAdvantage™ cDNA PCR kit (Clontech). Then, PCR was performed under areaction at 94° C. for 7 minutes, repeating 32 cycles, one cycleconsisting of reaction at 94° C. for 1 minute and reaction at 68° C. for3 minutes, and finally 68° C. for 7 minutes. This reaction solution wassubjected to agarose gel electrophoresis. By comparing the intensity ofthe DNA band specific to the used primers, the expression levels weresemi-quantitatively compared. As a result, the expression patterns ofC-hsi13412 and C-kaia397 were revealed to be different (FIG. 5).

The PCR was carried out using the oligonucleotides consisting of thenucleotide sequences shown in SEQ ID NO: 9 and SEQ ID NO: 10 as theprimer set specific for C-hsi13412, the oligonucleotides consisting ofthe nucleotide sequences shown in SEQ ID NO: 9 and SEQ ID NO: 11 as theprimer set specific for C-kaia397, and the oligonucleotides consistingof the nucleotide sequences shown in SEQ ID NO: 12 and SEQ ID NO: 13 asthe primer set specific for human β-actin. Primer-specific DNA bandswere confirmed in the above-mentioned RT-PCR reactions using theseprimer sets, and their sizes were approximately 730 bp, 310 bp, and 600bp for C-hsi13412, C-kaia397, and human β-actin, respectively.

Furthermore, regarding the difference in the expression levels betweennormal tissues and cancer tissues of human, RT-PCR was carried out underthe same conditions as mentioned above using Single Tumor Tissue MultiSample Breast 1 (BioChain) as a template. C-hsi13412 and C-kaia397 wereexpressed in both normal tissues and cancer tissues. However, a tendencyof decreased expression level was observed in the examined breast cancertissues (FIG. 6).

Example 7 Expression Analysis of C-hsi13412 and C-kaia397 Using NorthernBlotting Method

Expression patterns of C-hsi13412 and C-kaia397 among human cancertissues and normal tissues were examined as follows using 96 DotTumor/Normal Tissue Total RNA Dot Blot (BioChain).

(1) Construction of DNA Probes

Using either of the 2 clones produced in Example 2, C-hsi13412 orC-kaia397, as a template, a reaction solution was prepared by aconventional method using a primer set consisting of the nucleotidesequences shown in SEQ ID NO: 9 and SEQ ID NO: 14, and Ex Taq DNAPolymerase (TAKARA). Then, PCR was carried out under a reaction at 94°C. for 10 min; repeating 35 cycles, one cycle consisting of reaction at94° C. for 1 minute, reaction at 60° C. for 1 minute, and reaction at72° C. for 2 minutes; and finally at 72° C. for 10 minutes. Then,separating the amplification product of approximately 300 bp by 1%agarose gel electrophoresis to prepare a DNA probe common to C-hsi13412and C-kaia397.

The primer set consisting of the nucleotide sequences shown in SEQ IDNO: 9 and SEQ ID NO: 14 were designed based on the nucleotide sequencecommon between the coding region of C-hsi13412 and C-kaia397 set forthin SEQ ID NO: 3 and SEQ ID NO: 4, respectively.

(2) Constriction of Labeled DNA Probes

The DNA probe constructed in above (1) was labeled with a radioisotope(hereinafter, abbreviated as “RI”) by the random priming method (Anal.Biochem. 132, 6 (1983), Anal. Biochem. 137, 266 (1984)).

A reaction solution was prepared using 75 ng of the above-mentioned DNAprobe of (1), 5 μl of [α-³²P]dCTP (NEN), and Oligolabelling Kit(Amersham Pharmacia) according to the attached instructions, and thereaction was carried out at 37° C. for 1 hour. Then, the unreactedlabeled nucleotides were removed by centrifugation using Centri-Sep SpinColumn (PRONCETON SEPARATIONS) at 3,000 rpm for 2 minutes, to prepareRI-labeled DNA probe.

(3) Hybridization

Prehybridization was carried out by soaking the above-mentioned 96 DotTumor/Normal Tissue Total RNA Dot Blot membrane in 5 ml ofprehybridization solution (6×SSPE (6×(0.15 M NaCl, 8.65 mM NaH2PO4.2H2O,1.25 mM EDTA)), 2×Denhardt's Solution (Nacalai Tesque), 50% formamide,0.5% SDS, 100 μg Salmon Sperm DNA (Stratagene)), shaking at 42° C., andthen leaving standing for 3 hours. Meanwhile, the labeled DNA probeconstructed in above (2) was denatured into single strands by heating at94° C. for 5 minutes. After 3 hours of prehybridization, the membranewas soaked in 2 ml of fresh prehybridization solution, and the labeledDNA probe denatured into single strands were added so that the specificactivity of RI is 2×106 cpmrn/ml. Hybridization was carried out byleaving shaking at 42° C. for 20 hours.

(4) Membrane Washing

The membrane was recovered from the hybridization solution, and shakentwice in 2×SSPE containing 0.1% SDS at 42° C. for 10 minutes withexchange of the solvent and three times at 50° C. for 30 minutes to washthe membrane.

(5) Signal Detection

The washed membrane was placed into a cassette for X-ray films with anX-ray film, and autoradiography was performed by leaving standing at−80° C. for 24 hours to detect the signal.

As a result of the analysis of the signal, in normal cells, theexpression was mainly observed in stomach, small intestine, gallbladder, thyroid gland, kidney, bladder, thymus, prostate gland, andmammary gland. On the other hand, in cancer tissues, the expression wasmainly observed in stomach, pancreas, bladder, prostate gland, mammarygland, thyroid gland, and thymus. In addition, in kidney, gall bladder,small intestine, and thyroid gland, decrease of expression was observedin cancer tissues compared to normal tissues. However, significantdifferences were not exactly observed in other tissues. As shown inExample 6, since the expression patterns of C-hsi13412 and C-kaia397differ depending on the types of tissues and cancer, the importance ofexpression analysis by Northern Blotting using probes that do notdifferentiate between C-hsi13412 and C-kaia397, as well as expressionanalysis that differentiates between C-hsi13412 and C-kaia397 wassuggested.

Example 8 Expression of Proteins Encoded by C-hsi13412 or C-kaia397Using Animal Cells as hosts (1)

(1) Construction of Recombinant Vectors

(i) pcDNA3-C-hsi13412

Using the clone C-hsi13412 constructed in Example 2 as a template, andusing a primer set consisting of the nucleotide sequences shown in SEQID NO: 15 and SEQ ID NO: 16 and KOD DNA Polymerase (TOYOBO), a reactionsolution was prepared by a conventional method. Then PCR was performedunder a condition of 25 cycles, one cycle consisting of reaction at 98°C. for 15 seconds, reaction at 65° C. for 2 seconds, and reaction at 74°C. for 30 seconds. The primer set consisting of the nucleotide sequencesshown in SEQ ID NO: 15 and SEQ ID NO: 16 were designed so that FLAGmarker peptide is added to the C-terminus of C-hsi13412. Next,pSK-C-hsi13412 was constructed by inserting the obtained amplificationproduct of approximately 990 bp into the SmaI site of pBluescript II SK−(Stratagene), so that the 5′ side of C-hsi13412 is on the side of KpnIsite of pBluescript II SK−, and the 3′ side of C-hsi13412 on the side ofSacI site of pBluescript II SK−.

Next, the EcoRI-NotI fragment (900 bp) of pSK-C-hsi13412 was insertedinto the EcoRI-NotI site of pcDNA3 (Invitrogen), an expression vectorfor animal cells, to construct pcDNA3-C-hsi13412.

(ii) pcDNA3-C-kaia397

Using the clone C-kaia397 constructed in Example 2 as a template, andusing a primer set consisting of the nucleotide sequences shown in SEQID NO: 15 and SEQ ID NO: 17 and KOD DNA Polymerase (TOYOBO), a reactionsolution was prepared by a conventional method. Then PCR was performedunder a condition of 25 cycles, one cycle consisting of reaction at 98°C. for 15 seconds, reaction at 65° C. for 2 seconds, and reaction at 74°C. for 30 seconds. The primer set consisting of the nucleotide sequencesshown in SEQ ID NO: 15 and SEQ ID NO: 17 were designed so that FLAGmarker peptide is added to the C-terminus of C-kaia397. Next,pSK-C-kaia397 was produced by inserting the obtained amplificationproduct of approximately 620 bp into the SmaI site of pBluescript II SK−(Stratagene), so that the 5′ side of C-kaia397 is on the side of KpnIsite of pBluescript II SK−, and the 3′ side of C-kaia397 on the side ofSacI site of pBluescript II SK−.

Next, the EcoRI-NotI fragment (600 bp) of pSK-C-kaia397 was insertedinto the EcoRI-NotI site of pcDNA3 (Invitrogen), an expression vectorfor animal cells, to construct pcDNA3-C-kaia397.

(2) Insertion of Vectors into Cells

2 μl of FuGENE™ 6 transfection reagent (Roche Diagnostics) was added to100 μl of Opti-MEM (Gibco), and was left standing at room temperaturefor 5 minutes. Then, 1 μl of control plasmid pcDNA3, the C-hsi13412expression plasmid pcDNA-3-C-hsi13412 constructed in (1)(i) mentionedabove, or the C-kaia397 expression plasmid pcDNA3-C-kaia397 constructedin (1)(ii) mentioned above was added, and was left standing at roomtemperature for another 15 minutes. Meanwhile, 2 ml/well aliquots ofDMEM medium containing 10% dFCS were placed into a 6-well plate, andCOS-1 cells (Riken Cell Bank; RCB0143) were plated at 3×10⁵/well. Afterleaving standing for 15 minutes, 25 μl/well aliquots of a mixed solutionof the plasmid and FuGENE™ 6 transfection reagent were added to the6-well plate, and was cultivated at 37° C. for 72 hours in a CO₂incubator to obtain a transiently transfectant. Hereinafter, thetransfected cell lines produced by introducing pcDNA3, C-hsi13412, andC-kaia397 will be referred to as COS-1/mock cell line, COS-1/C-hsi13412cell line, and COS-1/C-kaia397 cell line, respectively.

(3) Confirmation of Expression

(i) Preparation of Culture Supernatants

Culture supernatants were obtained by collecting 4 ml of the culturesupernatants of COS-1/mock cell line, COS-1/C-hsi13412 cell line, andCOS-1/C-kaia397 cell line produced in above (2), and removing solids bycentrifugation at 1,200 rpm for 5 minutes. The obtained culturesupernatants were stored at −20° C., and were used in the followingexperiments upon thawing as necessary.

(ii) Acquisition of Cell Lysate

After detaching cells of COS-1/mock cell line, COS-1/C-hsi13412 cellline, and COS-1/C-kaia397 cell line produced in the above-mentioned (2)from the 6-well plate with a cell scraper (Sumitomo Bakelite), the cellswere suspended in 2 ml of Phosphate Buffered Saline (pH 7.2)(hereinafter, referred to as “PBS” (Gibco)), and were centrifuged at1,200 rpm for 5 minutes to remove the supernatant. Next, the resultingprecipitate was suspended in 1 ml of PBS containing 100 μl of proteaseinhibitor cocktail for animal cells (Sigma), and subjected to ultrasonichomogenization to obtain cell lysate. The obtained cell lysate wasstored at −20° C., and was used for the following experiments uponthawing as necessary.

(iii) Detection by Western Blotting

Western Blotting was carried out in 10 μl of the culture supernatantprepared in above (3)(i) or 5 μl of the cell lysate prepared in above(3)(ii) using anti-FLAG M2 monoclonal antibody (Sigma) as the detectionantibody according to Example 14.

Bands that were not observed in COS-1/mock cell line were detected inboth the cell lysate and culture supernatant of COS-1/C-hsi13412 cellline and COS-1/C-kaia397 cell line. Therefore, the expression ofproteins encoded by C-hsi13412 and C-kaia397 were confirmed in COS-1cells (FIG. 7).

Example 9 Expression of Proteins Encoded by C-hsi13412 or C-kaia397Using Animal Cells as Hosts (2)

(1) Construction of Recombinant Vectors

(i) Construction of pAGE210-C-hsi13412 and pAGE210-C-hsi13412h

Using pSK-C-hsi13412 produced in Example 8 as a template, anoligonucleotide primer set consisting of the nucleotide sequences shownin SEQ ID NO: 18 and SEQ ID NO: 19, or an oligonucleotide primer setconsisting of the nucleotide sequences shown in SEQ ID NO: 18 and SEQ IDNO: 20, and native pfu polymerase (STRATAGENE), a reaction solution wasprepared by a conventional method. Then PCR was performed under areaction condition of 94° C. for 5 minutes; 25 repeating cycles, onecycle consisting of reaction at 94° C. for 1 minute, reaction at 55° C.for 1 minute, and reaction at 72° C. for 2 minutes; and finally 72° C.for 7 minutes.

Next, pSK-C-hsi13412c and pSK-C-hsi13412h were constructed by insertingthe obtained amplification product of approximately 900 bp into theHindIII-XbaI site of pBluescript II SK−.

Then, the HindIII-XbaI fragments (900 bp) of pSK-C-hsi13412c andpSK-C-hsi13412h were inserted into the HindIII-XbaI site of pAGE210, avector for animal cell expression (J. Biochem., 101, 1307 (1987)), toconstruct pAGE210-C-hsi13412 and pAGE210-C-hsi13412h.

(ii) pAGE210-C-kaia397 and pUC-C-kaia397h

Using the pSK-C-kaia397 produced in Example 8 as a template, and anoligonucleotide primer set consisting of the nucleotide sequences shownin SEQ ID NO: 18 and SEQ ID NO: 21 or an oligonucleotide primer setconsisting of the nucleotide sequences shown in SEQ ID NO: 18 and SEQ IDNO: 22, PCR was performed as described in above (i).

Next, the obtained amplification product of approximately 600 bp wasinserted into the HindIII-XbaI site of pBluescript II SK− to constructpSK-C-kaia397c and pSK-C-kaia397h.

Then, the HindIII-XbaI fragments (600 bp) of pSK-C-kaia397c andpSK-C-kaia397h were inserted into the HindIII-XbaI sites of pAGE210 andpUC18 (Amersham Pharmacia) to construct pAGE210-C-kaia397 andpUC-C-kaia397h.

(2) Introduction of Vectors into Cells

pAGE210-C-hsi13412, pAGE210-C-hsi13412h, pAGE210-C-kaia397, andpUC-C-kaia397h constructed in above (1)(i) and (ii) were introduced intoCHO cells (Somatic Cell and Molecular Genetics, 12, 555 (1986))) by theelectroporation method (Cytotechnology, 3, 133 (1990))) as follows.

pAGE210, pAGE210-C-hsi13412, pAGE210-C-hsi13412h, pAGE210-C-kaia397, andpUC-C-kaia397h were cleaved at FspI, and were linearized. Afterphenol-chloroform extraction treatment, ethanol precipitation wascarried out, and the obtained linearized plasmids were dissolved in TEsolution. On the other hand, CHO cells were subcultured in αMEM1900medium (Gibco) supplemented with 5% fetal calf serum (Gibco), 0.09%sodium bicarbonate (Gibco), and 1% Penicillin-streptomycin (Gibco)(hereinafter, referred to as “A medium”) and were used for theexperiment. The CHO cells were suspended in K-PBS buffer (137 nmol/lpotassium chloride, 2.7 nmol/l sodium chloride, 8.1 mmol/l disodiumhydrogen phosphate, 1.5 nmol/l sodium dihydrogen phosphate, 4 mmol/1magnesium chloride buffer) to a concentration of 8×10⁶ cells/ml, and 200μl of the cell suspension solution (containing 1.6×10⁶ cells) were mixedwith 4 μg of the above-mentioned linearized plasmid. The mixed solutionwas transferred to a cuvette (inter-electrode distance of 2 mm), andusing Gene Pulser II (BioRad) apparatus, gene transfer was carried outunder a condition of pulse voltage of 0.30 kV, and capacitance of 250μF. After leaving the cuvette standing on ice, the cell suspension inthe cuvette was suspended in 15 ml of A medium, a 5 ml aliquot thereofwas placed into a flask and was cultivated in a 5% carbon dioxide gasincubator at 37° C. After cultivating for 1 day, the medium wasexchanged to αMEM2000 medium supplemented with 5% fetal calf serum,0.09% sodium bicarbonate, 1% Penicillin-streptomycin, and 300 μg/mlhygromycin B (Gibco) (hereinafter, referred to as “B medium”), andcultivation was continued. The subculturing was continued with dilutionduring the process, and approximately 2 weeks after gene transfer,transfected cell line having resistance to hygromycin B was obtained.The transfected cell line was subcultured in B medium. Hereinafter,transfected cell lines produced by introducing pAGE210,pAGE210-C-hsi13412, pAGE210-C-hsi13412h, pAGE210-C-kaia397, andpUC-C-kaia397h will be referred to as CHO/mock cell line, CHO/C-hsi13412cell line, CHO/C-hsi13412h cell line, CHO/C-kaia397 cell line, andCHO/C-kaia397h cell line, respectively.

(3) Ascertainment of Expression

CHO/mock cell line, CHO/C-hsi13412 cell line, CHO/C-hsi13412h cell line,CHO/C-kaia397 cell line, and CHO/C-kaia397h cell line produced in above(2) were cultivated by static culture using B medium. Cells plated at acell density of approximately one fifth confluence were grown to a statewherein the cells almost completely filled the surface of a 25cm²-cultivation container, and the culture supernatant and the cellswere collected separately. 100 μl of the collected culture supernatantwas subjected to organic solvent precipitation using acetone, and thepellet was resolved in 50 μl of 100 mmol/l Tris-HCl buffer (pH 7.5) toprepare a supernatant fraction. Cells were collected using a cellscraper, suspended into 500 μl of 100 mmol/l Tris-HCl buffer (pH 7.5),and then subjected to ultrasonic homogenization to prepare a cellfraction. After separating 11.25 μl each of the prepared supernatantfraction or the cell fraction by SDS polyacrylamide gel electrophoresisaccording to the Laemmli method, Western Blotting analysis was carriedout according to Example 14 using anti-C-hsi13412/C-kaia397 monoclonalantibody KM2962 produced in Example 13.

In all of the CHO/C-hsi13412, CHO/C-hsi13412h, CHO/C-kaia397, andCHO/C-kaia397h cell lines, expression of C-hsi13412 or C-kaia397 wasascertained in both the culture supernatant and cell fractions. Theexpression levels were higher in the cell fractions than in the culturesupernatants in all of the transfected cell lines. Furthermore,regarding the molecular weight, when COS-1/C-hsi13412 cell line andCOS-1/C-kaia397 cell line were expressed using COS-1 cell as the host,nearly identical mobilities were indicated.

As mentioned above, the expression of C-hsi13412 and C-kaia397 in CHOcells was ascertained (FIG. 8).

(4) Ascertainment of Glycosylation

Next, 7.5 μl of 1% SDS was added to 36 μl of the supernatant fraction or36 μl of the cell fraction prepared in above (3), and was denatured byheating at 95° C. for 5 minutes. After the denaturing, 5 μl of 5%Nonidet P-40 (NP-40; polyoxyethylenenonylphenyl ether (Sigma)), 2 μl of25 mM/50 μl of Glycopeptidase F (N-type glycosidase (Takara)), and 1 μlof 25 mM/50 μl of o-Glycosidase (o-type glycosidase (Roche Diagnostics))were added to 14.5 μl of the supernatant fraction or 14.5 μl of the cellfraction, and was reacted at 37° C. overnight. As a control, a reactionsolution in which 8 μl of 100 mmol/l Tris-HCl buffer (pH 7.5) was addedinstead of a glycosidase solution was prepared. Using 11.25 μl of thesupernatant fraction or 11.25 μl of the cell fraction after reaction,Western Blotting analysis was performed as in above (3).

Since the molecular weights changed due to the addition of glycosidases,proteins encoded by C-hsi13412 and C-kaia397 produced by the transfectedcells were presumed to be glycosylated (FIG. 8).

Example 10 Expression of Protein Encoded by C-hsi13412 Using InsectCells as the Host

To produce recombinant proteins in insect cells, a recombinant viruswherein the desired gene has been incorporated has to be constructed.Such construction includes the steps of: (1) constructing a specificvector carrying a cDNA encoding the desired protein; (2) cotransfectinga baculovirus DNA and a transfer vector into insect cells to construct arecombinant virus by homologous recombination, and then growing them;and (3) expressing the desired protein by infecting cells with therecombinant virus. The details are described below.

(1) Construction of a Transfer Vector

pVL-C-hsi13412, a transfer vector encoding the full length of C-hsi13412(the amino acid sequence of the amino acid residues at position 1 to 304of SEQ ID NO: 1), was constructed by inserting the EcoRI-BamHI fragment(900 bp) of plasmid pSK-C-hsi13412 of Example 8 into the EcoRI-BamHIsite of insect cell transfer vector pVL1392 (PharMingen).

(2) Construction of a Recombinant Virus

Recombinant baculovirus was constructed by introducing linearbaculovirus DNA (BaculoGold Baculovirus DNA (PharMingen)) and thetransfer vector constructed in above (1) using the lipofectin method(Protein, Nucleic Acid, and Protein, 37, 2701 (1992)) to insect cellsSf9 (Iwaki Glass) cultivated in ESF921 medium (Protein Expression). Thedetails are described below.

pVL-C-hsi13412 and 15 ng of linear baculovirus DNA were dissolved in 12μl of sterilized distilled water, a mixture of 6 μl of lipofectin and 6μl of sterilized distilled water was added thereto, and the mixture wasleft standing at room temperature for 15 minutes. Meanwhile, 1×10⁶ Sf9cells were suspended in 2 ml of ESF921 medium, and was placed into a 50mm diameter plastic Petri dish for cell cultivation. All of the mixedsolution of the above-mentioned plasmid DNA, linear baculovirus DNA, andlipofectin were added to the dish, and after cultivation at 27° C. for 3days, 1 ml of the culture supernatant containing the recombinant viruswas collected. 1 ml of ESF921 medium was freshly added to the Petridish, and the dish was cultivated at 27° C. for additional 3 days toobtain another 1.5 ml of culture supernatant containing the recombinantvirus.

Next, a recombinant virus that retains the C-hsi13412 gene was grown bythe following procedure.

Sf9 cells were inoculated into 50 ml ESF921 medium at 5×10⁵/ml, and werecultivated with shaking at 125 rpm at 27° C. using a 125 ml Erlenmeyerflask. When the cells grew to 2×10⁶/ml, the cells were infected with therecombinant virus at MOI=10, and were further cultivated for 3 days. Theculture solution was centrifuged at 1,200 rpm for 10 minutes to removethe cells, and a recombinant virus solution for protein expression wasobtained.

The titer of the recombinant virus solution was measured by thefollowing method.

6×10⁵ Sf9 cells were suspended in 4 ml of ES F921 medium, placed into a50 mm diameter plastic Petri dish for cell culture, and adhered to thePetri dish by leaving standing at room temperature for 1 hour. Thesupernatant was removed, 100 μl of the above-mentioned recombinant virussolution diluted with ESF921 medium and 400 μl of ESF921 medium wereadded, after leaving standing at room temperature for 1 hour, the mediumwas removed, and 5 ml of a medium containing 1% low-melting-pointagarose (AgarPlaque Agarose (PharMingen)) (a medium prepared by mixing 1ml of sterilized 5% aqueous AgarPlaque Agarose solution and 4 ml ofTMN-FH Insect Medium (PharMingen), and warming at 42° C.) was pouredinto the Petri dish. After leaving standing at room temperature for 15minutes, the Petri dish was sealed with a vinyl tape to prevent drying,was placed into a sealable plastic container, and was cultivated at 27°C. for 5 days. 1 ml of PBS containing 0.01% neutral red was added to thePetri dish, and was cultivated for another day. Then, the number ofplaques that appeared was counted, and the preparation of a 0.5 to2×10⁸/ml recombinant virus solution was confirmed.

(3) Expression of Proteins

Sf9 cells were placed into 100 ml of ESF921 medium at 5×10⁵/ml, and werecultured while shaking at 125 rpm at 27° C. using a 250 ml Erlenmeyerflask. When the cells grew to 3 to 4×10⁶/ml, the cells were subculturedinto a flask with a base area of 182 cm² containing 25 ml of ESF921media, so as to contain 3×10⁷ cells. After adhering the cells by leavingstanding at room temperature for 1 hour, the medium was removed, and therecombinant virus carrying the C-hsi13412 gene was added at MOI=5. ThenESF921 medium was further added to a total volume of 10 ml, and wasinfected at room temperature for 1 hour. Subsequently, 20 ml of ESF921medium was added, and the desired recombinant protein was expressed byculture at 27° C. for 3 days.

Western Blotting using anti-FLAG M2 monoclonal antibody (Sigma) wascarried out according to Example 14, using 5 μl of cell suspension ofcells that were infected with the recombinant virus carrying theC-hsi13412 gene and 20 μl of the culture supernatant as samples.

A band at a molecular weight of approximately 33 kDa, which was notobserved in uninfected cells, was detected in both the cell suspensionand the culture supernatant of cells that were infected with C-hsi13412(FIG. 9).

Example 11 Preparation of Antigens for Producing Antibodies AgainstProteins Encoded by C-hsi13412 or C-kaia397

The amino acid sequences of the proteins encoded by C-hsi13412 andC-kaia397 were analyzed, and compound 1 (a polypeptide having the aminoacid sequence shown in SEQ ID NO: 23), compound 2 (a polypeptide havingthe amino acid sequence shown in SEQ ID NO: 24), and compound 3 (apolypeptide having the amino acid sequence shown in SEQ ID NO: 25) wereselected from highly hydrophilic regions, N-termini, C-termini, regionshaving a turn structure in their secondary structure, and a regionhaving a random coil structure, as partial sequences that were suggestedto be suitable as antigens.

The amino acids used in the present invention and the abbreviations ofthe protecting groups thereof were in accordance with therecommendations of IUPAC-IUB Joint Commission on BiochemicalNomenclature (European Journal of Biochemistry, 138, 9 (1984)).

The following abbreviations represent the corresponding amino acidsmentioned below, unless stated otherwise.

Ala: L-alanine

Asn: L-asparagine

Asp: L-aspartic acid

Asx: L-aspartic acid or L-asparagine

Arg: L-arginine

Cys: L-cysteine

Glu: L-glutamic acid

Glx: L-glutamic acid or L-glutamine

Gly: glycine

Leu: L-leucine

Phe: L-phenylalanine

Pro: L-proline

Ser: L-serine

Thr: L-threonine

Trp: L-tryptophan

Tyr: L-tyrosine

Met: L-methionine

Val: L-valine

The following abbreviations represent the corresponding amino acidprotecting groups and side chain protected amino acids mentioned below.

Fmoc: 9-fluorenylmethyloxycarbonyl

tBu: t-butyl

Trt: trityl

Boc: t-butoxycarbonyl

Pmc: 2,2,5,7,8-pentamethylchroman-6-sulfonyl

Fmoc-Arg(Pmc)-OH:

N^(α)-9-fluorenylmethyloxycarbonyl-N^(γ)-7-2,2,5,7,8-pentamethylchroman-6-sulfonyl-L-arginine

Fmoc-Asn(Trt)-OH:N^(α)-9-fluorenylmethyloxycarbonyl-N^(γ)-trityl-L-asparagine

Fmoc-Asp(OtBu)-OH: N^(α)-9-fluorenylmethyloxycarbonyl-L-asparticacid-β-t-butylester

Fmoc-Cys(Trt)-OH: N^(α-)9-fluorenylmethyloxycarbonyl-5-trityl-L-cysteine

Fmoc-Glu(OtBu)-OH: N^(α)-9-fluorenylmethyloxycarbonyl-L-glutamicacid-γ-t-butylester

Fmoc-Ser(tBu)-OH: N^(α)-9-fluorenylmethyloxycarbonyl-O-t-butyl-L-serine

Fmoc-Thr(tBu)-OH:N^(α)-9-fluorenylmethyloxycarbonyl-O-t-butyl-L-threonine

Fmoc-Trp(Boc)-OH:N^(α)-9-fluorenylmethyloxycarbonyl-N^(ind)-t-butyloxycarbonyl-L-tryptophan

Fmoc-Tyr(tBu)-OH:N^(α)-9-fluorenylmethyloxycarbonyl-O-t-butyl-L-tyrosine

The following abbreviations represent the corresponding reactionsolvents, reaction reagents, and such mentioned below.

HBTU: 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluroniumhexafluorophosphate

HOBt: N-hydroxybenzotriazole

DIEA: diisopropylethylamine

DMF: N,N-dimethylformamide

TFA: trifluoroacetic acid

The physicochemical properties of the compounds were measured by thefollowing method in Examples below.

Mass spectrometry was carried out by the FAB-MS method using JEOLJMS-HX110A, or by the MALDI-TOFMS method using Brucker Mass SpectrometerREFLEX. Amino acid analysis was carried out by the method of Cohen, S.A. et al (Analytical Biochemistry, 222, 19 (1994)). Hydrolysis wascarried out in hydrochloric acid vapor at 110° C. for 20 hours, and theamino acid composition of the hydrolysis product was analyzed usingWaters AccQ-Tag amino acid analyzer (Waters).

(1) Synthesis of Compound 1 (a Peptide Comprising the Amino AcidSequence Shown in SEQ ID NO:23,H-Cys-Arg-Pro-Ser-Pro-Gly-Pro-Asp-Tyr-Leu-Arg-Arg-Gly-Trp-Met-Arg-Leu-NH₂)

30 mg of carrier resin (Rink amide MBHA resin, Novabiochem) bound with18 μmol of Fmoc-NH were placed into a reaction container of automaticsynthesizer (Shimadzu). 600 μl of DMF was added thereto, and was stirredfor 3 minutes, and the solution was drained. Then, the followingprocedures were carried out according to a synthesis program ofShimadzu.

(a) 500 μl of 30% piperidine-DMF solution was added, the mixture wasstirred for 4 minutes, the solution was drained, and this operation wasrepeated once again.

(b) The carrier resin was washed with 600 μl of DMF for 1 minute, thesolution was drained, and this operation was repeated 5 times.

(c) Fmoc-Leu-OH (165 μmol), HBTU (165 μmol), HOBt monohydrate (165μmol), and DIEA (330 μmol) were stirred in DMF (660 μl) for 3 minutes,the obtained solution was added to the resin, the mixture was stirredfor 60 minutes, and the solution was drained.

(d) The carrier resin was washed with 900 μl of DMF for 1 minute, thesolution was drained, and this operation was repeated 5 times.

Fmoc-Leu-NH was synthesized on the carrier by the above-mentioned steps.

Next, Fmoc-Arg(Pmc)-Leu-NH was synthesized on the carrier through thesteps (a) and (b), a condensation reaction using Fmoc-Arg(Pmc)-OH instep (c), and the washing step of (d).

A carrier resin bound with side-chain-protected peptides were obtainedby repeating the steps (a) through (d) using Fmoc-Met-OH,Fmoc-Trp(Boc)-OH, Fmoc-Gly-OH, Fmoc-Arg(Pmc)-OH, Fmoc-Arg(Pmc)-OH,Fmoc-Leu-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Pro-OH,Fmoc-Gly-OH, Fmoc-Pro-OH, Fmoc-Ser(tBu)-OH, Fmoc-Pro-OH,Fmoc-Arg(Pmc)-OH, and Fmoc-Cys(Trt)-OH in order in the step (c);followed by the deprotection and washing steps of (a) and (b), washingwith methanol and butyl ether in order, and then drying under reducedpressure for 12 hours. 1 ml of a mixed solution comprising TFAcontaining 2-methylindole at a concentration of 5 mg/mL (82.5%),thioanisole (5%), water (5%), ethyl methyl sulfide (3%),1,2-ethanedithiol (2.5%), and thiophenol (2%) was added thereto, and wasleft standing at room temperature for 6 hours. Then the peptides werecleaved from the resin together with the removal of the side-chainprotecting groups. After filtering off the resin, approximately 10 ml ofether were added to the obtained solution, the generated precipitateswere collected by centrifugation and decantation, and 42.1 mg of crudepeptide was obtained. This crude product was dissolved in aqueous aceticacid solution, and then passed through a cartridge filled withreverse-phase silica gel (YMC Dispo SPE C18) to adhere the peptides.Upon washing with an aqueous solution of 0.1% TFA and 15% acetonitrileand eluting with an aqueous solution of 0.1% TFA and 25% acetonitrile, afraction containing compound 1 was obtained. This was freeze-dried, and28.9 mg of compound 1 was obtained.

Mass spectrometry [FABMS]; m/z=2058.9 (M+H⁺)

Amino acid analysis; Asx 1.0 (1), Ser 1.0 (1), Gly 2.0 (2), Arg 3.9 (4),Pro 2.9 (3), Tyr 1.0 (1), Met 1.0 (1), Leu 2.1 (2), Cys 1.2 (1)

(2) Synthesis of Compound 2 (a Peptide Comprising the Amino AcidSequence Shown in SEQ ID NO: 24,H-Cys-Arg-Pro-Pro-Ala-Phe-Thr-Pro-Arg-Ala-Pro-Asp-Arg-Val-Thr-Ser-Ile-OH)

Using 30 mg of carrier resin (Wang resin, Novabiochem) bound with 14.4μmol of Fmoc-Ile as the starting material, similarly to (1),Fmoc-Ser(tBu)-OH, Fmoc-Thr(tBu)-OH, Fmoc-Val-OH, Fmoc-Arg(Pmc)-OH,Fmoc-Asp(OtBu)-OH, Fmoc-Pro-OH, Fmoc-Ala-OH, Fmoc-Arg(Pmc)-OH,Fmoc-Pro-OH, Fmoc-Thr(tBu)-OH, Fmoc-Phe-OH, Fmoc-Ala-OH, Fmoc-Pro-OH,Fmoc-Pro-OH, Fmoc-Arg(Pmc)-OH, and Fmoc-Cys(Trt)-OH were condensed inorder. Then upon removing Fmoc, washing, and drying, carrier resin boundwith side-chain-protected peptides were obtained. 1 ml of a mixedsolution comprising TFA (82.5%), thioanisole (5%), water (5%), ethylmethyl sulfide (3%), 1,2-ethanedithiol (2.5%), and thiophenol (2%) wasadded thereto, and was left standing at room temperature for 8 hours.Then the peptides were cleaved from the resin together with the removalof the side-chain protecting groups. After filtering off the resin,approximately 10 ml of ether were added to the obtained solution. Thegenerated precipitates were collected by centrifugation and decantation,and 29.2 mg of crude peptide was obtained. This crude product wasdissolved in aqueous acetic acid solution, and then passed through acartridge filled with reverse-phase silica gel (YMC Dispo SPE C18) toadhere the peptides. Upon washing with an aqueous solution of 0.1% TFAand 10% acetonitrile, and eluting with an aqueous solution of 0.1% TFAand 25% acetonitrile, a fraction containing compound 2 was obtained.This was freeze-dried, and 24.5 mg of compound 2 was obtained.

Mass spectrometry [TOFMS]; m/z=1883.6 (M+H⁺)

Amino acid analysis; Asx 1.0 (1), Ser 1.0 (1), Arg 3.0 (3), Thr 1.9 (2),Ala 2.1 (2), Pro 4.0 (4), Val 1.0 (1), Ile 1.0 (1), Phe 1.0 (1), Cys 1.3(1)

(3) Synthesis of Compound 3 (a Peptide Comprising the Amino AcidSequence Shown in SEQ ID NO: 25,H-Cys-Asn-Leu-Val-Pro-Glu-Glu-Glu-Ala-Glu-Ser-Glu-Glu-Asn-Asp-Asp-Tyr-Tyr-OH)

Using 30 mg of carrier resin (SynProPep Resin, Shimadzu) bound with 18μmol of Fmoc-Tyr(tBu) as the starting material, similarly to (1),Fmoc-Asp(OtBu)-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Asn(Trt)-OH,Fmoc-Glu(OtBu)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Ser(tBu)-OH,Fmoc-Glu(OtBu)-OH, Fmoc-Ala-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Glu(OtBu)-OH,Fmoc-Glu(OtBu)-OH, Fmoc-Pro-OH, Fmoc-Val-OH, Fmoc-Leu-OH,Fmoc-Asn(Trt)-OH, and Fmoc-Cys(Trt)-OH were condensed in order. Uponremoving Fmoc, washing, and drying, the carrier resin bound withside-chain-protected peptides were obtained. 1 ml of a mixed solutioncomprising TFA (90%), thioanisole (5%), and 1,2-ethanedithiol (5%), wasadded thereto, and was left standing at room temperature for 2 hours.Then the peptides were cleaved from the resin together with the removalof the side-chain protecting groups. After filtering off the resin,approximately 10 ml of ether were added to the obtained solution. Thegenerated precipitates were collected by centrifugation and decantation,and 32.5 mg of crude peptide was obtained. This crude product wasdissolved in a mixed solution comprising acetic acid, dithiothreitol,DMF, and water, and then passed through a cartridge filled withreverse-phase silica gel (YMC Dispo SPE C18) to adhere the peptides.Upon washing with an aqueous solution of 0.1% TFA and 10% acetonitrile,and eluting with an aqueous solution of 0.1% TFA and 25% acetonitrile, afraction containing compound 3 was obtained. This was freeze-dried, and17.1 mg of compound 3 was obtained.

Mass spectrometry [TOFMS]; m/z=2148 (M+H⁺)

Amino acid analysis; Asx 4.0 (4), Glx 6.2 (6), Ser 1.0 (1), Ala 1.1 (1),Pro 1.0 (1), Tyr 1.8 (2), Val 0.9 (1), Leu 0.9 (1), Cys 1.0 (1)

Example 12 Production of Polyclonal Antibodies that Recognize ProteinsEncoded by C-hsi13412 or C-kaia397

(1) Preparation of Immunogens

Conjugates of compound 2 and compound 3 obtained in Example 11 with KLH(Calbiochem) was prepared by the following method to raiseimmunogenicity, and were used as immunogens. More specifically, a 10mg/ml solution was prepared by dissolving KLH in PBS, and 1/10 thevolume of 25 mg/ml MBS (N-(m-Maleimidobenzoyloxy)succinimide: NacalaiTesque) was added drop wise, and this was allowed to react whilestirring for 30 minutes. Next, 2.5 mg of KLH-MB, which was obtained byremoving free MBS using Sephadex G-25 column (Amersham Pharmacia)pre-equilibrated in PBS, was mixed with 1 mg of the peptide dissolved in0.1 M sodium phosphate buffer (pH 7.0), and were allowed to react whilestirring for 3 hours at room temperature. After the reaction, theproduct, which was dialyzed with PBS, was used as the immunogen.

(2) Immunization of Animals and Preparation of Antisera

100 μg each of KLH conjugates of compound 2 and compound 3 prepared inabove (1) was administered to a 6 to 8 week old female BALB/c mouse,along with 2 mg of Aluminum hydroxide adjuvant (Antibodies-A LaboratoryManual, Cold Spring Harbor Laboratory, p99, 1988) and 1×10⁹ cells ofwhooping-cough vaccine (Chiba Prefecture Serum Research Institute). Twoweeks after the administration, 100 μg of each KLH conjugates wasadministered once a week, in total 4 times. Blood samples were collectedfrom the carotid arteries of the mice, and antisera were prepared.

(3) Western Blotting Using Immunized Mouse Antisera

Using 5 μl each of cell lysates of COS-1/C-hsi13412 cell line andCOS-1/C-kaia397 cell line obtained in Example 8 as samples of proteinsencoded by C-hsi13412 and C-kaia397, Western Blotting was carried outaccording to Example 14, using 500 μl of the immunized mouse antiseraobtained in above (2) as the detection antibody.

The mouse antisera that were obtained using compound 2 and compound 3 asimmunogens were confirmed to be able to specifically recognize proteinsencoded by C-hsi13412 and C-kaia397, respectively (FIG. 10).

Example 13 Monoclonal Antibodies Recognizing Proteins Encoded byC-hsi13412 or C-kaia397

(1) Preparation of Immunogens

A conjugate of compound 1 obtained in Example 11 with KLH was preparedaccording to a method similar to Example 12(1), and was used as theimmunogen.

(2) Immunization of Animals and Preparation of Antibody-Producing Cells

100 μg each of the KLH conjugate of compound 1 prepared in above (1) wasadministered to each of the three 6 to 8 week old female BALB/c mice,along with 2 mg of Aluminum hydroxide adjuvant (Antibodies-A LaboratoryManual, Cold Spring Harbor Laboratory, p 99, 1988) and 1×10⁹ cells ofwhooping-cough vaccine (Chiba Prefecture Serum Research Institute). Twoweeks after the administration, 100 μg each of the KLH conjugates wereadministered once a week, in total 4 times. Blood samples were collectedfrom the carotid arteries of the mice, their blood serum antibody titerswere examined by enzyme immunoassay method shown below, and the spleenwas excised from mice indicating sufficient antibody titers 3 days afterfinal immunization.

The excised spleen was fragmented in MEM (Minimum Essential Medium,NISSUI PHARMACEUTICAL), loosened with tweezers, and then centrifuged(250×g, 5 minutes). Tris-Ammonium chloride buffer (pH 7.6) was added tothe obtained precipitate fraction, and upon treatment for 1 to 2minutes, red blood cells were removed. The obtained precipitate fraction(cell fraction) was washed 3 times in MEM, and was used for cell fusion.

(3) Enzyme Immunoassay (Binding ELISA)

Compound 1 obtained in Example 11 that was conjugated with thyroglobulin(hereinafter, referred to as “THY”) was used as an antigen for assays.The production method was carried out similarly to Example 12, exceptusing SMCC (4-(N-Maleimidomethyl)-cyclohexane-1-carboxylic acidN-hydroxysuccinimido ester; Sigma) instead of MBS as the crosslinkingagent. For adhesion, 50 μl/well aliquots of the conjugate (10 μg/ml)prepared as mentioned above were dispensed into a 96-well EIA plate(Greiner), and were left standing overnight at 4° C. After washing theplate, the remaining active groups were blocked by adding 100 μl/wellaliquots of 1% bovine serum albumin (hereinafter, referred to as“BSA”)/Dulbecco's phosphate buffer (Phosphate buffered saline: PBS), andleaving standing at room temperature for 1 hour.

After leaving standing for 1 hour, 1% BSA/PBS was discarded, 50 μl/wellaliquots of immunized mouse antisera, culture supernatant of monoclonalantibodies, or purified monoclonal antibodies were dispensed into theplate, and were left standing for another 2 hours. This plate was washedwith 0.05% polyoxyethylene (20) sorbitan monolaurate (product name: Span20 (corresponding ICI trademarked product Tween 20: Wako))/PBS(hereinafter, abbreviated as “Tween-PBS”). Then ABTS substrate solution(2,2-azinobis(3-ethylbenzothiazol-6-sulfonic acid) ammonium, 1 mMABTS/0.1 M citric acid buffer (pH 4.2)) was added to develop colors, andthe absorbance at OD415 nm was measured using plate reader (Emax;Molecular Devices).

(4) Preparation of Mouse Myeloma Cells

By culturing 8-Azaguanine-resistant mouse myeloma cell line P3X63Ag8U.1(P3-U1: purchased from ATCC) in normal medium (RPMI medium containing10% fetal calf serum (hereinafter, abbreviated as “FCS”)) 2×10⁷ or morecells were obtained, and were used as a parent cell line for cellfusion.

(5) Production of Hybridomas

Mouse splenocytes obtained in above (2) and the myeloma cells obtainedin above (4) were mixed at a ratio of 10:1, and the mixture wascentrifuged (250× g, 5 minutes). The cell population of the obtainedprecipitate fraction was loosened well; then, while stirring, 0.5 ml ofa mixed solution containing 2 g of polyethylene glycol-1000 (PEG-1000),2 ml of MEM, and 0.7 ml of dimethyl sulfoxide was added at 37° C. forevery 108 mouse splenocytes. After 1 ml each of MEM was added to thesuspension several times every 1 to 2 minutes, MEM was added so that thetotal volume becomes 50 ml.

The suspension was centrifuged (900 rpm, 5 minutes), and after gentlyloosening the cells of the obtained precipitate fraction, the cells weresuspended into 100 ml of HAT media (a 10% FCS-added RPMI mediasupplemented with HAT/Media Supplement (Roche Diagnostics)) by gentlepipetting with a graduated pipette. 200 μl/well aliquots of thesuspension were dispensed into the 96-well culture plate, and werecultured for 10 to 14 days at 37° C. in a 5% CO₂ incubator.

After cultivation, the culture supernatant was examined by the enzymeimmunoassay method of (3) mentioned above, and wells that react to theantigenic peptide but not to the control peptide were selected. Usingcells included in the selected wells, cloning by the limiting dilutionmethod was repeated twice, and anti-C-hsi13412/C-kaia397 monoclonalantibody-producing hybridoma was established. As a result, usingcompound 1 as the antigen, two types of anti-C-hsi13412/C-kaia397monoclonal antibodies, KM2961 and KM2962, were obtained. KM2961 andKM2962 specifically reacted with compound 1, which is the antigenicpeptide (FIG. 14).

Hybridoma cell lines that produce anti-C-hsi13412/C-kaia397 monoclonalantibodies, KM2961 and KM2962, have been deposited under the accessionnumbers FERM BP-7603 and FERM BP-7604, respectively, in the NationalInstitute of Advanced Industrial Science and Technology, PatentMicroorganism Depository (AIST Tsukuba Central 6, 1-1-1 Higashi,Tsukuba, Ibaraki, Japan: postal code 305-8366) on May 24, 2001.

(6) Purification of Monoclonal Antibodies

The hybridoma cell lines obtained in (5) mentioned above were injectedintraperitoneally at 5 to 20×10⁶ cells/mouse to each pristane-treated8-week nude female mice (BALC/c). 10 to 21 days after the injection,ascites (1 to 8 ml/mouse) were collected from mice wherein ascites hadaccumulated due to the formation of ascites carcinoma from thehybridomas.

The ascites were centrifuged (1200×g, 5 minutes), and the solids wereremoved. The purified IgG monoclonal antibodies were obtained bypurification using the caprylic acid precipitation method (Antibodies—ALaboratory Manual, Cold Spring Harbor Laboratory, 1988). The subclassesof the monoclonal antibodies were determined to be IgG1 for KM2961 andIgG3 for KM2962 by the ELISA method using subclass typing kit.

Example 14 Detection of Proteins Encoded by C-hsi13412 or C-kaia397 byWestern Blotting Using KM2961 and KM2962

2.5 μl/lane of the cell lysates and 10 μl/lane of the culturesupernatant of COS-1/mock cell line, COS-1/C-hsi13412 cell line, andCOS-1/C-kaia397 cell line prepared in Example 8 were fractionated by 15%SDS-polyacrylamide gel electrophoresis (Antibodies—A Laboratory Manual,Cold Spring Harbor Laboratory, 1988), and then was blotted onto a PVDFmembrane (Millipore) according to a conventional method. After blockingthe membrane with 5% skim milk-PBS (hereinafter, referred to as“blocking solution”), anti-C-hsi3412/C-kaia397 monoclonal antibody,KM2961 or KM2962 was added to the membrane at a concentration of 2μg/ml, and was left standing at room temperature for 1 hour. Afterwashing the membrane well with Tween-TBS, detection was carried outusing peroxidase-labeled rabbit anti-mouse immunoglobulin (DAKO) diluted2,000 times as the secondary antibody.

The anti-C-hsi13412/C-kaia397 monoclonal antibody KM2961 and KM2962 werefound to specifically recognize proteins encoded by C-hsi13412 orC-kaia397 expressed in COS-1 cells of Example 8 (FIG. 11).

Example 15 Binding of IGF and Proteins Encoded by C-hsi13412 orC-kaia397

To investigate the binding affinity of the proteins encoded byC-hsi13412 and C-kaia397 to IGF, analysis by far-Western (MolecularCloning—A Laboratory Manual, Third Edition, Cold Spring HarborLaboratory; Protein Experiment Protocol 1, Shujunsha) was carried outusing the cell lysates of COS-1/C-hsi13412 cell line, COS-1/C-kaia397,and, as a control, COS-1/mock cell line obtained in Example 8.

A 5 μl aliquot of the cell lysates of COS-1/mock cell line,COS-1/C-hsi13412 cell line, and COS-1/C-kaia397 cell line was placedinto each lane, and according to Example 14, SDS-PAGE was carried out,transferred to a PVDF membrane, and then blocked. Subsequently, 2 μg/mlhuman IGF-I (Peprotech), 2 μg/ml human IGF-II (Peprotech), or 2 μg/ml ofhuman insulin (Sigma) diluted with the blocking solution was enclosedwith the membrane, and was allowed to react at room temperatureovernight. Next, anti-human IGF-I monoclonal antibody (UpstateBiotechnology), anti-human IGF-II monoclonal antibody (UpstateBiotechnology), or anti-human insulin polyclonal antibody (Santa Cruz)were added to each of the membranes to a concentration of 2 μg/ml, andwas left standing at room temperature for 1 hour. After the membrane waswashed well with Tween-TBS, detection was carried out usingperoxiase-labeled rabbit anti-mouse immunoglobulin (DAKO) diluted 2,000times as the secondary antibody.

With the anti-IGF-I antibody and anti-IGF-II antibody, bands of amolecular weight of approximately 33 kDa and approximately 22 kDaderived from proteins encoded by C-hsi13412 and C-kaia397, respectively,were detected. However, when anti-insulin antibody was used, these bandswere not detected. Therefore, the proteins encoded by C-hsi13412 orC-kaia397 were suggested to bind to IGF-I and IGF-II, but not to insulin(FIG. 12).

Example 16 Effects of Proteins Encoded by C-hsi13412 or C-kaia397 onCancer Cells

To investigate the action of C-hsi13412 and C-kaia397 on cancer cellgrowth, effects on IGF-dependent growth of human colon cancer cell lineHT-29 (ATCC HTB-38) were examined as described below using the culturesupernatants of COS-1/mock cell line, COS-1/C-hsi13412 cell line, andCOS-1/C-kaia397 cell line obtained in Example 8.

50 μl/well of HT-29 cells that were prepared at a concentration of 1×10⁵cells/ml in D-MEM/F-12 medium (Gibco) supplemented with 200 μg/ml ofbovine serum albumin (Gibco), 10 μg/ml of human transferrin (Gibco), 50units/ml penicillin (Gibco), and 50 μg/ml of streptomycin (Gibco), wereplated onto a 96-well plate. Cultivation was carried out at 37° C. in aCO₂ incubator for 3 hours. Next, human IGF-I (Peprotech) or human IGF-II(Peprotech) each at a final concentration of 0.1 ng/ml was addedthereto. Subsequently, 5 μl/well of the culture supernatant ofCOS-1/mock cell line, COS-1/C-hsi13412 cell line, or COS-1/C-kaia397cell line was added thereto. After cultivating at 37° C. in a CO₂incubator for an additional 3 days, viable cells were counted using CellProliferation Reagent WST-1 (Roche Diagnostics).

The culture supernatants of COS-1/C-hsi13412 cell line andCOS-1/C-kaia397 cell line were shown to inhibit IGF-dependent growth ofhuman colon cancer cell line HT-29 cells (FIG. 13).

INDUSTRIAL APPLICABILITY

According to the present invention, novel insulin-like growth factorbinding proteins, DNAs encoding the proteins, and antibodies recognizingthe proteins, as well as determination methods, diagnostic agents,preventive agents, and therapeutic agents for diseases associated withthe proteins can be provided.

1. An isolated antibody that binds to a polypeptide consisting of the171st to the 197th amino acid residues in the amino acid sequencerepresented by SEQ ID NO:
 2. 2. A method for producing the antibody ofclaim 1, wherein a polypeptide comprising an amino acid sequence thatincludes at least 5 or more continuous amino acids of the 171st to the197th amino acid residues in the amino acid sequence represented by SEQID NO: 2 is used as an antigen.
 3. An antibody obtained by the method ofclaim
 2. 4. A method for immunologically detecting or quantifying aninsulin-like growth factor binding protein, the method comprisingcontacting a sample suspected of comprising the protein with theantibody of claim 1, and immunologically detecting or quantifying theprotein.
 5. A medicament containing the antibody according to claim 1.6. The antibody according to claim 1, wherein the antibody does notrecognize an insulin-like growth factor binding protein which comprisesthe amino acid sequence represented by SEQ ID NO:
 1. 7. A monoclonalantibody produced by a hybridoma with the accession number FERM BP-7604.8. A method for immunologically detecting or quantifying an insulin-likegrowth factor binding protein, the method comprising contacting a samplesuspected of comprising the protein with a monoclonal antibody producedby a hybridoma with the accession number FERM BP-7604, andimmunologically detecting or quantifying the protein.
 9. A medicamentcontaining a monoclonal antibody produced by a hybridoma with theaccession number FERM BP-7604.